Molecular Characterization of a Novel Cathepsin L in Macrobrachium nipponense and Its Function in Ovary Maturation

Cathepsin L genes, which belonged to cysteine proteases, were a series of multifunctional protease and played important roles in a lot of pathological and physiological processes. In this study, we analyzed the characteristics a cathepsin L (named Mn-CL2) in the female oriental river prawn, Macrobrachium nipponense which was involved in ovary maturation. The Mn-CL2 was1,582 bp in length, including a 978 bp open reading frame that encoded 326 amino acids. The Mn-CL2 was classified into the cathepsin L group by phylogenetic analysis. Real-time PCR (qPCR) analysis indicated that Mn-CL2 was highly expressed in the hepatopancreas and ovaries of female prawns. During the different ovarian stages, Mn-CL2 expression in the hepatopancreas and ovaries peaked before ovarian maturation. In situ hybridization studies revealed that Mn-CL2 was localized in the oocyte of the ovary. Injection of Mn-CL2 dsRNA significantly reduced the expression of vitellogenin. Changes in the gonad somatic index also confirmed the inhibitory effects of Mn-CL2 dsRNA on ovary maturation. These results suggest that Mn-CL2 has a key role in promoting ovary maturation.

Gonad maturity level and somatic index of sea cucumber Acaudina sp. (caudinidae, molpadida, holothuroidea) In Delta Wulan Waters, Demak Regency, Indonesia

Sea cucumber Acaudina sp. belongs to the family Caudinidae, Order Molpadida, Class Holothuroidea. These animals have an important role in the benthic ecosystem. The purpose of this research was to determine the gonad maturity level and Gonad Somatic Index of Acaudina sp. in Delta Wulan Waters, Demak Regency. In total, 64 individuals of Acaudina sp. were taken in April 2021 and 148 individuals were taken in May 2021. In April observation, the highest gonad maturity level for females was at level 3, while the highest gonad maturity for male was at levels 1 and 2. Furthermore, in Mei observations, the highest gonad maturity level for females was at level 2 and for males, the highest gonad maturity level was at level 3. According to April observation, values GSI (Gonad Somatic Index) has a range 0.22 - 6.09 % and for May observation, value GSI has a range 0.04 – 2.24%. Hence, there is a positive correlation between gonad weight and GSI values in sea cucumber Acaudina sp.

Age, Growth, Condition Factor and Reproduction of Planiliza abu (Mugiliformes: Mugilidae) in the Al-Diwaniya River, Middle of Iraq

The age, growth, relative condition factor, and reproduction of Planiliza abu (Heckel, 1843) from the Al-Diwaniya River, Iraq was described in samples obtained from October 2016 to September 2017, using different fishing gears. Total length and weight were measured, scales were used for age determination, and gonads were excised from the body cavity sexed and weighed. The lengths of the species ranged from 7.0 to 20.0 cm, and the most dominant length group was 13.0 cm. The length-weight relationship was W=0.0199*L2.821 revealing a negative allometric growth (b= 2.821). The mean relative condition factor (Kn) was calculated as 1.13 for females and 0.93 for males. Four ages were recognized for the species with mean total lengths of 10.2, 14.5, 39.0, 17.2 and 19.3 cm, respectively. The von Bertalanffy growth parameters based on back-calculated lengths were L∞= 23.4 cm, K=0.38 and to= -0.27. The growth performance index (Ø) of the species is computed as 2.32. The overall sex ratio (male: female) was 1:1.70. The gonad-somatic index (GSI) values of both sexes were highest in April, 8.5 for males and 11.6 for females and the lowest values in August, 0.22 for males and 0.75 for females, indicated that the spawning period was from April to May. Some biological properties such as lengths of individuals, negative allometric growth, ages from 1 to 4 years, values of ultimate growth (L∞) and growth performance index (Ø) and overall sex ratio were among those described for the species in other waters. These results can contribute to providing information for species management in the study river.

