scholarly journals Downregulated expression of S2-RNase attenuates self-incompatibility in “Guiyou No. 1” pummelo

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jianbing Hu ◽  
Qiang Xu ◽  
Chenchen Liu ◽  
Binghao Liu ◽  
Chongling Deng ◽  
...  

AbstractSelf-incompatibility (SI) substantially restricts the yield and quality of citrus. Therefore, breeding and analyzing self-compatible germplasm is of great theoretical and practical significance for citrus. Here, we focus on the mechanism of a self-compatibility mutation in ‘Guiyou No. 1’ pummelo (Citrus maxima), which is a spontaneous mutant of ‘Shatian’ pummelo (Citrus maxima, self-incompatibility). The rate of fruit set and the growth of pollen tubes in the pistil confirmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of ‘Guiyou No. 1’. Segregation ratios of the S genotype in F1 progeny, expression analysis, and western blotting validated that the reduced levels of S2-RNase mRNA contribute to the loss of SI in ‘Guiyou No. 1’. Furthermore, we report a phased assembly of the ‘Guiyou No. 1’ pummelo genome and obtained two complete and well-annotated S haplotypes. Coupled with an analysis of SV variations, methylation levels, and gene expression, we identified a candidate gene (CgHB40), that may influence the regulation of the S2-RNase promoter. Our data provide evidence that a mutation that affects the pistil led to the loss of SI in ‘Guiyou No. 1’ by influencing a poorly understood mechanism that affects transcriptional regulation. This work significantly advances our understanding of the genetic basis of the SI system in citrus and provides information on the regulation of S-RNase genes.

Author(s):  
MNA Chowdhury ◽  
MA Rahim

Effect of different fertilizers on the control of anthracnose disease, yield and quality of mango cv Amrapali was investigated. Application of combined fertilizers gave the highest fruit set, fruit retention and fresh fruits, therefore, produced the highest yield per plant and per hectare. The highest number of healthy fruits was found from 2nd year crop and the lowest was obtained from 1st year. The highest (8.47 t/ha) yield was obtained from 2nd year and the lowest (4.58 t/ha) was obtained from 1st year crop. Among the treatments, combined application of NPK, ZnSo4, Gypsum and Cowdung gave the highest (50.67) number of healthy fruits and the lowest (16) was obtained from untreated plant. The highest (12.41 t/ha) yield was obtained from combined application of NPK, ZnSo4, Gypsum and Cowdung and the lowest (3.88 t/ha) was obtained from untreated plant. In respect of combined effect, the highest (15.22 t/ha) yield was obtained from 2nd year x T9 treated plant and the lowest (2.95 t/ha) was found in untreated 1st year plant. Key words: Mango, fertilizers, anthracnose, yield. DOI = 10.3329/jard.v5i1.1461 J Agric Rural Dev 5(1&2), 70-78, June 2007


2016 ◽  
Vol 41 (3) ◽  
pp. 419-432
Author(s):  
MS Alam ◽  
N Islam ◽  
S Ahmad ◽  
MI Hossen ◽  
MR Islam

The study was carried out during summer of 2012 with BARI hybrid tomato 4, planted in the Olericulture farm of Bangladesh Agriculture Research Institute, Joydebpur, Gazipur, Bangladesh to find out the response of plants to some staking and pruning treatments on yield, fruit quality and cost of production. A two factor experiment consisting of three staking methods and four level of pruning, laid out in complete block design with three repetitions. Plants were staked on inverted „V? shaped staking, high platform and string. The plants were pruned to two stem, three stem, four stem and no pruning as control. Results showed that significantly the highest total number of fruits per plant (37.1), marketable fruits per plant (33.7), yield per plant (1.68 kg) and total yield (44.6 t/ha) were produced by the plants having the treatment string staking with four stem. The highest fruit set (43.50%) was found in the plants staking with string having three stems. Plants grown on string staking allowing two stem gave the maximum length (4.71 cm), diameter (4.83 cm) and weight (53.4g) of single fruit as well as maximum fruit firmness (3.43 kg-f cm-2). From the economic point of view, it was apparent that summer tomato produced by string staking with four stem pruning exhibited better performance compared to other treatment combinations in relation to net return and BCR (2.10).Bangladesh J. Agril. Res. 41(3): 419-432, September 2016


