Synergistic Antibacterial Activity and Wound Healing Properties of Selenium-Chitosan-Mupirocin Nanohybrid System: An in Vivo Study on Rat Diabetic Staphylococcus aureus Wound Infection Model

Staphylococcus aureus is the most common cause of surgical site infections and its treatment is challenging due to the emergence of multi-drug resistant strains such as methicillin-resistant S. aureus (MRSA). Natural berry-derived compounds have shown antimicrobial potential, e.g., ellagitannins such as sanguiin H-6 and lambertianin C, the main phenolic compounds in Rubus seeds, have shown antimicrobial activity. The aim of this study was to evaluate the effect of sanguiin H-6 and lambertianin C fractionated from cloudberry seeds, on the MRSA growth, and as treatment of a MRSA biofilm development in different growth media in vitro and in vivo by using a murine wound infection model where sanguiin H-6 and lambertianin C were used to prevent the MRSA infection. Sanguiin H-6 and lambertianin C inhibited the in vitro biofilm development and growth of MRSA. Furthermore, sanguiin H-6 showed significant anti-MRSA effect in the in vivo wound model. Our study shows the possible use of sanguiin H-6 as a preventive measure in surgical sites to avoid postoperative infections, whilst lambertianin C showed no anti-MRSA activity.

Download Full-text

Challenges with Wound Infection Models in Drug Development

Current Drug Targets ◽

10.2174/1389450121666200302093312 ◽

2020 ◽

Vol 21 (13) ◽

pp. 1301-1312 ◽

Cited By ~ 1

Author(s):

Sandeep K. Shukla ◽

Ajay K. Sharma ◽

Vanya Gupta ◽

Aman Kalonia ◽

Priyanka Shaw

Keyword(s):

Wound Healing ◽

Wound Infection ◽

Chronic Wound ◽

Wound Care ◽

Infection Model ◽

In Vivo Models ◽

Infection Models

: Wound research is an evolving science trying to unfold the complex untold mechanisms behind the wound healing cascade. In particular, interest is growing regarding the role of microorganisms in both acute and chronic wound healing. Microbial burden plays an important role in the persistence of chronic wounds, ultimately resulting in delayed wound healing. It is therefore important for clinicians to understand the evolution of infection science and its various etiologies. Therefore, to understand the role of bacterial biofilm in chronic wound pathogenesis, various in vitro and in vivo models are required to investigate biofilms in wound-like settings. Infection models should be refined comprising an important signet of biofilms. These models are eminent for translational research to obtain data for designing an improved wound care formulation. However, all the existing models possess limitations and do not fit properly in the model frame for developing wound care agents. Among various impediments, one of the major drawbacks of such models is that the wound they possess does not mimic the wound a human develops. Therefore, a novel wound infection model is required which can imitate the human wounds. : This review article mainly discusses various in vitro and in vivo models showing microbial colonization, their advantages and challenges. Apart from these models, there are also present ex vivo wound infection models, but this review mainly focused on various in vitro and in vivo models available for studying wound infection in controlled conditions. This information might be useful in designing an ideal wound infection model for developing an effective wound healing formulation.

Download Full-text

Potent antibacterial activity of Dihydydropyrimidine-1,3,5-triazines via inhibition of DNA gyrase and antifungal activity with favourable metabolic profile

10.21203/rs.2.15956/v2 ◽

2019 ◽

Author(s):

Anup Masih ◽

Jitendra Kumar Shrivastava ◽

Hans Raj Bhat ◽

Udaya Pratap Singh

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Antifungal Activity ◽

Fungal Pathogens ◽

Dna Gyrase ◽

Infection Model ◽

Bacterial Strains ◽

Standard Drug

Abstract The compounds were tested against panel of three Gram-positive, viz. Staphylococcus aureus, Bacillus subtilis, Bacillus cereus and three Gram-negative bacterial strains viz. Pseudomonas aeruginosa, Escherichia coli, and Proteus vulgaris for determination of their antibacterial efficacy using cefixime as a standard drug. The antibiofilm activities of the compounds were determined against Staphylococcus aureus, and Bacillus subtilis. The most potent compounds 7l amd 7m found bacteriostatic in time kill assay via inhibition of DNA Gyrase enzyme. The metabolic liability of compound 7m was determined using RS-Predictor and MetaPrint 2D React. The antifungal activity against human fungal pathogens was also estimated, where these compounds showed considerable activity in comparison to standard. The in vivo antibacterial activity of compound 7m was also determined using S. aureus induced murine infection model.