Effect of Giving Different Doses of Vitamin E in Feed to the Level of Gonad Maturity of Tilapia (Oreochromis niloticus)

One of the nutrients that can be given to brood feed fish to improve reproductive performance and egg quality is vitamin E. Vitamin E has a very important role in increasing fish reproduction because vitamin E functions as an antioxidant that can maintain the presence of fatty acids and prevent fat oxidation in the fish cell membrane and can accelerate the secretion of reproductive hormones. This study aims to determine the effect of vitamin E in feed at different doses on the level of gonad maturity of tilapia. The research was conducted at the Fisheries Laboratory of the Faculty of Marine and Fisheries, Udayana University from July to August 2020. The samples of fish tested were tilapia (Oreochromis niloticus) obtained from the Teratai fishing pond, Sanur Bali. Vitamin E used was Ovagrow Vit E with a vitamin E content of 50%. The CRD method (completely randomized design) was used with four treatments and three replications. The doses of vitamin E that were mixed into the feed were 0, 100, 300, and 500 mg/kg of feed. The results showed that the administration of vitamin E mixed in the feed at different doses had a significant effect on the achievement of gonad maturity level, gonad somatic index (GSI), and Fecundity (P <0.05). Provision of vitamin E at a dose of 300 mg/kg of feed was the best dose for ripening tilapia gonads. The gonad maturity level developed up to maturity stage IV, the GSI value was (3.56 ± 0.46) and the fecundity value was (1379 ± 289).

Reproductive activity and morphometric assessment of three commercial species of sea cucumber (Echinodermata) from Karimunjawa National Park, Indonesia

Mustagfirin, Wijayanti DP, Subagiyo. 2021. Reproductive activity and morphometric assessment of three commercial species of sea cucumber (Echinodermata) from Karimunjawa National Park, Indonesia. Biodiversitas 22: 3333-3341. Sea cucumbers are benthic animals that have economic value and play an important role in the environment. Exploitation on large scale to fulfill high market demand, caused overexploitation. The number of sea cucumbers in Karimunjawa decreased as a result of limited management. There is no study conducted on the commercial sea cucumbers (Holothuria edulis, Pearsonothuria graeffei, and Bohadschia vitensis) in Nyamuk Island, Karimunjawa. The objectives of the research were to determine the morphometric assessment and the reproductive cycle of the three species. The morphometric assessment of weight showed that the weight of H. edulis ranges 247.3-2539g, P. graeffei 415.3-428.1g, and B. vitiensis 672.4-678.1g, respectively. The first maturity size of gutted body weight (GWB50) for H. edulis was 127.84g, P. graeffei 357.61g, and B. vitiensis 279.34g. The gonad somatic index (GSI) range 13.60-15.60% for H. edulis, 14.75-12.25% for P. graeffei and 10.83-14.57% for B. vitiensis. The result of gonad somatic index with a value above 10% means that it has entered the spawning phase. The above data indicated that the three commercial species spawned monthly, during new moon. It is likely that the spawning peak occurred when the temperature was increased. However, the pattern of reproductive activity in this study was limited at Nyamuk Island. Future studies are needed to observe whether there is a correlation between the increasing water temperature and the reproductive activity of the three sea cucumbers.

Biologi reproduksi udang mantis Cloridopsis scorpio di ekosistem mangrove Belawan, Sumatera Utara

Anthropogenic activities that tend to be exploitative in the mangrove ecosystem of Belawan are thought to have an impact on the biota associated with the ecosystem. One of the impacts is thought to be implied by mantis shrimp Cloridopsis scorpio (Latreile, 1828). The reproductive biology of the shrimp is the main object that needs to be studied in relation to the impacts. This study aims to determine the distribution of gonad maturity stage, gonad somatic index, and the length of the first mature of C. scorpio in the Mangrove Ecosystem of Belawan, North Sumatera. The research was conducted on March to May 2019 with a biweekly sampling period. The purposive random sampling was applied to choose three sampling stations in the research location. Mantis shrimp samples were caught using shrimp trawl. The result showed that the gonad maturity stage of males and females were dominated by Stage I. The gonad somatic index (GSI) range of males were 7.00-10.93 and female were 7.40-11.15. The GSI value of C. scorpio is closely related to its gonad maturity development. The length of the first mature (L50) of males were 205.50±10.65 mmBL and females were 186.0±10.48 mmBL.Keywords: Cloridopsis scorpio, Gonad maturity stage, Gonad somatic indeks, Length of the first mature ABSTRAKAktivitas antropogenik yang cenderung eksploitatif di kawasan ekosistem mangrove Belawan diduga telah berdampak terhadap biota-biota yang berasosiasi dengan ekosistem tersebut. Salah satu dampaknya dialami oleh udang mantis Cloridopsis scorpio (Latreile, 1828). Aspek reproduksi C. scorpio menjadi salah satu objek yang perlu dikaji berkaitan dengan dampak tersebut. Penelitian ini bertujuan untuk menganalisis sebaran tingkat kematangan gonad (TKG), indeks kematangan gonad (IKG), dan ukuran pertama kali matang gonad (L50) dari C. scorpio di ekosistem mangrove Belawan, Sumatera Utara. Penelitian dilakukan pada bulan Maret sampai Mei 2019 dengan periode pengambilan sampel dua mingguan. Metode purposive random sampling digunakan untuk menentukan tiga stasiun pengambilan sampel di lokasi penelitian. Sampel C. scorpio ditangkap menggunakan pukat udang. Hasil penelitian menunjukkan distribusi TKG C. scorpio jantan dan betina didominasi oleh TKG I. Nilai IKG C. scorpio jantan berkisar antara 7,00-10,93 dan C. scorpio betina berkisar antara 7,40-11,15. Nilai IKG C. scorpio berkaitan dengan tahap perkembangan gonadnya. C. scorpio jantan mengalami pertama kali matang gonad (L50) pada ukuran 205,50±10,65 mmBL dan C. scorpio betina pada ukuran 186,00±10,48 mmBL.Kata kunci: Cloridopsis scorpio, TKG, IKG, Ukuran pertama kali matang gonad

