Effect of different agents with potential antibiofilm activity on antimicrobial susceptibility of biofilms formed by Staphylococcus spp. isolated from implant-related infections

2016 ◽  
Vol 69 (9) ◽  
pp. 686-688 ◽  
Author(s):  
Diana Molina-Manso ◽  
Gema Del-Prado ◽  
Enrique Gómez-Barrena ◽  
Jose Cordero-Ampuero ◽  
Ricardo Fernandez-Roblas ◽  
...  
2021 ◽  
Vol 10 (4) ◽  
pp. 377-383
Author(s):  
Murad A. Hiblu ◽  
Omar M. Ellraiss ◽  
Elfurgani S. Karim ◽  
Rabia A. Elmishri ◽  
Enass M. Duro ◽  
...  

Background: Feline otitis externa is a dermatological disorder with a multifactorial complex etiology.Aim: This study aimed to investigate the prevalence of different etiological agents, particularly the parasitic and bacterial, responsible for the cases of feline otitis externa in Tripoli, Libya, and to assess the antimicrobial susceptibility of the bacterial isolates from those cases.Methods: Cerumen and otic discharges of the suspected cats were collected for parasite detection and bacterial culture. Kirby–Bauer’s disk diffusion method was used for antimicrobial susceptibility testing.Results: The results showed that otodectic mites and bacterial causes were equally the most prevalent in those cases, with a prevalence of 47.1% each. Otodectes cynotis infestation was more frequently bilateral and severe. Staphylococcus spp. were the most prevalent among bacterial causes (75%), followed by Proteus spp. (16.6%) and Pseudomonas spp. (8.4%). Norfloxacin and gentamicin were the most effective antimicrobials against bacterial isolates, as they were effective against 83.3% and 70.8% of isolates, respectively.Conclusion: Otodectes cynotis infestation and staphylococcal infections constituted the most common etiology of feline otitis externa in Tripoli, Libya, and norfloxacin represented a cogent antibacterial for the treatment of otitis externa.


2013 ◽  
Vol 52 (2) ◽  
pp. 392-397 ◽  
Author(s):  
A. M. Bobenchik ◽  
J. A. Hindler ◽  
C. L. Giltner ◽  
S. Saeki ◽  
R. M. Humphries ◽  
...  

2017 ◽  
Vol 20 (2) ◽  
pp. 122 ◽  
Author(s):  
Fernanda Freire ◽  
Cristiane Aparecida Pereira ◽  
Luciane Dias Oliveira ◽  
Juliana Campos Junqueira ◽  
Antonio Olavo Cardoso Jorge

<p><strong>Objective</strong>: The aim of this study was to identify the slime production and evaluate the effects of <em>Rosmarinus officinalis</em> (rosemary) and <em>Syzygium cumini</em> (jambolan) glycolic extracts, and 0.12% chlorhexidine (CHX) in biofilms formed by strains of coagulase-positive <em>Staphylococcus</em> - CPS and coagulase negative <em>Staphylococcus</em> - CNS isolated from the oral cavity. <strong>Material</strong> <strong>and</strong> <strong>Methods</strong>: Slime production was evaluated by two methods: the color of colony presented in Congo red agar, and through the amount of slime adhered to polystyrene. Biofilms were grown in acrylic resin discs immersed in broth, inoculated with microbial suspension (10<sup>6</sup> cells/ml) and incubated at 37°C/48 h. After formation, the biofilms were exposed for 5 minutes to glycol extracts, CHX or saline solution. The viability of biofilms was determined by counting the colony-forming units per milliliter (CFU/ml) in agar, and analyzed statistically by Tukey test (p &lt;0.05). <strong>Results</strong>: The strains <em>S. aureus</em>, <em>S. schleiferi</em> and <em>S. epidermidis</em> obtained the highest values of slime adhered to polystyrene. <em>R. officinalis</em> promoted reductions ranging from 12.1% to 78.7% in biofilms formed by isolates of CPS, and 9.2% to 73.7% in the biofilms of CNS. <em>S. cumini</em> reduced 12% to 55.7% in biofilms of CPS, and 7.9% to 71.5% in biofilms of CNS. With exception of <em>S. saprophyticus</em>, glycol extracts produced significant reductions in biofilms. For five isolates studied, <em>R. officinalis</em> produced greater reductions than CHX. <strong>Conclusion</strong>:<em> R. officinalis</em> and <em>S. cumini</em> showed effective antibiofilm activity against isolates that showed slime production.</p><p><strong> </strong></p><p><strong>Keywords</strong></p><p>Biofilm; <em>Rosmarinus officinalis; </em>Slime; <em>Staphylococcus;</em> <em>Syzygium cumini</em>.</p>


2021 ◽  
Vol 15 (2) ◽  
pp. 110-118
Author(s):  
Jorge Bárcena Barriuso ◽  
Deivid Roni Ribeiro ◽  
Javier Felipe Burchard ◽  
Kung Darh Chi ◽  
Amanda Anater ◽  
...  