Download Full-text

Potent antibacterial activity of Dihydydropyrimidine-1,3,5-triazines via inhibition of DNA gyrase and antifungal activity with favourable metabolic profile

10.21203/rs.2.15956/v1 ◽

2019 ◽

Author(s):

Anup Masih ◽

Jitendra Kumar Shrivastava ◽

Hans Raj Bhat ◽

Udaya Pratap Singh

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Antifungal Activity ◽

Fungal Pathogens ◽

Dna Gyrase ◽

Infection Model ◽

Bacterial Strains ◽

Standard Drug

Abstract The compounds were tested against panel of three Gram-positive, viz. Staphylococcus aureus, Bacillus subtilis, Bacillus cereus and three Gram-negative bacterial strains viz. Pseudomonas aeruginosa, Escherichia coli, and Proteus vulgaris for determination of their antibacterial efficacy using cefixime as a standard drug. The antibiofilm activities of the compounds were determined against Staphylococcus aureus, and Bacillus subtilis. The most potent compounds 7l amd 7m found bacteriostatic in time kill assay via inhibition of DNA Gyrase enzyme. The metabolic liability of compound 7m was determined using RS-Predictor and MetaPrint 2D React. The antifungal activity against human fungal pathogens was also estimated, where these compounds showed considerable activity in comparison to standard. The in vivo antibacterial activity of compound 7m was also determined using S. aureus induced murine infection model.

Download Full-text

Antimicrobial photodynamic therapy in chronic osteomyelitis induced by Staphylococcus aureus: An in vitro and in vivo study

10.1063/1.4757830 ◽

2012 ◽

Author(s):

João Alves dos Reis Júnior ◽

Patrícia Nascimento de Assis ◽

Garde^nia Matos Paraguassú ◽

Isabele Cardoso Vieira de de Castro ◽

Renan Ferreira Trindade ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Photodynamic Therapy ◽

Chronic Osteomyelitis ◽

In Vivo Study ◽

Antimicrobial Photodynamic Therapy

Download Full-text

Photo-Crosslinked Polymeric Matrix with Antimicrobial Functions for Excisional Wound Healing in Mice

Nanomaterials ◽

10.3390/nano8100791 ◽

2018 ◽

Vol 8 (10) ◽

pp. 791 ◽

Cited By ~ 3

Author(s):

Ming-Hsiang Chang ◽

Yu-Ping Hsiao ◽

Chia-Yen Hsu ◽

Ping-Shan Lai

Keyword(s):

Wound Healing ◽

Wound Infection ◽

Polymer Solution ◽

Wound Dressing ◽

Glycol Diacrylate ◽

Excisional Wound ◽

Poly Ethylene ◽

Systemic Infections

Wound infection extends the duration of wound healing and also causes systemic infections such as sepsis, and, in severe cases, may lead to death. Early prevention of wound infection and its appropriate treatment are important. A photoreactive modified gelatin (GE-BTHE) was synthesized by gelatin and a conjugate formed from the 3,3′,4,4′-benzophenone tetracarboxylic dianhydride (BTDA) and the 2-hydroxyethyl methacrylate (HEMA). Herein, we investigated the photocurable polymer solution (GE-BTHE mixture) containing GE-BTHE, poly(ethylene glycol) diacrylate (PEGDA), chitosan, and methylene blue (MB), with antimicrobial functions and photodynamic antimicrobial chemotherapy for wound dressing. This photocurable polymer solution was found to have fast film-forming property attributed to the photochemical reaction between GE-BTHE and PEGDA, as well as the antibacterial activity in vitro attributed to the ingredients of chitosan and MB. Our in vivo results also demonstrated that untreated wounds after 3 days had the same scab level as the GE-BTHE mixture-treated wounds after 20 s of irradiation, which indicates that the irradiated GE-BTHE mixture can be quickly transferred into artificial scabs to protect wounds from an infection that can serve as a convenient excisional wound dressing with antibacterial efficacy. Therefore, it has the potential to treat nonhealing wounds, deep burns, diabetic ulcers and a variety of mucosal wounds.

Download Full-text

Anti-inflammatory and wound healing properties of Rubus fairholmianus Gard. root—An in vivo study

Industrial Crops and Products ◽

10.1016/j.indcrop.2014.01.037 ◽

2014 ◽

Vol 54 ◽

pp. 216-225 ◽

Cited By ~ 20

Author(s):

Blassan P. George ◽

Thangaraj Parimelazhagan ◽

Rahul Chandran

Keyword(s):

Wound Healing ◽

In Vivo Study ◽

Anti Inflammatory

Download Full-text

Role of BrnQ1 and BrnQ2 in Branched-Chain Amino Acid Transport and Virulence in Staphylococcus aureus

Infection and Immunity ◽

10.1128/iai.02542-14 ◽

2014 ◽

Vol 83 (3) ◽

pp. 1019-1029 ◽

Cited By ~ 21

Author(s):

Julienne C. Kaiser ◽

Sameha Omer ◽

Jessica R. Sheldon ◽

Ian Welch ◽

David E. Heinrichs

Keyword(s):

