scholarly journals A sensitive and specific point-of-care detection assay for Zaire Ebola virus

2017 ◽  
Vol 6 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Xiao-Ai Zhang ◽  
Sabrina Li ◽  
Jesus Ching ◽  
Hui-Ying Feng ◽  
Kun Yang ◽  
...  
Keyword(s):  
Ebola Virus ◽  
Point Of Care ◽  
Specific Point ◽  
Detection Assay ◽  
Point Of Care Detection

Homogeneous and high-density gold unit implanted optical labels for robust and sensitive point-of-care drug detection

Nanoscale ◽  
2019 ◽  
Vol 11 (34) ◽  
pp. 16026-16035 ◽  
Author(s):  
Liang Huang ◽  
Jiening Jin ◽  
Jing Wang ◽  
Chenxing Jiang ◽  
Meng Xu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Point Of Care ◽  
High Density ◽  
Specific Point ◽  
Drug Detection ◽  
Optical Labels ◽  
Point Of Care Detection

Sensitive and specific point-of-care detection of methamphetamine in urine was achieved using novel structured gold nano-labels for signal amplification.

scholarly journals A Multiplexed Microfluidic PCR Assay for Sensitive and Specific Point-of-Care Detection of Chlamydia trachomatis

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e51685 ◽  
Author(s):  
Deborah Dean ◽  
Rosemary S. Turingan ◽  
Hans-Ulrich Thomann ◽  
Anna Zolotova ◽  
James Rothschild ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Point Of Care ◽  
Specific Point ◽  
Pcr Assay ◽  
Point Of Care Detection

scholarly journals Molecular Diagnostic Field Test for Point-of-Care Detection of Ebola Virus Directly From Blood

2016 ◽  
Vol 214 (suppl 3) ◽  
pp. S234-S242 ◽  
Author(s):  
Jason W. Benzine ◽  
Kerry M. Brown ◽  
Krystle N. Agans ◽  
Ronald Godiska ◽  
Chad E. Mire ◽  
...  
Keyword(s):  
Field Test ◽  
Ebola Virus ◽  
Point Of Care ◽  
Molecular Diagnostic ◽  
Point Of Care Detection

Genosensor for rapid, sensitive, specific point-of-care detection of H1N1 influenza (swine flu)

2020 ◽  
Vol 98 ◽  
pp. 262-268
Author(s):  
Ravina ◽  
Anita Dalal ◽  
Paramjeet Singh Gill ◽  
Jagriti Narang ◽  
Minakshi Prasad ◽  
...  
Keyword(s):  
Point Of Care ◽  
H1n1 Influenza ◽  
Swine Flu ◽  
Specific Point ◽  
Point Of Care Detection

scholarly journals Rapid and sensitive point-of-care detection of Leptospira by RPA-CRISPR/Cas12a targeting lipL32

2022 ◽  
Vol 16 (1) ◽  
pp. e0010112
Author(s):  
Sirawit Jirawannaporn ◽  
Umaporn Limothai ◽  
Sasipha Tachaboon ◽  
Janejira Dinhuzen ◽  
Patcharakorn Kiatamornrak ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Clinical Performance ◽  
Point Of Care ◽  
Limited Resource ◽  
Cross Reactivity ◽  
Clinical Samples ◽  
Detection Assay ◽  
Short Palindromic Repeat ◽  
Flow Detection ◽  
Point Of Care Detection

Background One of the key barriers preventing rapid diagnosis of leptospirosis is the lack of available sensitive point-of-care testing. This study aimed to develop and validate a clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 12a (CRISPR/Cas12a) platform combined with isothermal amplification to detect leptospires from extracted patient DNA samples. Methodology/Principal findings A Recombinase Polymerase Amplification (RPA)-CRISPR/Cas12a-fluorescence assay was designed to detect the lipL32 gene of pathogenic Leptospira spp. The assays demonstrated a limit of detection (LOD) of 100 cells/mL, with no cross-reactivity against several other acute febrile illnesses. The clinical performance of the assay was validated with DNA extracted from 110 clinical specimens and then compared to results from qPCR detection of Leptospira spp. The RPA-CRISPR/Cas12a assay showed 85.2% sensitivity, 100% specificity, and 92.7% accuracy. The sensitivity increased on days 4–6 after the fever onset and decreased after day 7. The specificity was consistent for several days after the onset of fever. The overall performance of the RPA-CRISPR/Cas12a platform was better than the commercial rapid diagnostic test (RDT). We also developed a lateral flow detection assay (LFDA) combined with RPA-CRISPR/Cas12a to make the test more accessible and easier to interpret. The combined LFDA showed a similar LOD of 100 cells/mL and could correctly distinguish between known positive and negative clinical samples in a pilot study. Conclusions/Significance The RPA-CRISPR/Cas12 targeting the lipL32 gene demonstrated acceptable sensitivity and excellent specificity for detection of leptospires. This assay might be an appropriate test for acute leptospirosis screening in limited-resource settings.

Dual-Signal Readout Nanospheres for Rapid Point-of-Care Detection of Ebola Virus Glycoprotein

2017 ◽  
Vol 89 (24) ◽  
pp. 13105-13111 ◽  
Author(s):  
Jiao Hu ◽  
Yong-Zhong Jiang ◽  
Ling-Ling Wu ◽  
Zhen Wu ◽  
Yuhai Bi ◽  
...  
Keyword(s):  
Ebola Virus ◽  
Point Of Care ◽  
Virus Glycoprotein ◽  
Point Of Care Detection ◽  
Signal Readout

scholarly journals Emerging Options for the Diagnosis of Bacterial Infections and the Characterization of Antimicrobial Resistance

2021 ◽  
Vol 22 (1) ◽  
pp. 456
Author(s):  
Simone Rentschler ◽  
Lars Kaiser ◽  
Hans-Peter Deigner
Keyword(s):  
Antimicrobial Resistance ◽  
Bacterial Infections ◽  
Point Of Care ◽  
Rapid Identification ◽  
Adequate Treatment ◽  
Resistance Patterns ◽  
Detection And Diagnosis ◽  
Patient Prognosis ◽  
Point Of Care Detection

Precise and rapid identification and characterization of pathogens and antimicrobial resistance patterns are critical for the adequate treatment of infections, which represent an increasing problem in intensive care medicine. The current situation remains far from satisfactory in terms of turnaround times and overall efficacy. Application of an ineffective antimicrobial agent or the unnecessary use of broad-spectrum antibiotics worsens the patient prognosis and further accelerates the generation of resistant mutants. Here, we provide an overview that includes an evaluation and comparison of existing tools used to diagnose bacterial infections, together with a consideration of the underlying molecular principles and technologies. Special emphasis is placed on emerging developments that may lead to significant improvements in point of care detection and diagnosis of multi-resistant pathogens, and new directions that may be used to guide antibiotic therapy.

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