scholarly journals Identification and quantification of target metabolites combined with transcriptome of two rheum species focused on anthraquinone and flavonoids biosynthesis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jing Liu ◽  
Liang Leng ◽  
Yan Liu ◽  
Han Gao ◽  
Wei Yang ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Biosynthetic Pathway ◽  
Multiple Reaction Monitoring ◽  
Perennial Herb ◽  
Reaction Monitoring ◽  
Transcriptome Data ◽  
Quadrupole Mass ◽  
Peak Intensity ◽  
Flavonoids Biosynthesis ◽  
Important Medicinal Plant

AbstractRheum emodi is a perennial herb and an important medicinal plant, with anthraquinones and flavonoids as its main bioactive compounds. However, there is little knowledge about the biosynthetic pathway of anthraquinones in rhubarbs. In this study, we qualitatively and quantitatively assessed 62 pharmacological metabolites in rhubarb using dynamic multiple reaction monitoring (dMRM) of triple-quadrupole mass spectrometry (QqQ-MS), including 21 anthraquinones, 17 flavonoids, 6 stilbenes, 12 gallate esters, 3 tannins, and 3 others. Besides, the metabolomics results showed significant differences among all the 60 metabolites, except for gallic acid and piceatannol-O-β-glucoside. The combined transcriptome data of R. palmatum L. (RPL) and R. officinale Baill. (ROB) showed that 21,691 unigenes were annotated in the metabolic pathways. Taken together, 17 differentially expressed genes (DEGs) were associated with the anthraquinone biosynthetic pathway. Additionally, a significant correlation between anthraquinone peak intensity and DEG expression level existed, validating that DEGs contribute to the anthraquinone biosynthetic pathway. RT-qPCR results showed that the cluster-14354.38156 gene may catalyze the O-methylation of emodin to produce physcion. This study provides a useful resource for further studies on secondary metabolism in rhubarb and the combination analysis of transcriptome and metabolome, which can help with the discovery of enzyme genes involved in metabolite biosynthesis.

scholarly journals Identification and Quantification of Flavanol Glycosides in Vitis vinifera Grape Seeds and Skins during Ripening

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2745 ◽  
Author(s):  
Marie Zerbib ◽  
Guillaume Cazals ◽  
Christine Enjalbal ◽  
Cédric Saucier
Keyword(s):  
Vitis Vinifera ◽  
High Performance ◽  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Quadrupole Mass ◽  
Grape Seeds ◽  
Grape Berries ◽  
Grape Skins ◽  
Grape Varieties ◽  
First Time

Monomeric and dimeric flavanol glycosides were analyzed in Vitis vinifera grapes and seeds during ripening. An analytical method using ultra-high performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UHPLC-ESI-QQQ-MS/MS) in multiple reaction monitoring (MRM) mode was employed. Three grape varieties (Merlot, Syrah and Tannat) were chosen and grape berries were sampled at different stages of development. Ten monoglycosylated and six diglycosylated flavanol monomers were detected. Twelve monoglycosylated and three diglycosylated flavanol dimers were also detected for all three grape varieties. All diglycosides were detected for the first time in Vitis vinifera grapes, though some of these compounds were only detected in skins or seeds. Furthermore, the evolution of all these compounds was studied, and a decrease in monomeric (epi) catechin monoglycosides was observed during ripening for Tannat, Merlot and Syrah grape skins. The dimers would appear to accumulate in skin tissues up to mid-summer (after veraison) and decrease when grape berries reached maturity.

scholarly journals Determination of Unsulfonated Aromatic Amines in FD&C Yellow No. 5 and FD&C Yellow No. 6 by Liquid Chromatography–Triple Quadrupole Mass Spectrometry

2019 ◽  
Vol 102 (2) ◽  
pp. 580-589
Author(s):  
Nebebech Belai ◽  
Samuel R White
Keyword(s):  
Aromatic Amines ◽  
Matrix Effects ◽  
Multiple Reaction Monitoring ◽  
Current Method ◽  
Regression Coefficients ◽  
Reaction Monitoring ◽  
Triple Quadrupole ◽  
Quadrupole Mass ◽  
The U.S

Abstract Background: This paper describes a simple and sensitive ultra-HPLC–triple quadrupole MS (LC-MS/MS) method for the determination of six unsulfonated aromatic amines in the color additives FD&C Yellow No. 5 (Y5) and FD&C Yellow No. 6 (Y6). The six amines determined by thismethod are aniline (ANL), benzidine (BNZ), 4-aminobiphenyl (4ABP), 4-aminoazobenzene (4AAB), 2-aminobiphenyl (2ABP), and 4-aminobenzonitrile (4ABN). Objective: This method is intended foruse in batch certification of the color additives by the U.S. Food and Drug Administration (FDA) to ensure that each lot meets published specifications for coloring foods, drugs, and cosmetics. Methods: A modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) procedure is used for extraction of the amines. Quantitative determination was performed in electrospray positive ionization and multiple-reaction monitoring modes. Results: Validation of the method demonstrated overall recovery of 101–115% and precision of 1.74–9.78% for all analytes. Excellent regression coefficients were obtained, with values >0.999. Conclusions: The validated method was successfully used for the analyses of 30 Y5 and Y6 samples and provided results that are consistent with results from the current method used by FDA, with greater sensitivity and low matrix effects. Highlights: The validation results demonstrate that the new LC-MS/MS method is applicable for use in routine batch certification.

scholarly journals Pulsed Multiple Reaction Monitoring Approach to Enhancing Sensitivity of a Tandem Quadrupole Mass Spectrometer

2011 ◽  
Vol 83 (6) ◽  
pp. 2162-2171 ◽  
Author(s):  
Mikhail E. Belov ◽  
Satendra Prasad ◽  
David C. Prior ◽  
William F. Danielson ◽  
Karl Weitz ◽  
...  
Keyword(s):  
Mass Spectrometer ◽  
Multiple Reaction Monitoring ◽  
Quadrupole Mass Spectrometer ◽  
Reaction Monitoring ◽  
Quadrupole Mass ◽  
Tandem Quadrupole Mass Spectrometer ◽  
Tandem Quadrupole
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