Serum trace metal association with response to erythropoiesis stimulating agents in incident and prevalent hemodialysis patients

AbstractAlterations in hemodialysis patients’ serum trace metals have been documented. Early studies addressing associations levels of serum trace metals with erythropoietic responses and/or hematocrit generated mixed results. These studies were conducted prior to current approaches for erythropoiesis stimulating agent (ESA) drug dosing guidelines or without consideration of inflammation markers (e.g. hepcidin) important for regulation of iron availability. This study sought to determine if the serum trace metal concentrations of incident or chronic hemodialysis patients associated with the observed ESA response variability and with consideration to ESA dose response, hepcidin, and high sensitivity C-reactive protein levels. Inductively-coupled plasma-mass spectrometry was used to measure 14 serum trace metals in 29 incident and 79 prevalent dialysis patients recruited prospectively. We compared these data to three measures of ESA dose response, sex, and dialysis incidence versus dialysis prevalence. Hemoglobin was negatively associated with ESA dose and cadmium while positively associated with antimony, arsenic and lead. ESA dose was negatively associated with achieved hemoglobin and vanadium while positively associated with arsenic. ESA response was positively associated with arsenic. Vanadium, nickel, cadmium, and tin were increased in prevalent patients. Manganese was increased in incident patients. Vanadium, nickel, and arsenic increased with time on dialysis while manganese decreased. Changes in vanadium and manganese were largest and appeared to have some effect on anemia. Incident and prevalent patients’ chromium and antimony levels exceeded established accepted upper limits of normal.

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Extended erythrocyte and reticulocyte parameters in the diagnosis of iron deficiency in hemodialysis patients

Chinese Journal of Medical Research ◽

10.37515/cjmr.091x.3405 ◽

2020 ◽

Vol 3 (4) ◽

pp. 109-113

Author(s):

Mohammed Nazim Bennaoum ◽

◽

Affaf Adda ◽

Mohamed Chekkal ◽

Fatima Seghier ◽

...

Keyword(s):

Iron Deficiency ◽

Iron Status ◽

High Sensitivity ◽

Hemodialysis Patients ◽

Chronic Hemodialysis ◽

Red Cells ◽

Corpuscular Hemoglobin ◽

Cellular Concentration ◽

Stage Renal Disease ◽

End Stage

Objective: Iron deficiency (ID) is a frequent complication in end stage renal insufficiency. These patients have to be diagnosed and treated to reduce the prevalence of anemia. Functional iron deficiency (FID) is a situation that can disrupt biochemical iron tests and mask an eventual association with ID. In this study, we tried to prove the ability of extended parameters of red cells and reticulocytes to diagnose ID without being influenced by FID. Design and methods: 164 chronic hemodialysis patients (CHP) in end stage renal disease were enrolled. Research parameters of red cells and reticulocytes determined on ADVIA 2120i were studied in the diagnosis of ID associated or not with chronic inflammation. Results: Parameters such as corpuscular hemoglobin of mature red cells (CHm), corpuscular hemoglobin of reticulocytes (CHr), cellular concentration of hemoglobin in mature red cells (CHCMm), cellular concentration of hemoglobin in reticulocytes (CHCMr) and percentage of microcytic and hypochromic red cells (HYMI) showed a high sensitivity to diagnose ID. However, the distinction of combined iron deficiency (CID) from other entities was not possible with all parameters. In chronic inflammatory states, the decrease of CHm, CHCMm and CHCMr with the rise of percentage hypochromic mature red cells (HYPOm) and reticulocytes (HYPOr) is in favor of CID. So, determination of inflammatory state is needed to complete research parameters of blood count in CHP. Conclusion: Extended erythrocyte and reticulocyte parameters can be useful to check iron status in CHP.

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Mobility, binding behavior and potential risks of trace metals in the sediments of the fifth largest freshwater lake, China

Water Science & Technology ◽

10.2166/wst.2013.099 ◽

2013 ◽

Vol 67 (11) ◽

pp. 2503-2510 ◽

Cited By ~ 6

Author(s):

Guolian Li ◽

Guijian Liu ◽

Chuncai Zhou ◽

Yu Kang ◽

Wanqing Yuan ◽

...

