Linking genetic, morphological, and behavioural divergence between inland island and mainland deer mice

Heredity ◽  
2021 ◽  
Author(s):  
Joshua M. Miller ◽  
Dany Garant ◽  
Charles Perrier ◽  
Tristan Juette ◽  
Joël W. Jameson ◽  
...  
Keyword(s):  
Deer Mice

An Ultrastructural Comparison of Hepatocytes from ADH+ and ADH−Peromyscus Maniculatus

1996 ◽  
Vol 54 ◽  
pp. 30-31
Author(s):  
J. T. Ellzey ◽  
D. Borunda ◽  
B. P. Stewart
Keyword(s):  
South Carolina ◽  
Adult Male ◽  
Uranyl Acetate ◽  
Model System ◽  
Deer Mice ◽  
En Bloc ◽  
Genetic Stock ◽  
Hepes Buffer ◽  
Transmission Electron ◽  
Color Scanner

Genetically alcohol deficient deer mice (ADHN/ADHN) (obtained from the Peromyscus Genetic Stock Center, Univ. of South Carolina) lack hepatic cytosolic alcohol dehydrogenase. In order to determine if these deer mice would provide a model system for an ultrastructural study of the effects of ethanol on hepatocyte organelles, 75 micrographs of ADH+ adult male deer mice (n=5) were compared with 75 micrographs of ADH− adult male deer mice (n=5). A morphometric analysis of mitochondrial and peroxisomal parameters was undertaken.The livers were perfused with 0.1M HEPES buffer followed by 0.25% glutaraldehyde and 2% sucrose in 0.1M HEPES buffer (4C), removed, weighed and fixed by immersion in 2.5% glutaraldehyde in 0.1M HEPES buffer, pH 7.4, followed by a 3,3’ diaminobenzidine (DAB) incubation, postfixation with 2% OsO4, en bloc staining with 1% uranyl acetate in 0.025M maleate-NaOH buffer, dehydrated, embedded in Poly/Bed 812-BDMA epon resin, sectioned and poststained with uranyl acetate and lead citrate. Photographs were taken on a Zeiss EM-10 transmission electron microscope, scanned with a Howtek personal color scanner, analyzed with OPTIMAS 4.02 software on a Gateway2000 4DX2-66V personal computer and stored in Excel 4.0.

Identification of the pulmonary syndrome hantavirus by direct and colloidal gold immune Electron Microscopy

1994 ◽  
Vol 52 ◽  
pp. 274-275
Author(s):  
C. D. Humphrey ◽  
C.S. Goldsmith ◽  
L. Elliott ◽  
S.R. Zaki
Keyword(s):  
Electron Microscopy ◽  
United States ◽  
Monoclonal Antibody ◽  
Colloidal Gold ◽  
Phosphotungstic Acid ◽  
Uranyl Acetate ◽  
Hantavirus Pulmonary Syndrome ◽  
Deer Mice ◽  
Immune Electron Microscopy ◽  
Negative Stain

An outbreak of unexplained acute pulmonary syndrome with high fatality was recognized in the spring of 1993 in the southwestern United States. The cause of the illness was quickly identified serologically and genetically as a hantavirus and the disease was named hantavirus pulmonary syndrome (HPS). Recently, the virus was isolated from deer mice which had been trapped near the homes of HPS patients, and cultivated in Vero E6 cells. We identified the cultivated virus by negative-stain direct and colloidal gold immune electron microscopy (EM).Virus was extracted, clarified, and concentrated from unfixed and 0.25% glutaraldehyde fixed supernatant fluids of infected Vero E6 cells by a procedure described previously. Concentrated virus suspensions tested by direct EM were applied to glow-discharge treated formvar-carbon filmed grids, blotted, and stained with 0.5% uranyl acetate (UA) or with 2% phosphotungstic acid (PTA) pH 6.5. Virus suspensions for immune colloidal gold identification were adsorbed similarly to filmed grids but incubated for 1 hr on drops of 1:50 diluted monoclonal antibody to Prospect Hill virus nucleoprotein or with 1:50 diluted sera from HPS virus infected deer mice.

