scholarly journals Bone marrow adipose tissue does not express UCP1 during development or adrenergic-induced remodeling

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Clarissa S. Craft ◽  
Hero Robles ◽  
Madelyn R. Lorenz ◽  
Eric D. Hilker ◽  
Kristann L. Magee ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adipose Tissue ◽  
Uncoupling Protein ◽  
Enrichment Analysis ◽  
Whole Body ◽  
Mineral Density ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue ◽  
Reporter Mice ◽  
Brown Adipose

AbstractAdipocytes within the skeleton are collectively termed bone marrow adipose tissue (BMAT). BMAT contributes to peripheral and local metabolism, however, its capacity for cell-autonomous expression of uncoupling protein 1 (UCP1), a biomarker of beige and brown adipogenesis, remains unclear. To overcome this, Ucp1-Cre was used to drive diphtheria toxin expression in cells expressing UCP1 (Ucp1Cre+/DTA+). Despite loss of brown adipose tissue, BMAT volume was not reduced in Ucp1Cre+/DTA+ mice. Comparably, in mTmG reporter mice (Ucp1Cre+/mTmG+), Ucp1-Cre expression was absent from BMAT in young (3-weeks) and mature (16-weeks) male and female mice. Further, β3-agonist stimulation failed to induce Ucp1-Cre expression in BMAT. This demonstrates that BMAT adipocytes are not UCP1-expressing beige/brown adipocytes. Thus, to identify novel and emerging roles for BMAT adipocytes in skeletal and whole-body homeostasis, we performed gene enrichment analysis of microarray data from adipose tissues of adult rabbits. Pathway analysis revealed genetic evidence for differences in BMAT including insulin resistance, decreased fatty acid metabolism, and enhanced contributions to local processes including bone mineral density through candidate genes such as osteopontin. In sum, this supports a paradigm by which BMAT adipocytes are a unique subpopulation that is specialized to support cells within the skeletal and hematopoietic niche.

scholarly journals Human Bone Marrow Adipose Tissue is a Metabolically Active and Insulin-Sensitive Distinct Fat Depot

2020 ◽  
Vol 105 (7) ◽  
pp. 2300-2310 ◽  
Author(s):  
Tam T Pham ◽  
Kaisa K Ivaska ◽  
Jarna C Hannukainen ◽  
Kirsi A Virtanen ◽  
Martin E Lidell ◽  
...  
Keyword(s):  
Bariatric Surgery ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Insulin Sensitivity ◽  
Whole Body ◽  
Morbidly Obese ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue ◽  
Obese Subjects ◽  
Fat Depot

Abstract Context Bone marrow (BM) in adult long bones is rich in adipose tissue, but the functions of BM adipocytes are largely unknown. We set out to elucidate the metabolic and molecular characteristics of BM adipose tissue (BMAT) in humans. Objective Our aim was to determine if BMAT is an insulin-sensitive tissue, and whether the insulin sensitivity is altered in obesity or type 2 diabetes (T2DM). Design This was a cross-sectional and longitudinal study. Setting The study was conducted in a clinical research center. Patients or Other Participants Bone marrow adipose tissue glucose uptake (GU) was assessed in 23 morbidly obese subjects (9 with T2DM) and 9 healthy controls with normal body weight. In addition, GU was assessed in another 11 controls during cold exposure. Bone marrow adipose tissue samples for molecular analyses were collected from non-DM patients undergoing knee arthroplasty. Intervention(s) Obese subjects were assessed before and 6 months after bariatric surgery and controls at 1 time point. Main Outcome Measure We used positron emission tomography imaging with 2-[18F]fluoro-2-deoxy-D-glucose tracer to characterize GU in femoral and vertebral BMAT. Bone marrow adipose tissue molecular profile was assessed using quantitative RT-PCR. Results Insulin enhances GU in human BMAT. Femoral BMAT insulin sensitivity was impaired in obese patients with T2DM compared to controls, but it improved after bariatric surgery. Furthermore, gene expression analysis revealed that BMAT was distinct from brown and white adipose tissue. Conclusions Bone marrow adipose tissue is a metabolically active, insulin-sensitive and molecularly distinct fat depot that may play a role in whole body energy metabolism.

scholarly journals Ethnic and sex differences in bone marrow adipose tissue and bone mineral density relationship

2011 ◽  
Vol 23 (9) ◽  
pp. 2293-2301 ◽  
Author(s):  
W. Shen ◽  
J. Chen ◽  
M. Gantz ◽  
M. Punyanitya ◽  
S. B. Heymsfield ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Bone Marrow ◽  
Sex Differences ◽  
Adipose Tissue ◽  
Bone Mineral ◽  
Mineral Density ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue ◽  
Density Relationship

KCTD10 regulates brown adipose tissue thermogenesis and metabolic function via Notch Signaling

2021 ◽  
Author(s):  
Mingsheng Ye ◽  
Liping Luo ◽  
Qi Guo ◽  
Guanghua Lei ◽  
Chao Zeng ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Notch Signaling ◽  
Molecular Mechanisms ◽  
Uncoupling Protein ◽  
Notch Signaling Pathway ◽  
Whole Body ◽  
Brown Adipocyte ◽  
Metabolic Function ◽  
Brown Adipose

Brown adipose tissue (BAT) is emerging as a target to beat obesity through the dissipation of chemical energy to heat. However, the molecular mechanisms of brown adipocyte thermogenesis remain to be further elucidated. Here, we show that KCTD10, a member of the polymerase delta-interacting protein 1 (PDIP1) family, was reduced in BAT by cold stress and a β3 adrenoceptor agonist. Moreover, KCTD10 level increased in the BAT of obese mice, and KCTD10 overexpression attenuates uncoupling protein 1 (UCP1) expression in primary brown adipocytes. BAT-specific KCTD10 knockdown mice had increased thermogenesis and cold tolerance protecting from high fat diet (HFD)-induced obesity. Conversely, overexpression of KCTD10 in BAT caused reduced thermogenesis, cold intolerance, and obesity. Mechanistically, inhibiting Notch signaling restored the KCTD10 overexpression suppressed thermogenesis. Our study presents that KCTD10 serves as an upstream regulator of notch signaling pathway to regulate BAT thermogenesis and whole-body metabolic function.

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