Analisis Konsentrasi Limbah Rumah Pemotongan Unggas (RPU) terhadap Pertumbuhan dan Indeks Fisiologi Ikan Nila (Oreochromis niloticus) Skala Laboratorium Concentration of Poultry Slaughterhouse Waste on Growth and Physiology Index of Tilapia (Oreochromis niloticus) in Laboratory Scale

ABSTRAKPertumbuhan ikan tergantung dari kondisi perairan tempat hidupnya. Limbah dari aktivitas pemotongan unggas yang dibuang berpotensi mencemari perairan. Penelitian ini bertujuan untuk mengetahui pengaruh limbah Rumah Pemotongan Unggas (RPU) terhadap pertumbuhan dan indeks fisiologi Ikan Nila (Oreochromis niloticus). Penelitian dilaksanakan pada bulan November 2018 – Januari 2019. Penelitian menggunakan metode eksperimen dengan pola Rancangan Acak Lengkap (RAL). Air limbah RPU diambil di rumah pemotongan ayam Tembalang. Teknik pengambilan data dilakukan dengan cara observasi langsung. Rancangan percobaan penelitian ini menggunakan Ikan Nila dengan bobot 2 – 3 gr, diuji selama 28 hari menggunakan konsentrasi limbah berturut-turut 0% v/v, 10% v/v, 20% v/v, 30% v/v, dan 40% v/v dari nilai LC50 – 96 Jam sebesar 129,865 ml/. Parameter yang dianalisis meliputi pertumbuhan biomassa mutlak, indeks fisiologi, dan kualitas air yang terdiri dari DO, pH, dan temperatur. Hasil pengujian limbah RPU terhadap pertumbuhan Ikan Nila menunjukkan rata-rata pertumbuhan berat ikan tertinggi ada pada konsentrasi D (12,986 ml/l), yaitu 4,71 gram, dan pertumbuhan terendah pada konsentrasi A (51,946 ml/l), yaitu 1,51 gram. Hasil pengujian limbah RPU terhadap CF (Condition Factor) yaitu 0 ml/l adalah 3,10; 51,946 ml/l adalah 3,88; 38,959 ml/l adalah 4,02; 25,973 ml/l adalah 2,84; dan 12,986 ml/l adalah 3,09. Hasil LSI berturut-turut yaitu 0 ml/l rata-adalah 1,20; 51,946 ml/l adalah 3,65; 38,959 ml/l adalah 3,06; 25,973 ml/l adalah 2,07; dan 12,986 ml/l adalah 1,41. Hasil GSI (Gonad Somatic Index) berturut-turut yaitu 0 ml/l adalah 0,50 ; 51,946 ml/l adalah 0,20 ; 38,959 ml/l adalah 0,35; 25,973 ml/l adalah 0,35; dan 12,986 ml/l adalah 0,42. ABSTRACTFish's growth depends on the water condition of their habitat. Waste in the form of slaughtered poultry slaughtering activities has the potential to pollute the waters. This study aims to determine the effect of Poultry Slaughterhouse Waste on the growth and physiological index of Tilapia (Oreochromis niloticus). The study was conducted in November 2018 - January 2019. The method used was an experimental method with a completely randomized design pattern. Slaughterhouse waste water is taken at Tembalang chicken slaughterhouses. The data collection method uses direct observation. The experimental design of this study was using Tilapia with a weight of 2-3 grams, tested for 28 days using concentrations of waste respectively 0% v/v, 10% v/v, 20% v/v, 30% v/v, and 40% v/v of the LC50 - 96 Hours value of 129,865 ml / L. Parameters analyzed included absolute biomass growth, physiological index, and water quality consisting of DO, pH, and temperature. The results of slaughterhouse waste testing on the growth of Tilapia showed that the highest average weight growth of fish was at concentration D (12,986 ml / l), which was 4,71 gram, and the lowest growth was at concentration A (51,946 ml / l), which was 1,51 gram. The results of slaughterhouse's waste testing on CF (Condition Factor) that is 0 ml / l are 3,10; 51,946 ml / l is 3,88; 38,959 ml / l is 4,02; 25,973 ml / l is 2,84; and 12,986 ml / l is 3,09. The consecutive LSI results, ie 0 ml / l, were 1.20; 51,946 ml / l is 3.65; 38,959 ml / l is 3.06; 25,973 ml / l is 2.07; and 12,986 ml / l is 1.41. The result of GSI (Gonad Somatic Index) in a row that is 0 ml / l is 0.50; 51,946 ml / l is 0.20; 38,959 ml / l is 0.35; 25,973 ml / l is 0.35; and 12,986 ml / l is 0.42.