Keyword(s):

Trace Metals ◽

Trace Metal ◽

Inductively Coupled Plasma ◽

Optical Emission ◽

Spatial Diversity ◽

Emission Spectrometry ◽

Chaohu Lake ◽

Eutrophic Lakes ◽

Binding Behavior ◽

Potential Risks

The trace metal pollution of sediments in Chaohu Lake, one of the most highly eutrophic lakes in East China, was investigated. Surface sediment (0–5 cm) samples were collected from 35 different positions and analyzed by inductively coupled plasma optical emission spectrometry to determine trace metal contents. Results showed that the mean content of trace metals was as follows: Cr, 85.09 mg kg−1; Cu, 34.49 mg kg−1; Ni, 26.46 mg kg−1; Pb, 34.17 mg kg−1 and Zn, 107.46 mg kg−1. The trace metal concentrations from different sampling sites displayed spatial diversity; the heavily polluted sampling sites were close to where estuaries flow in to the lake. A four-step sequential extraction was used to examine the partitioning of the trace metals. Results demonstrated that the percentage of the species bound to the oxidizable phase for all trace metals ranged from 15.6 to 37.7%, while for Cu, Cr and Ni, the main forms were residual (41.3, 62.3 and 69.8%, respectively). Trace metals in the oxidizable fraction may mainly exist in the form of sulfides. The ecological potential risks of trace metals decreased as follows: Pb > Zn > Cu > Cr > Ni.

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Preparation of Urine Samples for Trace Metal Determination: A Study with Aluminium Analysis by Inductively Coupled Plasma Optical Emission Spectrometry

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456329803500208 ◽

1998 ◽

Vol 35 (2) ◽

pp. 245-253 ◽

Cited By ~ 10

Author(s):

T J Burden ◽

M W Whitehead ◽

R P H Thompson ◽

J J Powell

Keyword(s):

Trace Metals ◽

Trace Metal ◽

Inductively Coupled Plasma ◽

Optical Emission ◽

Urine Samples ◽

Emission Spectrometry ◽

Individual Sample ◽

Inductively Coupled ◽

Plasma Optical Emission Spectrometry ◽

Pooled Sample

Urinary analysis of trace metals forms a significant role in clinical chemistry, but the optimal preparation and analysis of urine samples has not been investigated. Human urine is generally supersaturated with dissolved solids. Therefore, samples often precipitate following collection. X-ray microanalysis showed that this precipitate was predominantly rich in calcium and phosphorus but could include some trace metals from urine, potentially lowering their concentrations in solution. Hence, the precipitate must be fully redissolved for accurate analysis of trace metals in urine. Methods are emphasized for the best collection and preparation of urine samples for subsequent trace metal analysis; in this work inductively coupled plasma optical emission spectrometry (ICPOES) was used for the analysis of aluminium. For optimal accuracy, peak profiles were collected over 396.147 nm-396.157 nm. Urinary aluminium levels were investigated from 10 healthy volunteers and concentrations were obtained using either aqueous, pooled or individual urine-based standard curves. Since urine has a highly variable matrix, individual sample-based standards, which are unique to that particular sample, gave the most accurate results. However, where sample size is small or sample numbers are unfeasibly large, pooled sample-based standards give good approximations to within 15% and, with appropriate validation, other elements as internal standards could also be used for approximations. Aqueous standards should be avoided. Spike-recovery experiments confirmed these data since individual sample based standards showed optimal recovery [99.3 (4.4)%], while pooled sample-based standards were a close proxy [101.6 (9.2)%] but aqueous standards were inappropriate [137.4 (12.8)%]. Postprandial urinary aluminium levels of the 10 volunteers were [7.2 (3.7)μg/L] after analysis using individual sample-based standard curves.

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Trace metal extractability in soils and uptake by bromegrass 20 years after sewage sludge application

Canadian Journal of Soil Science ◽

10.4141/s01-023 ◽

2002 ◽

Vol 82 (3) ◽

pp. 323-333 ◽

Cited By ~ 16

Author(s):

M B McBride ◽

L J Evans

Keyword(s):