scholarly journals Dehydrogenase-dependent Ethanol Metabolism in Deer Mice (Peromyscus maniculatus) Lacking Cytosolic Alcohol Dehydrogenase

1989 ◽  
Vol 264 (10) ◽  
pp. 5593-5597
Author(s):  
C Norsten ◽  
T Cronholm ◽  
G Ekström ◽  
J A Handler ◽  
R G Thurman ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Peromyscus Maniculatus ◽  
Ethanol Metabolism ◽  
Deer Mice

scholarly journals SARS-CoV-2 infection and transmission in the North American deer mouse

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Bryan D. Griffin ◽  
Mable Chan ◽  
Nikesh Tailor ◽  
Emelissa J. Mendoza ◽  
Anders Leung ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
North American ◽  
Direct Contact ◽  
Lower Respiratory Tract ◽  
Infectious Virus ◽  
Deer Mouse ◽  
Deer Mice ◽  
The North ◽  
Wild Deer ◽  
Theoretical Risk

AbstractWidespread circulation of SARS-CoV-2 in humans raises the theoretical risk of reverse zoonosis events with wildlife, reintroductions of SARS-CoV-2 into permissive nondomesticated animals. Here we report that North American deer mice (Peromyscus maniculatus) are susceptible to SARS-CoV-2 infection following intranasal exposure to a human isolate, resulting in viral replication in the upper and lower respiratory tract with little or no signs of disease. Further, shed infectious virus is detectable in nasal washes, oropharyngeal and rectal swabs, and viral RNA is detectable in feces and occasionally urine. We further show that deer mice are capable of transmitting SARS-CoV-2 to naïve deer mice through direct contact. The extent to which these observations may translate to wild deer mouse populations remains unclear, and the risk of reverse zoonosis and/or the potential for the establishment of Peromyscus rodents as a North American reservoir for SARS-CoV-2 remains unknown.

Stereotypy and spontaneous alternation in deer mice and its response to anti‐adenosinergic intervention

2021 ◽  
Author(s):  
Geoffrey Browuer ◽  
Jaco Engelbrecht ◽  
Daniel C. Mograbi ◽  
Lesetja Legoabe ◽  
Stephan F. Steyn ◽  
...  
Keyword(s):  
Spontaneous Alternation ◽  
Deer Mice

scholarly journals Resilience, plasticity and robustness in gene expression during aging in the brain of outbred deer mice

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
E Soltanmohammadi ◽  
Y Zhang ◽  
I Chatzistamou ◽  
H. Kiaris
Keyword(s):  
Gene Expression ◽  
Cholesterol Metabolism ◽  
Expression Profiles ◽  
Deer Mice ◽  
Abrupt Changes ◽  
Transcriptional Changes ◽  
Thrombospondin 4 ◽  
Whole Transcriptome ◽  
The Brain ◽  
Shed Light

Abstract Background Genes that belong to the same network are frequently co-expressed, but collectively, how the coordination of the whole transcriptome is perturbed during aging remains unclear. To explore this, we calculated the correlation of each gene in the transcriptome with every other, in the brain of young and older outbred deer mice (P. leucopus and P. maniculatus). Results In about 25 % of the genes, coordination was inversed during aging. Gene Ontology analysis in both species, for the genes that exhibited inverse transcriptomic coordination during aging pointed to alterations in the perception of smell, a known impairment occurring during aging. In P. leucopus, alterations in genes related to cholesterol metabolism were also identified. Among the genes that exhibited the most pronounced inversion in their coordination profiles during aging was THBS4, that encodes for thrombospondin-4, a protein that was recently identified as rejuvenation factor in mice. Relatively to its breadth, abolishment of coordination was more prominent in the long-living P. leucopus than in P. maniculatus but in the latter, the intensity of de-coordination was higher. Conclusions There sults suggest that aging is associated with more stringent retention of expression profiles for some genes and more abrupt changes in others, while more subtle but widespread changes in gene expression appear protective. Our findings shed light in the mode of the transcriptional changes occurring in the brain during aging and suggest that strategies aiming to broader but more modest changes in gene expression may be preferrable to correct aging-associated deregulation in gene expression.

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