THE EFFECT OF KATUK (Sauropus androgynus L. Merr) ETHANOL EXTRACT ON OVARIAN MATURITY OF MUD CRAB (Scylla sp)

Katuk leaves are plants that contain sterols. Sterols are steroid precursors used for the development of mud crab ovaries. This study aims to determine the development of mangrove crab ovaries by giving katuk leaf extract. Stages of research include katuk leaf extraction, experiments by injecting the mother crab consisting of 3 treatments consisting of treatment A (control), treatment B (0.25 mg / g body weight), treatment C (0.5 mg / g body weight) ) maintained for 20 days by feeding trash fish and measuring parameters including morphological ovarian development, gonad somatic index, and hepatosomatic index. The results of the study on C treatment on the 20th day of observation by ovarian morphology have developed in the TKO IV phase, the final B TKO III treatment and the A-phase TKO III treatment. GSI on the 20th day in treatment C showed the highest GSI value of 16.1% and the lowest in treatment A was 12.2%. It can be concluded that the administration of katuk extract can accelerate the ovarian maturity of mud crabs.

TO THE METHODOLOGY FOR SEPARATION OF MIGRATING POPULATION COMPLEXES OF THE OKHOTSK SEA PINK SALMON IN THE PACIFIC WATERS AT KURIL ISLANDS USING THE GONAD-SOMATIC INDEX

New method is proposed for operational differentiation of pink salmon runs with different time of spawning and different state of gonads measured quantitatively with a gonad-somatic index. Results of this new method and traditional methods of differentiating are compared. The conception of spatial niches in the Pacific waters adjacent to Kuril Islands for certain temporal groupings of the Okhotsk Sea pink salmon is critically revised on the base of these new results. The runs cannot be separated using any fixed value of the gonad-somatic index. The zones of different groupings could be spatially overlapped. The method is tested on the data of trawl survey conducted aboard RV Professor Kaganovsky in May-July 2018, during anadromous migration of pink salmon, and shows a high compliance with the regional groupings ratio determined on the data of pink salmon catch in the Okhotsk Sea and their escapement to rivers. Tendency of the late-maturing forms of pink salmon to migrate through the southern Kuril waters is confirmed, whereas the portion of early-maturing pink has increased toward the Ocean.

SOME BIOLOGICAL ASPECTS OF SKIPJACK TUNA (Katsuwonus pelamis LINNAEUS, 1758) DI PERAIRAN TOLI-TOLI WATERS, CENTRAL SULAWESI

Skipjack tuna (Katsuwonus pelamis) was one of the export commodities, where the demand tends to increase. The high level of skipjack tuna exploitation leads to overfishing, so sustainable fisheries management was required. The objective of the research was to study the several aspects of biology including of length of weight relationship, sex ratio maturity stage, gonad somatic index (GSI), Length at first capture and length at first maturity. The research was conducted in February - December 2015 in Toli-toli, Central Sulawesi. The results showed that the skipjack tuna growth pattern was allometrically positive, where growth weight was faster than the length increase. The length of the first time capture skipjack tuna of pole and line was bigger than length at first maturity, it means the mostly skipjack tuna caught have already spawned. The spawning season was occur throughout the year with spawning peaks in April and September, fecundity ranging from 450,570 - 1,707,390eggs.