Bacterial metabolism of methylated amines and identification of novel methylotrophs in Movile Cave

Facultative methylotrophs belonging to the genera Gemmobacter and Mesorhizobium were isolated from microbial mat and cave water samples obtained from the Movile Cave ecosystem. Both bacteria can utilize methylated amines as their sole carbon and nitrogen source. Here, we report the draft genome sequences of Gemmobacter sp. strain LW1 and Mesorhizobium sp. strain IM1.

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Comparative genomics analyses indicate differential methylated amine utilisation trait within members of the genusGemmobacter

10.1101/356014 ◽

2018 ◽

Author(s):

E. Kröber ◽

J Peixoto ◽

MR Cunningham ◽

L. Spurgin ◽

D Wischer ◽

...

Keyword(s):

Comparative Genomics ◽

Carbon Source ◽

Microbial Degradation ◽

The Public ◽

Genetic Potential ◽

Cave Waters ◽

Public Repositories ◽

Earth’S Climate ◽

Movile Cave ◽

Methylated Amines

AbstractMethylated amines are ubiquitous in the environment and play a role in regulating the earth’s climate via a set of complex biological and chemical reactions. Microbial degradation of these compounds is thought to be a major sink. Recently we isolated a facultative methylotroph,Gemmobactersp. LW-1, an isolate from the unique environment Movile Cave, Romania, which is capable of methylated amine utilisation as a carbon source. Here, using a comparative genomics approach, we investigate how widespread methylated amine utilisation trait is within the member of the bacterial genusGemmobacter. Five genomes of differentGemmobacterspecies isolated from diverse environments, such as activated sludge, fresh water, sulphuric cave waters (Movile Cave) and the marine environment were available from the public repositories and used for the analysis. Our results indicate that some members of the genusGemmobacter, namelyG. aquatilis, G. caeniandG. sp. LW-1 have the genetic potential of methylated amine utilisation while others (G. megateriumandG. nectariphilus) have not.

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Pilot Study of Probiotic Supplementation on Uremic Toxicity and Inflammatory Cytokines in Chronic Kidney Patients

Current Nutrition & Food Science ◽

10.2174/1573401315666190215111402 ◽

2020 ◽

Vol 16 (4) ◽

pp. 470-480

Author(s):

Cristina T. Roth-Stefanski ◽

Carla Dolenga ◽

Lia S. Nakao ◽

Roberto Pecoits-Filho ◽

Thyago P. de Moraes ◽

...

Keyword(s):

Pilot Study ◽

Inflammatory Cytokines ◽

Lactobacillus Acidophilus ◽

Serum Levels ◽

Uremic Toxins ◽

Indoxyl Sulfate ◽

Bacterial Metabolism ◽

Probiotic Supplementation ◽

Primary Endpoints ◽

Inflammatory Profile

Background: Bacterial metabolism contributes to the generation of uremic toxins in patients with chronic kidney disease (CKD). It has been investigated the use of probiotics in the reduction of uremic toxins intestinal production. Objective: The aim of this pilot study was to evaluate the effect of probiotic supplementation on reducing the production of uremic toxins and the inflammatory profile of CKD patients. Methods: We performed a randomized, blind, placebo-controlled, crossover study on patients with CKD stages 3 and 4. The intervention was a probiotic formulation composed of Lactobacillus acidophilus strains given orally three times a day for 3 months. Changes in uremic toxins (p-Cresylsulfate and Indoxyl Sulfate) and serum inflammatory cytokines were the primary endpoints. Results: Of the 44 patients randomized, 25 completed the study (mean age 51 ± 9.34, 64% female, mean eGFR 36 ± 14.26 mL/min/1.73m², mean BMI 28.5 ± 5.75 kg/m²). At 3 months, there were no significant changes in any of the studied biomarkers including p-cresylsulfate (p = 0.57), Indoxyl sulfate (p = 0.08) and interleukin-6 (p = 0.55). Conclusion: Lactobacillus acidophilus strains given as probiotic were not able to reduce serum levels of uremic toxins and biomarkers of inflammation in CKD patients in stage 3 and 4.

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