This study aimed to identify which are the most frequent bacteria evolved in cases of chronic otitis in dogs in the metropolitan region of Curitiba, as well to determine their in vitro antimicrobial susceptibility. Data of positive bacterial culture from dogs affected by chronic or recurrent otitis were compiled from the records of the veterinary hospital of Pontifícia Universidade Católica do Paraná, Curitiba, southern Brazil. In a period of 16 months, a total of 83 bacterial cultures were performed, resulting in 192 isolates. All isolates were submitted to antimicrobial susceptibility tests, based on the Kirby-Bauer technique using 17 drugs from 8 antibiotic classes (?-lactams, aminoglycosides, lincosamides, macrolides, polypeptides, quinolones, tetracyclines, and amphenicols). The five most frequent bacterial isolates were Staphylococcus spp. (58.32%), Proteus spp. (14.58%), Escherichia coli (9.90%) and Pseudomonas spp. (8.33%). The four most effective antibiotics were amikacin (13.29%), neomycin (24.47%), gentamicin (25.52%) and tobramycin (26.70%); however, these aminoglycosides may cause ototoxicity, and their use should be restricted when the tympanic membrane is intact. Quinolones also showed antimicrobial effectiveness, with 29.17% of the isolates showing resistance to ciprofloxacin and 29.69% to enrofloxacin. According to the results, it can be concluded that aminoglycosides and quinolones were effective against microorganisms of canine chronic otitis.


2016 ◽  
Vol 46 (12) ◽  
pp. 2148-2151 ◽  
Author(s):  
Isabela de Godoy ◽  
Danny Franciele da Silva Dias Moraes ◽  
Letícia Camara Pitchenin ◽  
Janaina Marcela Assunção Rosa ◽  
Francielle Cristina Kagueyama ◽  
...  

ABSTRACT: The aim of this study was to determine the prevalence and diversity of veterinary clinical isolates of Staphylococcus and analyze their antimicrobial susceptibility. One hundred Staphylococcus spp. clinical isolates from domestic and wild animals were subjected to partial sequencing of the 16S rRNA gene to species determination. Antimicrobial susceptibility was obtained by a disk diffusion test against six antibiotics: amoxicillin (AMX), cephalexin (LEX), ciprofloxacin (CIP), erythromycin (ERY), gentamicin (GEN) and trimethoprim-sulfamethoxazole (SXT). The most common specie was S. pseudintermedius (61%, 61/100) and resistance to ERY (57%, 57/100), SXT (50%, 50/100) and AMX (46%, 46/100) was detected most frequently. In total, 40% (40/100) of Staphylococcus spp. exhibited a multidrug-resistant (MDR) phenotype. Results of this study emphasize that animals are reservoir of MDR Staphylococcus spp.


PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94627 ◽  
Author(s):  
Eloiza H. Campana ◽  
Cecilia G. Carvalhaes ◽  
Bruna Nonato ◽  
Antonia M. de. O. Machado ◽  
Ana C. Gales

Author(s):  
George Cosmin NADĂȘ ◽  
Cristiana Ștefania NOVAC ◽  
Cosmina Maria BOUARI ◽  
Ioana Adriana MATEI ◽  
Nicodim Iosif FIȚ

Bovine mastitis is a cause of major financial losses in the dairy industry worldwide. Bovine mastitis is defined as the inflammatory response of the udder tissue due to either physical trauma or infections caused by different microorganisms, especially bacteria. More than 135 pathogenic agents have been identified from bovine mastitis. The aim of the study was to identify the bacterial genera involved in mastitis etiology in cattle and to test the antimicrobial susceptibility to antibiotics. The samples were collected before morning milking and evaluated by microscopic and cultural examination, and further identified using biochemical methods. The evaluation of antimicrobial susceptibility was performed using Kirby-Bauer disk diffusion test. Overall, Staphylococcus spp., Bacillus spp., Streptococcus spp., Micrococcus spp., Escherichia spp. and Trueperella spp. were identified. The most frequent bacterial associations were observed for Staphylococcus-Bacillus, Bacillus-Streptococcus and Staphylococcus-Micrococcus. Regarding the antimicrobial sensitivity profile, the majority of the bacterial strains we have isolated were classified as sensitive, with the exception of Escherichia coli, with one strain being resistant to all tested antibiotics. Among the antimicrobials, the Amoxicillin and clavulanic acid was the most efficient one and Trimethoprim/Sulfamethoxazole did not exhibit an inhibitory activity in 19 milk samples.


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