Preferential Accumulation of Phospholipid-PEG and Cholesterol-PEG Decorated Gold Nanorods into Human Skin Layers and Their Photothermal-Based Antibacterial Activity

Purpose Staphylococcus aureus (S. aureus), Klebsiella pneumoniae (K. pneumoniae) and Streptococcus pneumoniae (S. pneumoniae) are among the pathogens detected during Hajj pilgrimage known to cause pneumonia. This study aims to evaluate the antibacterial activity of activated carbon cloth (ACC) with Ag+ impregnated with zinc oxide nanoparticles (ZnO NPs) against these pathogens. Design/methodology/approach ZnO NPs were impregnated into ACC-Ag+ via layer-by-layer (LbL) self-assembly. Scanning electron microscope (SEM) was used to observe the fine surface morphological details of the ACC-Ag+-ZnO sheets. Antibacterial activity of the ACC-Ag+-ZnO sheets was evaluated using the disk-diffusion susceptibility assay. Allergy patch test was done to evaluate allergic reactions of the ACC-Ag+-ZnO sheets on human skin. Findings SEM micrographs showed successful impregnation of ZnO NPs into the ACC-Ag+ sheets. Disk-diffusion susceptibility assay results of ACC-Ag+-ZnO sheets against S. aureus, K. pneumoniae and S. pneumoniae showed good antibacterial activity; with 1.82 ± 0.13 mm zone of inhibition for S. pneumoniae, at a ZnO concentration of 0.78 mg mL-1. No signs of human skin irritation were observed throughout the allergy patch test. Originality/value Results indicate that ACC-Ag+-ZnO sheets could potentially be embedded within surgical face masks (pilgrims’ preferred) to reduce the risks involved with the transmission of respiratory tract infections during and after mass gatherings (e.g. Hajj/Umrah, Olympics).

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Synchrotron-based X-ray fluorescence study of gold nanorods and skin elements distribution into excised human skin layers

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2018.02.021 ◽

2018 ◽

Vol 165 ◽

pp. 118-126 ◽

Cited By ~ 8

Author(s):

Nouf N. Mahmoud ◽

Messaoud Harfouche ◽

Alaaldin M. Alkilany ◽

Amal G. Al-Bakri ◽

Rania A. El-Qirem ◽

...

Keyword(s):

Human Skin ◽

Gold Nanorods ◽

Fluorescence Study ◽

X Ray

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Study on the protective effect of an innovative cow milk-based product against some human skin-bacterial pathogens

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale ◽

10.4081/jbr.2018.7426 ◽

2018 ◽

Vol 91 (1) ◽

Author(s):

Carlo Cosentino ◽

Hazem Salaheldin Elshafie ◽

Cristiana Labella ◽

Carmine D'Adamo ◽

Giovanni Pecora ◽

...

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Biological Activity ◽

Chemical Composition ◽

Protective Effect ◽

Human Skin ◽

Bacterial Pathogens ◽

Cow Milk ◽

Inflammatory Factors ◽

Total Counting

There is a growing interest related to the possible use of some components of cow milk that behave as immunomodulators, exert biological activity, and have anti-inflammatory factors. The aims of the study were: i) to investigate the chemical composition and antioxidant activity of Podolian cow milk; ii) to test the efficacy of some hand soaps supplemented with different percentage of cow milk compared to placebo; iii) to evaluate the antibacterial activity of milk added to soap. Antioxidant activity of Podolian milk resulted in mean 97.03% and 52.09% utilizing 2,2’-azinobis 3-ethylbenzthiazoline-6-acid and 2,2-diphenyl-1-picrylhydrazyl methods, respectively. The efficacy test showed that the most effective soap was obtained adding 5% of Podolian milk. For this soap, total counting bacterial test evidenced the highest reduction of bacterial hand contamination assessed by 98%.

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Correlation between ICP-OES and Synchrotron-XRF in Detecting the Penetration of Gold Nanorods into Excised Human Skin Layers

Microscopy and Microanalysis ◽

10.1017/s1431927618014885 ◽

2018 ◽

Vol 24 (S2) ◽

pp. 538-539

Author(s):

Nouf N. Mahmoud ◽

Messaoud Harfouche ◽

Alaaldin M. Alkilany ◽

Amal G. Al-Bakri ◽

Enam A. Khalil

Keyword(s):

Human Skin ◽

Gold Nanorods ◽

Icp Oes

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Antibacterial Activity of Polymer Blend Nanocomposites with the Incorporation of Bentonite and Gold Nanorods

Polymer Science Series B ◽

10.1134/s1560090421050031 ◽

2021 ◽

Vol 63 (5) ◽

pp. 598-605

Author(s):

Sohini Chakraborty ◽

Mekha Mariam Mathew ◽

Remya Simon ◽

Nisha George ◽

Anoop Vadakkekara ◽

...

Keyword(s):

Antibacterial Activity ◽

Polymer Blend ◽

Gold Nanorods ◽

Blend Nanocomposites

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Antibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts

International Journal of Nanomedicine ◽

10.2147/ijn.s145531 ◽

2017 ◽

Vol Volume 12 ◽

pp. 7311-7322 ◽

Cited By ~ 17

Author(s):

Nouf Mahmoud ◽

Alaaldin Alkilany ◽

Enam Khalil ◽

Amal Al-Bakri

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Gold Nanorods ◽

Propionibacterium Acnes

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A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100103978 ◽

1988 ◽

Vol 46 ◽

pp. 382-383

Author(s):

Douglas R. Keene ◽

Robert W. Glanville ◽

Eva Engvall

Keyword(s):

Electron Microscopy ◽

Monoclonal Antibody ◽

Human Skin ◽

Basement Membranes ◽

Primary Antibody ◽

Fat Cells ◽

Secondary Antibody ◽

Type Vi Collagen ◽

Dermal Matrix ◽

Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

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Effect of Acetone and Kerosene on Skin Ultrastructure

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100093717 ◽

1972 ◽

Vol 30 ◽

pp. 92-93

Author(s):

A. P. Lupulescu ◽

H. Pinkus ◽

D. J. Birmingham

Keyword(s):

Electron Microscopy ◽

Human Skin ◽

Osmium Tetroxide ◽

Human Epidermis ◽

Ultrastructural Changes ◽

Chemical Agents ◽

Skin Ultrastructure ◽

Volar Surface

Our laboratory is engaged in the study of the effect of different chemical agents on human skin, using electron microscopy. Previous investigations revealed that topical use of a strong alkali (NaOH 1N) or acid (HCl 1N), induces ultrastructural changes in the upper layers of human epidermis. In the current experiments, acetone and kerosene, which are primarily lipid solvents, were topically used on the volar surface of the forearm of Caucasian and Negro volunteers. Skin specimens were bioptically removed after 90 min. exposure and 72. hours later, fixed in 3% buffered glutaraldehyde, postfixed in 1% phosphate osmium tetroxide, then flat embedded in Epon.

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