Trace Metals ◽

Sewage Sludge ◽

Trace Metal ◽

Inductively Coupled Plasma ◽

Organic Matter Content ◽

Calcareous Soils ◽

Matter Content ◽

Plant Availability ◽

Sludge Application

The long-term potential for sewage sludge amendments to alter forage trace metal concentrations was determined on bromegrass, which received no sludge application for 20 yr following annual application during 1973-1980 on a no-till randomized-plot experimental site. In addition, soils were analyzed for trace metals, both total and extractable (using hot 0.01 M CaCl2), by axial-view inductively coupled plasma spectrometry. For Cu, Ni, Zn and Cd, extractability from the soil (by CaCl2) as well as plant tissue concentrations were relatively low considering the high loadings of these particular metals, a fact attributable to the near-neutral pH and high organic matter content of these calcareous soils. However, total and extractable soil Mo remained elevated in the sewage-sludge-amended plots above the levels measured in the fertilized control plots some 20 yr after the last sewage sludge application. Mo bioaccumulated in the bromegrass more than the other trace metals investigated at this site. The Cu:Mo ratio in the forage, a measure of potential to induce Cu deficiency in grazing ruminants, was substantially depressed by most of the sewage sludge treatments. Comparison of the 2000 and 1980 bromegrass analyses suggests an increase in the long-term plant availability of Mo in soils amended with high-Fe and high-Al sludges. Conversely, there was a long-term decrease in Mo availability on the high-Ca sludge treatment, consistent with evidence that much of the Mo has been lost from the topsoil since sludge application. For Cu, Ni and Zn, plant availability appears to have decreased between 1980 and 2000, attributable in part to a calculated loss of substantial fractions of these metals from the topsoil. Bromegrass concentrations of Cu, Ni, Zn, Cd and Mo were strongly correlated to CaCl2-extractable and total metals in the soils, indicating the utility of 0.01 M CaCl2 in predicting plant availability. The chemical differences in the three sludge types applied at the site (high-Ca, high-Al, high-Fe) had little long-term impact on the extractability and phytoavailability of most trace metals remaining in the topsoil. Key words: Biosolids, molybdenum, copper, forage, cadmium, heavy metals

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Moderate elevations of high-sensitivity cardiac troponin I and B-type natriuretic peptide in chronic hemodialysis patients are associated with mortality

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2012-0305 ◽

2013 ◽

Vol 51 (6) ◽

Cited By ~ 2

Author(s):

Daniël A. Geerse ◽

Miranda van Berkel ◽

Steffie Vogels ◽

Jeroen P. Kooman ◽

Constantijn J.A.M. Konings ◽

...

Keyword(s):

Natriuretic Peptide ◽

Cardiac Troponin ◽

Troponin I ◽

Troponin T ◽

Total Mortality ◽

High Sensitivity ◽

Hemodialysis Patients ◽

Chronic Hemodialysis ◽

Cardiac Troponin I ◽

Moderate Elevation

AbstractSeveral biomarkers are associated with mortality in hemodialysis patients. In particular, elevated cardiac troponin T and B-type natriuretic peptide (BNP) are strong predictors of mortality; however, less is known about cardiac troponin I (cTnI). Elevated troponin I is detected in many hemodialysis patients, but the association of moderate elevations with mortality is unclear.The relation between mortality and cTnI, using a high-sensitivity cTnI assay, as well as BNP and C-reactive protein (CRP) was evaluated in 206 chronic hemodialysis patients.Median follow-up was 28 months with a total mortality of 35%. Mortality was significantly associated with elevated cTnI, BNP and CRP. Even patients with only moderate elevation of cTnI (0.01–0.10 μg/L) showed 2.5-fold increased mortality. Interestingly, hazard ratios for mortality for single (random) measurements were comparable to those for mean/median measurements. Subsequently, subgroup analysis based on combined markers was performed. Patients with both cTnI <0.01 μg/L and BNP in the first quartile had 100% survival. Patients with either cTnI <0.01 μg/L or BNP in the lowest quartile had significantly lower mortality (12% and 13%, respectively) than patients with BNP levels in the second quartile or higher and cTnI of 0.01–0.05 μg/L and patients with cTnI ≥0.05 μg/L (mortality 46 and 58%, respectively).A combination of moderate elevation of cTnI and BNP provided additional prognostic value. A single measurement of these biomarkers performed comparably to the mean/median of multiple measurements.

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Flaxseed oil supplementation decreases C-reactive protein levels in chronic hemodialysis patients

Nutrition Research ◽

10.1016/j.nutres.2012.08.007 ◽

2012 ◽

Vol 32 (12) ◽

pp. 921-927 ◽

Cited By ~ 31

Author(s):

Joana R.N. Lemos ◽

Mariana Gascue de Alencastro ◽

Anita Vieceli Konrath ◽

Marina Cargnin ◽

Roberto Ceratti Manfro

Keyword(s):

Hemodialysis Patients ◽

Flaxseed Oil ◽

Chronic Hemodialysis ◽

C Reactive Protein ◽

Protein Levels ◽

Reactive Protein

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The association of QT dispersion and high-sensitivity C-reactive protein levels in hemodialysis patients

Journal of Electrocardiology ◽

10.1016/j.jelectrocard.2007.03.392 ◽

2007 ◽

Vol 40 (4) ◽

pp. S59-S60

Author(s):

Ahmet Oktay ◽

Ali Serdar Fak ◽

A. Altug Cincin ◽

Ahmet Toprak ◽

Mehmet Koc ◽

...

