scholarly journals Mitochondrial respiratory uncoupling promotes keratinocyte differentiation and blocks skin carcinogenesis

Oncogene ◽  
2012 ◽  
Vol 31 (44) ◽  
pp. 4725-4731 ◽  
Author(s):  
C U Lago ◽  
S M Nowinski ◽  
J E Rundhaug ◽  
M E Pfeiffer ◽  
K Kiguchi ◽  
...  
Keyword(s):  
Skin Carcinogenesis ◽  
Keratinocyte Differentiation ◽  
Respiratory Uncoupling ◽  
Mitochondrial Respiratory

Abstract 5425: Epidermal respiratory uncoupling blocks skin carcinogenesis by inhibiting Akt.

2013 ◽  
Author(s):  
Sara M. Nowinski ◽  
Joyce E. Rundhaug ◽  
Okkyung Rho ◽  
Cory U. Lago ◽  
Susan M. Fischer ◽  
...  
Keyword(s):  
Skin Carcinogenesis ◽  
Respiratory Uncoupling

scholarly journals The PDZ-Binding Motif of HPV16-E6 Oncoprotein Modulates the Keratinization and Stemness Transcriptional ProfileIn Vivo

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Enoc Mariano Cortés-Malagón ◽  
Carmen Palacios-Reyes ◽  
Sandra Romero-Cordoba ◽  
Daniel Mendoza-Villanueva ◽  
Jaime Escobar-Herrera ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Skin Carcinogenesis ◽  
Early Gene ◽  
Keratinocyte Differentiation ◽  
Binding Motif ◽  
Hpv16 E6 ◽  
Stem Cell Maintenance ◽  
E6 Oncoprotein

Objective. The aim of this work was to compare the early gene expression profiles in the skin of HPV16-E6 transgenic mice regulated by the E6 PDZ-binding motif.Materials and Methods.The global transcriptional profiles in dorsal skin biopsies from K14E6 and K14E6Δ146-151 transgenic mice were compared using microarrays. Relevant genes obtained from the most differentially expressed processes were further examined by RT-qPCR, in situ RT-PCR, Western blot, or immunofluorescence.Results.The transcriptomic landscape of K14E6 versus K14E6Δ146-151 shows that the most affected expression profiles were those related to keratinocyte differentiation, stem cell maintenance, and keratinization. Additionally, downregulation of epidermal stemness markers such as K15 and CD34, as well as the upregulation of cytokeratin 6b, appeared to be dependent on the E6 PDZ-binding motif. Finally, wound healing, a physiological process linked to stemness, is impaired in the K14E6 mice compared to K14E6Δ146-151.Conclusion.The E6 PDZ-binding motif appears to affect stemness and keratinization during early stages of skin carcinogenesis. As E6 plays a significant role in HPV-induced skin carcinogenesis, the K14E6 versus K14E6Δ146-151 transcriptional profile provides a source of valuable data to uncover novel E6 functions in the skin.

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