2005 ◽  
Vol 56 (5) ◽  
pp. 497
Author(s):  
K. Usha ◽  
D. Kashyap ◽  
B. Singh

Gibberellins have been used to improve quality of grapes in several cases. However, gibberellic acid (GA) alone is not enough to improve the quality in Perlette. A field experiment was conducted on 3-year-old grape cv. Perlette trained on pergolas, to investigate the effect of bunch dipping with GA3 and N6-benzyladenine (BA) on reducing the problem of shot berries and improving the productivity of grapevines. Our observations proved that the stage of application of GA3 and BA is crucial to avoiding seed formation in seedless varieties such as Perlette for table grapes. The results showed that BA at low (10 µL/L) or high concentration (30 µL/L) at 50% of full bloom caused seed development in the seedless grape cv. Perlette, whereas no seed was formed when BA was used later at the fruit set stage. Based on our studies, we found that bunch dipping with 30 µL/L GA3 (at 50% of full bloom) and 10µL/L BA (at pea size) significantly reduced the problem of shot berries, and improved the yield and quality in grape cv. Perlette.


HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 430D-430
Author(s):  
Dena C. Fiacchino ◽  
S. Alan Walters

Due to the lack of viable pollen produced in seedless (triploid) watermelons, fruit set in seedless watermelons requires a standard seed (diploid) producing cultivar (hereafter referred to as a pollinizer) to be interplanted as a source of pollen. It is recommended that one row of pollinizer be planted for every two rows of seedless watermelon. There is little to no information available to growers comparing the effects of pollinizers on seedless watermelon yield and quality. We conducted a study to evaluate the effectiveness of three seeded pollinizers (`Crimson Sweet', `Fiesta', and `Royal Sweet') on yield and quality of four seedless watermelon hybrids (`Abbott and Cobb 5244', `Crimson Trio', `Millionaire', and `Tri-X 313'). `Royal Sweet' as a pollinizer tended to produce higher yields of small (<3.6 kg) and medium-sized seedless watermelons (3.6-7.3 kg) per acre compared to `Crimson Sweet' and `Fiesta'. `Crimson Sweet' produced a greater number of large (>7.3 kg) seedless watermelons compared to `Fiesta' and `Royal Sweet'. However, the number of marketable melons (>3.6 kg) did not differ among the three pollinizers evaluated. Two quality measurements taken (hollow heart and soluble sugars) were not influenced by the choice of pollinizer.


2021 ◽  
pp. 203-213
Author(s):  
Rosemary J. du Preez

Abstract This chapter describes the guava fruit as well as the fruit set, growth, drop, harvesting, yield and quality (total soluble solids, total acids and ascorbic acid). Understanding the phenological cycle of guava and the effect of management practices on yield and fruit quality allows management practices to be modified to develop strategies which should lead to productivity gains for different pruning or chemical application times. As the effects of various treatments and times of application on yield and fruit quality for different cultivars and climatic zones are known, it is possible to decide on the optimum time to apply treatments knowing what the quality of the fruit will be, the time that the fruit will be harvested, expected yield and the critical times to apply management practices.


2020 ◽  
Vol 9 (1) ◽  
pp. 88-91
Author(s):  
SA Banday ◽  
Javed Ahmad Bhat ◽  
F A Ahanger ◽  
M M Mir ◽  
Umar Iqbal ◽  
...  

1979 ◽  
Vol 59 (4) ◽  
pp. 1085-1091 ◽  
Author(s):  
JOHN O’SULLIVAN

The effect of rate of N fertilizer and irrigation on yield and quality of peppers (Capsicum annum L.) was studied oyer a 5-yr period from 1974 to 1978 and data for 4 yr are presented. Irrigation significantly increased yield only in 2 yr (1974 and 1978) when rainfall was below normal. Yield differences due to irrigation were attributed to increased fruit numbers per plant (increased fruit set), increased fruit size, increased wall thickness and a reduced number of non-marketable fruit, due to a reduction in the incidence of sun scald and blossom-end rot. The response to N was variable. Rates of N greater than 70 kg/ha did not in general result in increased yields. Higher rates of N reduced yields in 1978, due to the adverse effect of high N on fruit set, resulting in reduced fruit numbers per plant. Rate of N had no effect on fruit size or wall thickness, but high N increased the incidence of blossom-end rot and delayed maturity in some seasons. The interaction between irrigation and N was not significant. Petiole NO3-N content at early fruit set reflected the rate of N applied. When the concentration of NO3-N in the tissue dropped below 0.4%, yields were significantly reduced.


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