Keyword(s):

High Sensitivity ◽

Qt Dispersion ◽

Hemodialysis Patients ◽

C Reactive Protein ◽

Protein Levels ◽

Reactive Protein

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Composition of Trace Metals in Dust Samples Collected from Selected High Schools in Pretoria, South Africa

Applied and Environmental Soil Science ◽

10.1155/2016/5829657 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 2

Author(s):

J. O. Olowoyo ◽

L. L. Mugivhisa ◽

Z. G. Magoloi

Keyword(s):

High Schools ◽

Trace Metals ◽

Trace Metal ◽

Inductively Coupled Plasma ◽

Common Source ◽

Residential Areas ◽

Potential Health ◽

Inductively Coupled ◽

Positive Effects ◽

Recess Period

Potential health risks associated with trace metal pollution have necessitated the importance of monitoring their levels in the environment. The present study investigated the concentrations and compositions of trace metals in dust samples collected from classrooms and playing ground from the selected high schools In Pretoria. Schools were selected from Pretoria based on factors such as proximity to high traffic ways, industrial areas, and residential areas. Thirty-two dust samples were collected from inside and outside the classrooms, where learners often stay during recess period. The dust samples were analysed for trace metal concentrations using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). The composition of the elements showed that the concentrations of Zn were more than all other elements except from one of the schools. There were significant differences in the concentrations of trace metals from the schools (p<0.05). Regular cleaning, proximity to busy road, and well maintained gardens seem to have positive effects on the concentrations of trace metals recorded from the classrooms dust. The result further revealed a positive correlation for elements such as Pb, Cu, Zn, Mn, and Sb, indicating that the dust might have a common source.

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Coprecipitation of Co2+, Ni2+ and Zn2+ with Mn(III/IV) Oxides Formed in Metal-Rich Mine Waters

Minerals ◽

10.3390/min9040226 ◽

2019 ◽

Vol 9 (4) ◽

pp. 226 ◽

Cited By ~ 6

Author(s):

Sánchez-España ◽

Yusta

Keyword(s):

Trace Metals ◽

Trace Metal ◽

Mine Water ◽

Inductively Coupled Plasma ◽

Manganese Oxides ◽

Mine Waters ◽

X Ray ◽

Inductively Coupled ◽

Mn Oxides ◽

Very High

Manganese oxides are widespread in soils and natural waters, and their capacity to adsorb different trace metals such as Co, Ni, or Zn is well known. In this study, we aimed to compare the extent of trace metal coprecipitation in different Mn oxides formed during Mn(II) oxidation in highly concentrated, metal-rich mine waters. For this purpose, mine water samples collected from the deepest part of several acidic pit lakes in Spain (pH 2.7–4.2), with very high concentration of manganese (358–892 mg/L Mn) and trace metals (e.g., 795–10,394 µg/L Ni, 678–11,081 µg/L Co, 259–624 mg/L Zn), were neutralized to pH 8.0 in the laboratory and later used for Mn(II) oxidation experiments. These waters were subsequently allowed to oxidize at room temperature and pH = 8.5–9.0 over several weeks until Mn(II) was totally oxidized and a dense layer of manganese precipitates had been formed. These solids were characterized by different techniques for investigating the mineral phases formed and the amount of coprecipitated trace metals. All Mn oxides were fine-grained and poorly crystalline. Evidence from X-Ray Diffraction (XRD) and Scanning Electron Microscopy coupled to Energy Dispersive X-Ray Spectroscopy (SEM–EDX) suggests the formation of different manganese oxides with varying oxidation state ranging from Mn(III) (e.g., manganite) and Mn(III/IV) (e.g., birnessite, todorokite) to Mn(IV) (e.g., asbolane). Whole-precipitate analyses by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS), Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES), and/or Atomic Absorption Spectrometry (AAS), provided important concentrations of trace metals in birnessite (e.g., up to 1424 ppm Co, 814 ppm Ni, and 2713 ppm Zn), while Co and Ni concentrations at weight percent units were detected in asbolane by SEM-EDX. This trace metal retention capacity is lower than that observed in natural Mn oxides (e.g., birnessite) formed in the water column in a circum-neutral pit lake (pH 7.0–8.0), or in desautelsite obtained in previous neutralization experiments (pH 9.0–10.0). However, given the very high amount of Mn sorbent material formed in the solutions (2.8–4.6 g/L Mn oxide), the formation of these Mn(III/IV) oxides invariably led to the virtually total removal of Co, Ni, and Zn from the aqueous phase. We evaluate these data in the context of mine water pollution treatment and recovery of critical metals.

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