scholarly journals Translational repression by a miniature inverted-repeat transposable element in the 3′ untranslated region

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Jianqiang Shen ◽  
Juhong Liu ◽  
Kabin Xie ◽  
Feng Xing ◽  
Fang Xiong ◽  
...  
Keyword(s):  
Transposable Element ◽  
Inverted Repeat ◽  
Untranslated Region ◽  
Translational Repression

scholarly journals Separate Elements in the 3′ Untranslated Region of the Mouse Protamine 1 mRNA Regulate Translational Repression and Activation during Murine Spermatogenesis

1997 ◽  
Vol 191 (1) ◽  
pp. 42-52 ◽  
Author(s):  
Mark A. Fajardo ◽  
Harald S. Haugen ◽  
Christopher H. Clegg ◽  
Robert E. Braun
Keyword(s):  
Untranslated Region ◽  
Translational Repression ◽  
Protamine 1

Temporal regulation of the Xenopus FGF receptor in development: a translation inhibitory element in the 3′ untranslated region

Development ◽  
1995 ◽  
Vol 121 (6) ◽  
pp. 1775-1785 ◽  
Author(s):  
E.P. Robbie ◽  
M. Peterson ◽  
E. Amaya ◽  
T.J. Musci
Keyword(s):  
Protein Interactions ◽  
Translational Control ◽  
Untranslated Region ◽  
Rna Stability ◽  
Immature Oocyte ◽  
Translational Repression ◽  
Meiotic Maturation ◽  
Fgf Receptor ◽  
Cis Acting ◽  
Maternal Mrna

Early frog embryogenesis depends on a maternal pool of mRNA to execute critical intercellular signalling events. FGF receptor-1, which is required for normal development, is stored as a stable, untranslated maternal mRNA transcript in the fully grown immature oocyte, but is translationally activated at meiotic maturation. We have identified a short cis-acting element in the FGF receptor 3′ untranslated region that inhibits translation of synthetic mRNA. This inhibitory element is sufficient to inhibit translation of heterologous reporter mRNA in the immature oocyte without changing RNA stability. Deletion of the poly(A) tract or polyadenylation signal sequences does not affect translational inhibition by this element. At meiotic maturation, we observe the reversal of translational repression mediated by the inhibitory element, mimicking that seen with endogenous maternal FGF receptor mRNA at meiosis. In addition, the activation of synthetic transcripts at maturation does not appear to require poly(A) lengthening. We also show that an oocyte cytoplasmic protein specifically binds the 3′ inhibitory element, suggesting that translational repression of Xenopus FGF receptor-1 maternal mRNA in the oocytes is mediated by RNA-protein interactions. These data describe a mechanism of translational control that appears to be independent of poly(A) changes.

Apontic binds the translational repressor Bruno and is implicated in regulation of oskar mRNA translation

Development ◽  
1999 ◽  
Vol 126 (6) ◽  
pp. 1129-1138 ◽  
Author(s):  
Y.S. Lie ◽  
P.M. Macdonald
Keyword(s):  
Translational Control ◽  
Untranslated Region ◽  
Rna Binding ◽  
Mrna Translation ◽  
Posterior Pole ◽  
Translational Repression ◽  
Yeast Two Hybrid ◽  
Functional Interaction ◽  
Translational Repressor ◽  
Two Hybrid

The product of the oskar gene directs posterior patterning in the Drosophila oocyte, where it must be deployed specifically at the posterior pole. Proper expression relies on the coordinated localization and translational control of the oskar mRNA. Translational repression prior to localization of the transcript is mediated, in part, by the Bruno protein, which binds to discrete sites in the 3′ untranslated region of the oskar mRNA. To begin to understand how Bruno acts in translational repression, we performed a yeast two-hybrid screen to identify Bruno-interacting proteins. One interactor, described here, is the product of the apontic gene. Coimmunoprecipitation experiments lend biochemical support to the idea that Bruno and Apontic proteins physically interact in Drosophila. Genetic experiments using mutants defective in apontic and bruno reveal a functional interaction between these genes. Given this interaction, Apontic is likely to act together with Bruno in translational repression of oskar mRNA. Interestingly, Apontic, like Bruno, is an RNA-binding protein and specifically binds certain regions of the oskar mRNA 3′ untranslated region.

scholarly journals Identification of an active miniature inverted‐repeat transposable element mJ ing in rice

