Chemical composition of Polish propolis and its antiproliferative effect in combination with Bacopa monnieri on glioblastoma cell lines

AbstractPropolis and Bacopa monnieri (L.) Wettst. (Brahmi) are natural products that contain many active substances and possess anticancer properties. The aim of this study was to investigate the chemical composition of Polish propolis extract (PPE) by gas chromatography-mass spectrometry (GC–MS), B. monnieri extracts (BcH, BcS) by high performance liquid chromatography with diode array detector and mass spectrometry coupled with electrospray ionization (LC–ESI–MS) and finally determine its anti-proliferative potential combined with BcH and BcS in glioblastoma cell lines (T98G, LN-18, U87MG). The antiproliferative activity of PPE, BcH, BcS and their combination (PPE + BcH) was determined by a cytotoxicity test, and DNA binding was determined by [3H]-thymidine incorporation. Flavonoids and phenylpropenoids were the main components of PPE. BcH and BcS samples were also successfully analyzed. Their main constituents were saponins such as bacoside A3, bacopaside II, X and bacopasaponin C and its isomer. The inhibitory effects on the viability and proliferation of the tested glioma cells observed after incubation with the combination of PPE and BcH were significantly stronger than the effects of these two extracts separately. These findings suggest that propolis in combination with B. monnieri shows promising anticancer activity for the treatment of glioblastoma. However, further studies are still required.

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Propolis from Poland versus propolis from New Zealand - chemical composition and antiproliferative properties on glioblastoma cell lines.

10.21203/rs.2.23267/v1 ◽

2020 ◽

Author(s):

Justyna Moskwa ◽

Sylwia Katarzyna Naliwajko ◽

Renata Markiewicz-Żukowska ◽

Krystyna Joanna Gromkowska-Kępka ◽

Katarzyna Socha ◽

...

Keyword(s):

New Zealand ◽

Chemical Composition ◽

Glioblastoma Multiforme ◽

Cell Lines ◽

Antiproliferative Activity ◽

Gas Chromatography Mass Spectrometry ◽

Cytotoxicity Test ◽

Diffuse Astrocytoma ◽

Grade Ii ◽

Grade Ii Glioma

Abstract Background Several studies have previously reported that propolis and its ingredients inhibit glioma cancer cell lines. The chemical composition and antiproliferative activity of propolis from Poland (PPE) and propolis from New Zealand (MPE) were compared in this study. Methods The chemical composition was investigated by gas chromatography-mass spectrometry. Antiproliferative activity of PPE and MPE was determined by a cytotoxicity test and DNA binding by [ 3 H]-thymidine incorporation on Human Diffuse Astrocytoma cell line (DASC) derived from a patient with a Grade II glioma and glioblastoma multiforme T98G and LN-18 cell lines from American Type Culture Collection. Results The chemical composition of both propolis was comparable, with marginal differences in the amount of some compounds. Flavonoids and chalcones, of which pinocembrin, pinobanksin, pinobanksin 3-acetate, chrysin and galangin showed the highest level, were the main components of both examined propolis (PPE–49.4% and MPE–52.1%). The performed cytotoxicity test showed powerful activity of PPE and MPE propolis on DASC, T98G and LN-18 cells. The degree of the antiproliferative activity was similar in the case of both propolis (viability after 72 h for 30 µg/mL ranged from 22.0% to 51.6% and proliferation inhibition after 72 h approximately was from 18.6% to 75.6%). Conclusions These results are the first to show that propolis from Poland and propolis from New Zealand have a strong cytotoxic and antiproliferative effect on DASC (Grade II glioma) derived from a patient and glioblastoma multiforme T98G and LN-18 cell lines. This activity may be associated with the high content of polyphenolic compounds in both propolis. These findings suggest that Polish and New Zealand propolis shows promising anticancer activity in the treatment of glioblastoma. However, further studies are required.

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Development of barcode and proteome profiling of glioblastoma

Biomeditsinskaya Khimiya ◽

10.18097/pbmc20146003308 ◽

2014 ◽

Vol 60 (3) ◽

pp. 308-321 ◽

Cited By ~ 4

Author(s):

S.N. Naryzhny ◽

N.L. Ronzhina ◽

M.A. Mainskova ◽

N.V. Belyakova ◽

R.A. Pantina ◽

...

Keyword(s):

Mass Spectrometry ◽

Brain Tumor ◽

Cell Lines ◽

High Grade Glioma ◽

Initial Step ◽

High Grade ◽

Glioblastoma Cell ◽

Cancer Death ◽

Annexin 1 ◽

Glioblastoma Cell Lines

High grade glioma (glioblastoma) is the most common brain tumor. Its malignancy makes it the fourth biggest cause of cancer death. In our experiments we used several glioblastoma cell lines generated in our laboratory to obtain proteomics information specific for this disease. This study starts our developing the complete 2DE map of glioblastoma proteins. 2DE separation with following imaging, immunochemistry, spot picking, and mass-spectrometry allowed us detecting and identifying more than 100 proteins. Several of them have prominent differences in their level between norm and cancer. Among them are alpha-enolase (ENOA_HUMAN), pyruvate kinase isozymes M1/M2 (KPYM_HUMAN), cofilin 1 (COF1_HUMAN), translationally-controlled tumor protein TCTP_HUMAN, annexin 1 (ANXA1_HUMAN), PCNA (PCNA_HUMAN), p53 (TP53_HUMAN) and others. Most interesting results were obtained with protein p53. In all glioblastoma cell lines, its level was dramatically up regulated and enriched by multiple additional isoforms. This distribution is well correlated with presence of these proteins inside of cells themselves. At this initial step we suggest the panel of specific brain tumor markers (signature) to help creating noninvasive techniques to diagnose disease. These preliminary data point to these proteins as promising markers of glioblastoma.

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