scholarly journals Detection and quantification of viable airborne bacteria and fungi using solid-phase cytometry

2009 ◽  
Vol 4 (2) ◽  
pp. 224-231 ◽  
Author(s):  
Lies M E Vanhee ◽  
Hans J Nelis ◽  
Tom Coenye
Keyword(s):  
Solid Phase ◽  
Airborne Bacteria ◽  
Bacteria And Fungi ◽  
Detection And Quantification

scholarly journals Enumeration of airborne bacteria and fungi using solid phase cytometry

2008 ◽  
Vol 72 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Lies M.E. Vanhee ◽  
Hans J. Nelis ◽  
Tom Coenye
Keyword(s):  
Solid Phase ◽  
Airborne Bacteria ◽  
Bacteria And Fungi

Assessment of the indoor air quality based on airborne bacteria and fungi measurements in a public school of Istanbul

2020 ◽  
Vol 13 (24) ◽  
Author(s):  
Nuket Sivri ◽  
Ahmet Ozgur Dogru ◽  
Arzu Funda Bagcigil ◽  
Kemal Metiner ◽  
Dursun Zafer Seker
Keyword(s):  
Air Quality ◽  
Public School ◽  
Indoor Air Quality ◽  
Indoor Air ◽  
Airborne Bacteria ◽  
Bacteria And Fungi

Thermal Environmental Interference with Airborne Bacteria and Fungi Levels in Air-Conditioned Offices

2008 ◽  
Vol 17 (2) ◽  
pp. 122-127 ◽  
Author(s):  
L.T. Wong ◽  
K.W. Mui ◽  
P.S. Hui ◽  
W.Y. Chan ◽  
A.K.Y. Law
Keyword(s):  
Airborne Bacteria ◽  
Bacteria And Fungi ◽  
Environmental Interference

scholarly journals Airborne Bacteria and Fungi Concentration in Relation with Bedding Type in Broiler Houses

2019 ◽  
Vol 76 (2) ◽  
pp. 222
Author(s):  
Silvana POPESCU ◽  
Cristin BORDA ◽  
Cristina EL MAHDY ◽  
Eva Andrea LAZAR ◽  
Anamaria BLAGA PETREAN ◽  
...  
Keyword(s):  
Sunflower Seed ◽  
Microbial Contamination ◽  
Production Cycle ◽  
Airborne Bacteria ◽  
Airborne Concentration ◽  
Commercial Farms ◽  
Bacteria And Fungi

The aim of this work was the investigation of the effect of bedding type on the airborne concentration of bacteria and fungi in broiler houses. The study was performed in two commercial farms (A and B) with different bedding (chopped straw in farm A, sunflower seed hulls in farm B). The determination of the bacteria and fungi’s numbers was performed in the first and the sixth weeks of the production cycle. Both the concentration of the bacteria and fungi showed higher values in the farm A compared with the farm B, especially in the latter period of the production cycle. In both farms, significant differences (P < 0.05) were recorded between the two periods of the determinations. The results of this study suggest that using sunflower seed hulls as bedding leads to lower levels of microbial contamination of the air in broiler houses, comparing with the use of chopped straw.

scholarly journals Determination of multi-class herbicides in soil by liquid-solid extraction coupled with headspace solid phase microextraction method

2016 ◽  
Vol 81 (8) ◽  
pp. 923-934 ◽  
Author(s):  
Rada Djurovic-Pejcev ◽  
Tijana Djordjevic ◽  
Vojislava Bursic
Keyword(s):  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Soil Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Optimum Number ◽  
Polydimethyl Siloxane ◽  
Headspace Solid Phase Microextraction ◽  
Relative Standard ◽  
Detection And Quantification

A method is described for simultaneous determination of five herbicides (metribuzin, acetochlor, clomazone, oxyfluorfen and dimethenamid) belonging to different pesticides groups in soil samples. Developed headspace solid phase microextraction method (HS-SPME) in combination with liquid-solid sample preparation (LS) was optimized and applied in the analysis of some agricultural samples. Optimization of microextraction conditions, such as temperature, extraction time and sodium chloride (NaCl) content was perfor-med using 100 ?m polydimethyl-siloxane (PDMS) fiber. The extraction effi-ciencies of methanol, methanol:acetone=1:1 and methanol:acetone:hexane= =2:2:1 and the optimum number of extraction steps during the sample prepa-ration, were tested, as well. Gas chromatography-mass spectrometry (GC-MS) was used for detection and quantification, obtaining relative standard deviation (RSD) below 13%, and recovery values higher than 83% for multiple analyses of soil samples fortified at 30 ?g kg-1 of each herbicide. Limits of detection (LOD) were less than 1.2 ?g kg-1 for all the studied herbicides.