2019 ◽  
Vol 98 (4) ◽  
pp. 639-653 ◽  
Author(s):  
Yanyan Tang ◽  
Xin Ma ◽  
Shuangshuang Zhao ◽  
Wei Xue ◽  
Xu Zheng ◽  
...  
Keyword(s):  
Transposable Element ◽  
Inverted Repeat

Miniature Inverted-Repeat Transposable Element Identification and Genetic Marker Development in Agrostis

Crop Science ◽  
2011 ◽  
Vol 51 (2) ◽  
pp. 854-861 ◽  
Author(s):  
Keenan Amundsen ◽  
David Rotter ◽  
Huaijun Michael Li ◽  
Joachim Messing ◽  
Geunhwa Jung ◽  
...  
Keyword(s):  
Transposable Element ◽  
Genetic Marker ◽  
Inverted Repeat ◽  
Marker Development

scholarly journals Genome-Wide Comparative Analysis of 20 Miniature Inverted-Repeat Transposable Element Families in Brassica rapa and B. oleracea

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94499 ◽  
Author(s):  
Perumal Sampath ◽  
Jayakodi Murukarthick ◽  
Nur Kholilatul Izzah ◽  
Jonghoon Lee ◽  
Hong-Il Choi ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Transposable Element ◽  
Brassica Rapa ◽  
Inverted Repeat ◽  
Genome Wide

Detection of a mariner-like element and a miniature inverted-repeat transposable element (MITE) associated with the heterochromatin from ants of the genus Messor and their possible involvement for satellite DNA evolution

Gene ◽  
2006 ◽  
Vol 371 (2) ◽  
pp. 194-205 ◽  
Author(s):  
Teresa Palomeque ◽  
José Antonio Carrillo ◽  
Martín Muñoz-López ◽  
Pedro Lorite
Keyword(s):  
Transposable Element ◽  
Satellite Dna ◽  
Inverted Repeat ◽  
Dna Evolution ◽  
Satellite Dna Evolution

How do microRNAs regulate gene expression?

2008 ◽  
Vol 36 (6) ◽  
pp. 1224-1231 ◽  
Author(s):  
Ian G. Cannell ◽  
Yi Wen Kong ◽  
Martin Bushell
Keyword(s):  
Gene Expression ◽  
Untranslated Region ◽  
Protein Production ◽  
Translational Repression ◽  
Regulate Gene Expression ◽  
Target Mrna ◽  
Target Mrnas ◽  
Non Coding Rnas ◽  
Regulate Gene

miRNAs (microRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally. They generally bind to the 3′-UTR (untranslated region) of their target mRNAs and repress protein production by destabilizing the mRNA and translational silencing. The exact mechanism of miRNA-mediated translational repression is yet to be fully determined, but recent data from our laboratory have shown that the stage of translation which is inhibited by miRNAs is dependent upon the promoter used for transcribing the target mRNA. This review focuses on understanding how miRNA repression is operating in light of these findings and the questions that still remain.

A conserved 90 nucleotide element mediates translational repression of nanos RNA

Development ◽  
1996 ◽  
Vol 122 (9) ◽  
pp. 2791-2800 ◽  
Author(s):  
E.R. Gavis ◽  
L. Lunsford ◽  
S.E. Bergsten ◽  
R. Lehmann
Keyword(s):  
Translational Control ◽  
Untranslated Region ◽  
Translational Regulation ◽  
Critical Role ◽  
Rna Localization ◽  
Translational Repression ◽  
Regulatory Function ◽  
Control Element ◽  
Cis Acting ◽  
Localization Signal

Correct formation of the Drosophila body plan requires restriction of nanos activity to the posterior of the embryo. Spatial regulation of nanos is achieved by a combination of RNA localization and localization-dependent translation such that only posteriorly localized nanos RNA is translated. Cis-acting sequences that mediate both RNA localization and translational regulation lie within the nanos 3′ untranslated region. We have identified a discrete translational control element within the nanos 3′ untranslated region that acts independently of the localization signal to mediate translational repression of unlocalized nanos RNA. Both the translational regulatory function of the nanos 3′UTR and the sequence of the translational control element are conserved between D. melanogaster and D. virilis. Furthermore, we show that the RNA helicase Vasa, which is required for nanos RNA localization, also plays a critical role in promoting nanos translation. Our results specifically exclude models for translational regulation of nanos that rely on changes in polyadenylation.

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