Staphylococcus Aureus ◽

Virulence Gene ◽

Defined Medium ◽

Infection Model ◽

Content Type ◽

Virulence Gene Expression ◽

Branched Chain Amino Acid ◽

Branched Chain

The branched-chain amino acids (BCAAs; Ile, Leu, and Val) not only are important nutrients for the growth ofStaphylococcus aureusbut also are corepressors for CodY, which regulates virulence gene expression, implicating BCAAs as an important link between the metabolic state of the cell and virulence. BCAAs are either synthesized intracellularly or acquired from the environment.S. aureusencodes three putative BCAA transporters, designated BrnQ1, BrnQ2, and BrnQ3; their functions have not yet been formally tested. In this study, we mutated all threebrnQparalogs so as to characterize their substrate specificities and their roles in growthin vitroandin vivo. We demonstrated that in the community-associated, methicillin-resistantS. aureus(CA-MRSA) strain USA300, BrnQ1 is involved in uptake of all three BCAAs, BrnQ2 transports Ile, and BrnQ3 does not have a significant role in BCAA transport under the conditions tested. Of the three, only BrnQ1 is essential for USA300 to grow in a chemically defined medium that is limited for Leu or Val. Interestingly, we observed that abrnQ2mutant grew better than USA300 in media limited for Leu and Val, owing to the fact that this mutation leads to overexpression ofbrnQ1. In a murine infection model, thebrnQ1mutant was attenuated, but in contrast,brnQ2mutants had significantly increased virulence compared to that of USA300, a phenotype we suggest is at least partially linked to enhancedin vivoscavenging of Leu and Val through BrnQ1. These data uncover a hitherto-undiscovered connection between nutrient acquisition and virulence in CA-MRSA.

Download Full-text

In vitro and in vivo evaluation of antibacterial activity of Bridelia ferruginea extracts on some clinical isolates

The Journal of Phytopharmacology ◽

10.31254/phyto.2018.7407 ◽

2018 ◽

Vol 7 (4) ◽

pp. 392-398

Author(s):

B.T Yunana ◽

◽

B. B Bukar ◽

J. C Aguiyi ◽

◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bark Extract ◽

Root Extract ◽

Root Bark ◽

Zone Of Inhibition ◽

Bridelia Ferruginea

The ethanol extracts of root, bark and leaf of Bridelia ferruginea was investigated for antibacterial activity against clinical isolate of Staphylococcus aureus and Escherichia coli. The extracts had significant antibacterial activity in vitro at concentration of 25 mg/ml, 50 mg/ml, 100 mg/ml and 200 mg/ml and in vivo at dose of 50 mg/kg and 100 mg/kg. The root extract in vitro had the highest zone of inhibition, followed by the bark extract for both Staphylococcus aureus and Escherichia coli. The concentration of 200 mg/ml had the highest zone of inhibition in vitro. The minimum inhibitory concentration (MIC) showed a decreasing inhibitory effect of the plant extracts for both Staphylococcus aureus and Escherichia coli as the concentration decreases with root having 3.125 mg/ml, bark having 6.25 mg/ml and leaf having 25 mg/ml for Staphylococcus aureus and Escherichia coli. Likewise, the minimum bactericidal concentration (MBC) showed decreasing bactericide effects with decrease concentration with root having 12.5 mg/ml, bark having 12.5 mg/ml and leaf having 25 mg/ml for Escherichia coli while root had 6.25mg/ml, bark had 12.5mg/ml and leaf had 25mg/ml for Staphylococcus aureus. The in vivo investigation showed that the root and bark extract exhibited antibacterial activity on both Staphylococcus aureus and Escherichia coli at doses of 100mg/kg and 50mg/kg; the root extract had higher activity than the bark and root/bark combined. The dose of 100 mg/kg had the highest colonies reduction for Staphylococcus aureus and Escherichia coli in vivo. Preliminary phytochemical screening of root, bark and leaves of Bridelia ferruginea revealed the presence of tannins, flavonoids, carbohydrates, cardiac glycoside (root, bark and leaves), saponins (root and bark). The presence of tannins, saponins, flavonoid, cardiac glycoside and carbohydrate in the bark and root extracts of the plant indicates that the bark and root extracts were pharmacological importance

Download Full-text

IL-17C and IL-17RE Promote Wound Closure in a Staphylococcus aureus-Based Murine Wound Infection Model

Microorganisms ◽

10.3390/microorganisms9091821 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1821

Author(s):

Linda Pätzold ◽

Alexandra Stark ◽

Felix Ritzmann ◽

Carola Meier ◽

Thomas Tschernig ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Wound Infection ◽

Wound Closure ◽

Skin Diseases ◽

Infection Model ◽

Interleukin 17 ◽

Immune Mediated ◽

Significant Difference ◽

Infected Skin

The epithelial cytokine interleukin-17C (IL-17C) mediates inflammation through the interleukin 17 receptor E (IL-17RE). Prior studies showed a detrimental role of IL-17C in the pathogenesis of immune-mediated skin diseases (e.g., psoriasis). Here, we examined the role of IL-17C/IL-17RE in wound closure in a Staphylococcus aureus wound infection model. We demonstrate that wound closure is significantly delayed in IL-17RE (Il-17re−/−)- and 17C (Il-17c−/−)-deficient mice. There was no significant difference between WT, Il-17re−/−, and Il-17c−/− mice in the absence of infection. Deficiency for IL-17RE and IL-17C did not significantly affect the elimination of bacteria. IL-17C expression was increased in the epidermis of human S. aureus-infected skin. Our results indicate that the IL-17C/IL-17RE axis contributes to the closure of infected wounds but does not contribute to the elimination of S. aureus.

Download Full-text