LC-MS Method for the Detection and Quantification of Ursolic Acid and Uvaol Levels in Olive Leaves and Oregano

2020 ◽  
pp. 600
Author(s):  
Naser F. Al-Tannak ◽  
Ladislav Novotny
Keyword(s):  
Biological Activity ◽  
Ursolic Acid ◽  
Mobile Phase ◽  
Solid Phase ◽  
Phase Extraction ◽  
Origanum Vulgare ◽  
Olive Leaves ◽  
Accuracy And Precision ◽  
Acquity Uplc ◽  
Detection And Quantification

Ursolic acid and uvaol belong to pentacyclic triterpenes are both secondary metabolites with significant biological activity. Ursolic acid and uvaol are exist in many natural matrices such as olive leaves, oregano and thymus. Therefore, a new rapid and validated UPLC method was developed to identify and quantify ursolic acid and uvaol in dry olive leaves (Olea europaea L.) planted in Kuwait and dry oregano (Origanum vulgare L.) planted in Palestine and Spain. The separation of ursolic acid and uvaol was achieved by using BEH Phenyl (1.7 μm, 2.1 x 100 mm) analytical column (Waters® Acquity UPLC) and a mobile phase composed of water and acetonitrile (37:63 v/v). Solid-phase extraction cartridges (HyperSep™) was used to extract ursolic acid and uvaol from natural matrices. Ursolic acid and uvaol were detected in all-natural matrices used. The concentrations of ursolic acid and uvaol in olive leaves were 55.10 μg/g ± 0.72 and 314.29 μg/g ± 0.97, respectively. In contrast, ursolic acid and uvaol concentrations in oregano brands were ranging from 25.57 μg/g ± 0.35- 28.70 μg/g ± 0.36 and 19.07 μg/g ± 0.38 - 48.10 μg/g ± 0.62, respectively. In conclusion, the developed method was capable to identify and determine ursolic acid and uvaol in pure powder and natural matrices with good linearity, accuracy and precision.

scholarly journals Carbon and Tin-Based Polyacrylonitrile Hybrid Architecture Solid Phase Microextraction Fiber for the Detection and Quantification of Antibiotic Compounds in Aqueous Environmental Systems

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1670 ◽  
Author(s):  
Sandip Mondal ◽  
Jialing Jiang ◽  
Yin Li ◽  
Gangfeng Ouyang
Keyword(s):  
Solid Phase Microextraction ◽  
Tin Dioxide ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Tap Water ◽  
Coefficient Of Determination ◽  
Lower Amount ◽  
Custom Made ◽  
Relative Standard ◽  
Detection And Quantification

In this study, the detection and quantification of multiple classes of antibiotics in water matrices are proposed using a lab-made solid phase microextraction (SPME) fiber coupled with high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The lab-made fiber was prepared using a graphene oxide (G), carbon nanotubes (C), and tin dioxide (T) composite, namely GCT, with polyacrylonitrile (PAN) as supporting material. The detected antibiotics were enrofloxacin, sulfathiazole, erythromycin, and trimethoprim. The custom-made fiber was found to be superior compared with a commercial C18 fiber. The excellent reproducibility and lower intra-fiber relative standard deviations (RSDs 1.8% to 6.8%) and inter-fiber RSDs (4.5% to 8.8%) made it an ideal candidate for the detection of traces of antibiotics in real environmental samples. The proposed validated method provides a satisfactory limit of detection and good linear ranges with higher (>0.99) coefficient of determination in the aqueous system. Application of the method was made in different real water systems such as river, pond and tap water using the standard spiking method. Excellent sensitivity, reproducibility, lower amount of sample detection and higher recovery was found in a real water sample. Therefore, the extraction method was successfully applied to the detection and quantification of multiple classes of antibiotics in different aqueous systems with satisfactory results.

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