Author Correction: Bacterial adaptation is constrained in complex communities

Hfq is a bacterial regulator with key roles in gene expression. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, thanks to its binding to small regulatory noncoding RNAs. This property is of primary importance for bacterial adaptation and survival in hosts. Small RNAs and Hfq are, for instance, involved in the response to antibiotics. Previous work has shown that the E. coli Hfq C-terminal region (Hfq-CTR) self-assembles into an amyloid structure. It was also demonstrated that the green tea compound EpiGallo Catechin Gallate (EGCG) binds to Hfq-CTR amyloid fibrils and remodels them into nonamyloid structures. Thus, compounds that target the amyloid region of Hfq may be used as antibacterial agents. Here, we show that another compound that inhibits amyloid formation, apomorphine, may also serve as a new antibacterial. Our results provide an alternative in order to repurpose apomorphine, commonly used in the treatment of Parkinson’s disease, as an antibiotic to block bacterial adaptation to treat infections.

Download Full-text

Bioleaching of Pyrrhotite with Bacterial Adaptation and Biological Oxidation for Iron Recovery

Metals ◽

10.3390/met11020295 ◽

2021 ◽

Vol 11 (2) ◽

pp. 295

Author(s):

Bong-Ju Kim ◽

Yong-Kwon Koh ◽

Jang-Soon Kwon

Keyword(s):

Mine Drainage ◽

Mine Waste ◽

Point Of Zero Charge ◽

Biological Oxidation ◽

Indigenous Bacteria ◽

Bacterial Adaptation ◽

Valuable Metals ◽

Initial Ph ◽

Dominant Member ◽

Corrosion Pits

The microbially mediated recovery of valuable metals contained in mining waste presents an economical alternative to conventional hydrometallurgical processes. In order to investigate the effect of bacterial adaptation and biological oxidation on bioleaching, the microbially mediated bioleaching of a pyrrhotite sample from mine waste, with indigenous bacteria existing in acid mine drainage, was studied. The indigenous bacteria were sub-cultured repeatedly for iron adaptation, and Acidithiobacillus ferrooxidans was identified as the dominant member of the microbial consortium. The point of zero charge (PZC) of pyrrhotite sampled from mine waste was determined as 3.0. The performance of bioleaching by contact and non-contact biological oxidation was compared by conducting bioleaching under different initial pH (pHini) conditions (2.8 and 3.2). Negatively charged bacteria could be attached onto the pyrrhotite, which has a positive surface charge at lower pHini (2.8) than the PZC (3.0). Bacteria attachment and corrosion pits on the surface of the pyrrhotite residues were observed at pHini of 2.8. Under bacteria-adapted conditions, the leaching concentration of Fe (44.2 mg/L) at pHini of 2.8 was 2.1 times greater than that (21.3 mg/L) at pHini of 3.2. Under non-adapted bacteria conditions, the extent of Fe leaching was not significantly different between the pHini of 2.8 and 3.2. This could be attributed to the fact that the adapted bacteria could more easily attach onto the pyrrhotite surfaces at pHini 2.8, allowing contact biological oxidation during the bioleaching experiments. We demonstrate here that the bioleaching of pyrrhotite could increase Fe recovery through bacterial adaptation and contact biological oxidation.

Download Full-text

Amoeba host-Legionellasynchronization of amino acid auxotrophy and its role in bacterial adaptation and pathogenic evolution

Environmental Microbiology ◽

10.1111/1462-2920.12290 ◽

2013 ◽

Vol 16 (2) ◽

pp. 350-358 ◽

Cited By ~ 33

Author(s):

Christopher T. D. Price ◽

Ashley M. Richards ◽

Juanita E. Von Dwingelo ◽

Hala A. Samara ◽

Yousef Abu Kwaik

Keyword(s):

Amino Acid ◽

Bacterial Adaptation ◽

Pathogenic Evolution

Download Full-text

Genomic Analysis of Citrobacter Portucalensis Sb-2 Identifies a Phage Predation Driven Metalloid Resistance

10.21203/rs.3.rs-470342/v1 ◽

2021 ◽

Author(s):

Yanshuang Yu ◽

Zhenchen Xie ◽

Jigang Yang ◽

Jinxuan Liang ◽

YuanPing Li ◽

...

Keyword(s):

Genetic Diversity ◽

Gene Transfer ◽

Horizontal Gene Transfer ◽

Significant Role ◽

Genome Analysis ◽

Extreme Environments ◽

Genomic Analysis ◽

Resistant Bacterium ◽

Bacterial Adaptation ◽

Resistance Determinants

Abstract Bacterial adaptation to extreme environments is often mediated by horizontal gene transfer (HGT). At the same time, phage mediated HGT for conferring bacterial arsenite and antimonite resistance has not been documented before. In this study, a highly arsenite and antimonite resistant bacterium, C. portucalensis strain Sb-2, was isolated and subsequent genome analysis showed that putative arsenite and antimonite resistance determinants were flanked or embedded by prophages. We predict these phage-mediated resistances play a significant role in maintaining genetic diversity within the genus of Citrobacter and are responsible for endowing the corresponding resistances to C. portucalensis strain Sb-2.

Download Full-text