Structural analysis of microbial poly(ɛ-L-lysine)/poly(acrylic acid) complex by FT-IR, DSC, and solid-state 13C and 15N NMR

AbstractIn this study, urease was immobilized in a polymer network obtained by complexation of poly(1-vinyl imidazole) (PVI) with poly(acrylic acid) (PAA). Preparation of the polymer network was monitored by FT-IR spectroscopy. Scanning electron microscopy (SEM) revealed that enzyme immobilization had a strong effect on film morphology. Proton conductivity of the PVI/PAA network was measured via impedance spectroscopy under humidified conditions. Values of the Michaelis-Menten constant (K M) for immobilized urease were higher than for the free enzyme, indicating a decreased affinity of the enzyme to its substrate. The basic characteristics (pHopt, pHstability, T opt, T stability, reusability, and storage stability) of immobilized urease were determined. The results show that the PAA/PVI polymer network is suitable for enzyme immobilization.

Download Full-text

Dual-Charge Nanofiber Mats Made of Chitosan(CS)/Poly(Vinyl Alcohol) (PVA) and Poly-(Acrylic Acid-Co-Maleic Acid) (PAMA)/PVA

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.819.145 ◽

2019 ◽

Vol 819 ◽

pp. 145-150

Author(s):

Thapakorn Chareonying ◽

Junnasir M. Sakilan ◽

Theerasak Rojanarata ◽

Prasopchai Patrojanasophon ◽

Prasert Akkaramongkolporn ◽

...

Keyword(s):

Acrylic Acid ◽

Maleic Acid ◽

Vinyl Alcohol ◽

Electrospinning Process ◽

Poly Vinyl Alcohol ◽

Water Capacity ◽

Ft Ir ◽

Nanofiber Mats ◽

Positively Charged ◽

Poly Acrylic Acid

Nanofibers have been widely used for tissue engineering. Using charged polymers for the preparation of nanofibers can be useful for the loading of substances or macromolecules. Dual charge nanofiber mats are expected to be able to immobilize both positively charged and negatively charged substances in one versatile nanofiber mat. The purpose of this study was to prepare and characterize dual-charge nanofibers generated from poly (vinyl alcohol) (PVA)/poly-(acrylic acid-co-maleic acid) (PAMA) and chitosan (CS)/PVA. The polymer solutions of PAMA/PVA (1:1.63 w/w) and CS/PVA (1:2.33 w/w) were electrospun to form the nanofibers using dual-jet electrospinning process. The obtained dual-charge nanofibers were thermally crosslinked by leaving the nanofibers in the oven at 110-130 °C for 0.5, 1, 3, 5 h. The appearance of the nanofiber mat was characterized by a scanning electron microscope (SEM), and the diameter of nanofibers were determined by an image analysis software (J-micro vision®). The percentage water insolubilization and FT-IR spectra were also determined. The dual-size nanofiber mats with smooth and bead-free fibers were obtained. The diameter of the PAMA/PVA and CS/PVA fibers was 574.54 ± 142.98 nm and 225.69 ± 41.92 nm, respectively. The desirable temperature and time for the crosslink of the dual-charge nanofiber mats was 130 °C for 1 h which could provide a high insolubilization with water capacity of 93.22 ± 2.23%.

Download Full-text

Structural Analysis of Polyamide Fibers by Solid State 15N NMR.

KOBUNSHI RONBUNSHU ◽

10.1295/koron.51.47 ◽

1994 ◽

Vol 51 (1) ◽

pp. 47-51 ◽

Cited By ~ 3

Author(s):

Joo-Hong YEO ◽

Makoto DEMURA ◽

Takehito KONAKAZAWA ◽

Tetsuo ASAKURA ◽

Takuro ITOH ◽

...

Keyword(s):

Solid State ◽

Structural Analysis ◽

15N Nmr ◽

Polyamide Fibers

Download Full-text

Miscibility of Poly(vinyl alcohol)/Poly(methacrylic acid) and Poly(vinyl alcohol/Poly(acrylic acid) Systems II. High-Resolution Solid-State CP/MAS 13C NMR Studies

Polymer Journal ◽

10.1295/polymj.23.87 ◽

1991 ◽

Vol 23 (2) ◽

pp. 87-93 ◽

Cited By ~ 27

Author(s):

Xiaoqing Zhang ◽

K Takegoshi ◽

Kunio Hikichi

Keyword(s):

Solid State ◽

High Resolution ◽

Acrylic Acid ◽

13C Nmr ◽

Methacrylic Acid ◽

Vinyl Alcohol ◽

Poly Vinyl Alcohol ◽

Nmr Studies ◽

Cp Mas 13C Nmr ◽

Poly Acrylic Acid

Download Full-text

Synthesis and Characteristics of Interpenetrating Polymer Network Hydrogels Based on Konjac Glucomannan with Different Molecular Weights and Poly(acrylic acid)

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.393-395.1004 ◽

2011 ◽

Vol 393-395 ◽

pp. 1004-1007

Author(s):

Xue Yao ◽

Xue Gang Luo ◽

Ben Chao Han

Keyword(s):

Acrylic Acid ◽

Enzymatic Degradation ◽

Drug Carrier ◽

Polymer Network ◽

Konjac Glucomannan ◽

Interpenetrating Polymer Network ◽

Swelling Ratio ◽

Molecular Weights ◽

Ft Ir ◽

Poly Acrylic Acid

Konjac glucomannan with different molecular weights/poly(acrylic acid) hydrogels were prepared in this paper. The structure of the IPN hydrogels was characterized by FT-IR and SEM. The swelling ratio of these hydrogels showed they had pH-sensitive properties and the enzymatic degradation tests showed the hydrogels retain the enzymatic degradation character of KGM. Furthermore, hydrogel composed of native KGM degraded sharply in enzymatic degradation test and it had bigger swelling ratio and weight loss ratio than those hydrogels which composed of lower molecular weights KGM. Therefore, hydrogels composed of lower molecular weight might release drug more stable when they were used as drug carrier.

Download Full-text