scholarly journals Pancreatic duct ligation reduces premalignant pancreatic lesions in a Kras model of pancreatic adenocarcinoma in mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Marta Cáceres ◽  
Rita Quesada ◽  
Mar Iglesias ◽  
Francisco X. Real ◽  
Maria Villamonte ◽  
...  
Keyword(s):  
Pancreatic Duct ◽  
Premalignant Lesions ◽  
Ductal Adenocarcinoma ◽  
High Grade ◽  
Histological Changes ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation ◽  
Risk Patients ◽  
Flat Lesions ◽  
Over Time

Abstract Pancreatic duct ligation (PDL) in the murine model has been described as an exocrine pancreatic atrophy-inducing procedure. However, its influence has scarcely been described on premalignant lesions. This study describes the histological changes of premalignant lesions and the gene expression in a well-defined model of pancreatic ductal adenocarcinoma by PDL. Selective ligation of the splenic lobe of the pancreas was performed in Ptf1a-Cre(+/ki); K-ras LSLG12Vgeo(+/ki) mice (PDL-Kras mice). Three experimental groups were evaluated: PDL group, controls and shams. The presence and number of premalignant lesions (PanIN 1–3 and Atypical Flat Lesions—AFL) in proximal (PP) and distal (DP) pancreas were studied for each group over time. Microarray analysis was performed to find differentially expressed genes (DEG) between PP and PD. Clinical human specimens after pancreaticoduodenectomy with ductal occlusion were also evaluated. PDL-Kras mice showed an intense pattern of atrophy in DP which was shrunk to a minimal portion of tissue. Mice in control and sham groups had a 7 and 10-time increase respectively of risk of high-grade PanIN 2 and 3 and AFL in their DP than PDL-Kras mice. Furthermore, PDL-Kras mice had significantly less PanIN 1 and 2 and AFL lesions in DP compared to PP. We identified 38 DEGs comparing PP and PD. Among them, several mapped to protein secretion and digestion while others such as Nupr1 have been previously associated with PanIN and PDAC. PDL in Ptf1a-Cre(+/ki); K-ras LSLG12Vgeo(+/ki) mice induces a decrease in the presence of premalignant lesions in the ligated DP. This could be a potential line of research of interest in some cancerous risk patients.

scholarly journals Publisher Correction: Pancreatic duct ligation reduces premalignant pancreatic lesions in a Kras model of pancreatic adenocarcinoma in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marta Cáceres ◽  
Rita Quesada ◽  
Mar Iglesias ◽  
Francisco X. Real ◽  
Maria Villamonte ◽  
...  
Keyword(s):  
Pancreatic Duct ◽  
Pancreatic Adenocarcinoma ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Pre-Operative Imaging and Pathological Diagnosis of Localized High-Grade Pancreatic Intra-Epithelial Neoplasia without Invasive Carcinoma

Cancers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 945
Author(s):  
Ryota Sagami ◽  
Kentaro Yamao ◽  
Jun Nakahodo ◽  
Ryuki Minami ◽  
Masakatsu Tsurusaki ◽  
...  
Keyword(s):  
Pancreatic Duct ◽  
Invasive Carcinoma ◽  
Current Knowledge ◽  
Precancerous Lesions ◽  
Ductal Adenocarcinoma ◽  
Pancreatic Ducts ◽  
High Grade ◽  
Cytologic Examination ◽  
Imaging Characteristics ◽  
Retention Cysts

Pancreatic ductal adenocarcinoma (PDAC) arises from precursor lesions, such as pancreatic intra-epithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasm (IPMN). The prognosis of high-grade precancerous lesions, including high-grade PanIN and high-grade IPMN, without invasive carcinoma is good, despite the overall poor prognosis of PDAC. High-grade PanIN, as a lesion preceding invasive PDAC, is therefore a primary target for intervention. However, detection of localized high-grade PanIN is difficult when using standard radiological approaches. Therefore, most studies of high-grade PanIN have been conducted using specimens that harbor invasive PDAC. Recently, imaging characteristics of high-grade PanIN have been revealed. Obstruction of the pancreatic duct due to high-grade PanIN may induce a loss of acinar cells replaced by fibrosis and lobular parenchymal atrophy. These changes and additional inflammation around the branch pancreatic ducts (BPDs) result in main pancreatic duct (MPD) stenosis, dilation, retention cysts (BPD dilation), focal pancreatic parenchymal atrophy, and/or hypoechoic changes around the MPD. These indirect imaging findings have become important clues for localized, high-grade PanIN detection. To obtain pre-operative histopathological confirmation of suspected cases, serial pancreatic-juice aspiration cytologic examination is effective. In this review, we outline current knowledge on imaging characteristics of high-grade PanIN.

scholarly journals Somatostatin levels and binding in duodenal mucosa of rabbits with ligation of the pancreatic duct

1988 ◽  
Vol 118 (2) ◽  
pp. 227-232 ◽  
Author(s):  
L. G. Guijarro ◽  
E. Arilla
Keyword(s):  
Pancreatic Duct ◽  
Binding Sites ◽  
Exocrine Pancreas ◽  
Duodenal Mucosa ◽  
Physiological Significance ◽  
Scatchard Analysis ◽  
Pancreatic Duct Ligation ◽  
Parallel Increase ◽  
Duct Ligation ◽  
Number Of Binding Sites

ABSTRACT Atrophy of the exocrine pancreas was induced in rabbits by pancreatic duct ligation. Somatostatin concentration and binding in cytosol from rabbit duodenal mucosa were studied after 6 and 14 weeks of pancreatic duct ligation. Somatostatin-like immunoreactivity was significantly increased in the duodenal mucosa in both periods. Scatchard analysis showed a parallel increase in the number of binding sites rather than a change in their affinity. The physiological significance of these findings remains to be clarified. J. Endocr. (1988) 118, 227–232

Apoptosis associated gene expressions in pancreas after pancreatic duct ligation in rats

2001 ◽  
Vol 120 (5) ◽  
pp. A720
Author(s):  
Hong Sik Lee ◽  
Chang Duck Kim ◽  
Byung Won Hur ◽  
Chang Don Kang ◽  
Hoon Jai Chun ◽  
...  
Keyword(s):  
Pancreatic Duct ◽  
Gene Expressions ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation

Influence of pancreatic duct ligation on endocrine and exocrine rat pancreas

1983 ◽  
Vol 117 (2) ◽  
pp. 281-286 ◽  
Author(s):  
GUNNAR ISAKSSON ◽  
INGEMAR IHSE ◽  
INGMAR LUNDQUIST
Keyword(s):  
Pancreatic Duct ◽  
Rat Pancreas ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation

Apoptosis is the dominant form of acinar cell death after pancreatic duct ligation in rats

1995 ◽  
Vol 108 (4) ◽  
pp. A399
Author(s):  
M. Wada ◽  
R. Doi ◽  
R. Hosotani ◽  
J. Lee ◽  
M. Imamura
Keyword(s):  
Cell Death ◽  
Pancreatic Duct ◽  
Acinar Cell ◽  
Dominant Form ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation
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