scholarly journals Modular construction of mammalian gene circuits using TALE transcriptional repressors

2015 ◽  
Vol 11 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Yinqing Li ◽  
Yun Jiang ◽  
He Chen ◽  
Weixi Liao ◽  
Zhihua Li ◽  
...  
2013 ◽  
Vol 41 (16) ◽  
pp. e156-e156 ◽  
Author(s):  
Patrick Guye ◽  
Yinqing Li ◽  
Liliana Wroblewska ◽  
Xavier Duportet ◽  
Ron Weiss
Keyword(s):  

2019 ◽  
Vol 52 ◽  
pp. 16-22 ◽  
Author(s):  
Hideyuki Nakanishi ◽  
Hirohide Saito
Keyword(s):  

2013 ◽  
Vol 17 (6) ◽  
pp. 910-917 ◽  
Author(s):  
Haifeng Ye ◽  
Dominique Aubel ◽  
Martin Fussenegger

2020 ◽  
Author(s):  
S. Pérez García ◽  
M. García Navarrete ◽  
D. Ruiz Sanchis ◽  
C. Prieto Navarro ◽  
M. Avdovic ◽  
...  

SummaryHow do dynamic hormone inputs translate into speed, and precision of response is one of the most challenging questions of science. To approach this question, we constructed minimal synthetic gene circuits capable of responding to plant hormones auxin and salicylic acid (SA). These circuits integrate bacterial multi antibiotic resistance (Mar) repressors that directly detect phytohormones through a ligand-induced conformational switch. The combination of individual circuits in synthetic auxin-SA crosstalk was sufficient to coordinate responses across the cell population with tunable precision and speed in long-term microfluidics experiments. This antagonistic auxin-SA crosstalk retains temporal memory upon extended exposure to hormones and synchronizes the behavior of individual cells with the environmental clock. Our study shows how dynamic hormone inputs can be translated in robust and precise responses with a minimal assembly of bacterial transcriptional repressors, suggesting an alternative regulatory strategy to known plant hormone signaling systems.


2017 ◽  
Author(s):  
TL Vu-Han ◽  
S Buhs ◽  
H Gerull ◽  
M Horstmann ◽  
P Nollau

Author(s):  
Tochukwu Moses ◽  
David Heesom ◽  
David Oloke ◽  
Martin Crouch

The UK Construction Industry through its Government Construction Strategy has recently been mandated to implement Level 2 Building Information Modelling (BIM) on public sector projects. This move, along with other initiatives is key to driving a requirement for 25% cost reduction (establishing the most cost-effective means) on. Other key deliverables within the strategy include reduction in overall project time, early contractor involvement, improved sustainability and enhanced product quality. Collaboration and integrated project delivery is central to the level 2 implementation strategy yet the key protocols or standards relative to cost within BIM processes is not well defined. As offsite construction becomes more prolific within the UK construction sector, this construction approach coupled with BIM, particularly 5D automated quantification process, and early contractor involvement provides significant opportunities for the sector to meet government targets. Early contractor involvement is supported by both the industry and the successive Governments as a credible means to avoid and manage project risks, encourage innovation and value add, making cost and project time predictable, and improving outcomes. The contractor is seen as an expert in construction and could be counter intuitive to exclude such valuable expertise from the pre-construction phase especially with the BIM intent of äóÖbuild it twiceäó», once virtually and once physically. In particular when offsite construction is used, the contractoräó»s construction expertise should be leveraged for the virtual build in BIM-designed projects to ensure a fully streamlined process. Building in a layer of automated costing through 5D BIM will bring about a more robust method of quantification and can help to deliver the 25% reduction in overall cost of a project. Using a literature review and a case study, this paper will look into the benefits of Early Contractor Involvement (ECI) and the impact of 5D BIM on the offsite construction process.


Sign in / Sign up

Export Citation Format

Share Document