scholarly journals Propolis from Poland versus propolis from New Zealand - chemical composition and antiproliferative properties on glioblastoma cell lines.

2020 ◽  
Author(s):  
Justyna Moskwa ◽  
Sylwia Katarzyna Naliwajko ◽  
Renata Markiewicz-Żukowska ◽  
Krystyna Joanna Gromkowska-Kępka ◽  
Katarzyna Socha ◽  
...  
Keyword(s):  
New Zealand ◽  
Chemical Composition ◽  
Glioblastoma Multiforme ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Cytotoxicity Test ◽  
Diffuse Astrocytoma ◽  
Grade Ii ◽  
Grade Ii Glioma

Abstract Background Several studies have previously reported that propolis and its ingredients inhibit glioma cancer cell lines. The chemical composition and antiproliferative activity of propolis from Poland (PPE) and propolis from New Zealand (MPE) were compared in this study. Methods The chemical composition was investigated by gas chromatography-mass spectrometry. Antiproliferative activity of PPE and MPE was determined by a cytotoxicity test and DNA binding by [ 3 H]-thymidine incorporation on Human Diffuse Astrocytoma cell line (DASC) derived from a patient with a Grade II glioma and glioblastoma multiforme T98G and LN-18 cell lines from American Type Culture Collection. Results The chemical composition of both propolis was comparable, with marginal differences in the amount of some compounds. Flavonoids and chalcones, of which pinocembrin, pinobanksin, pinobanksin 3-acetate, chrysin and galangin showed the highest level, were the main components of both examined propolis (PPE–49.4% and MPE–52.1%). The performed cytotoxicity test showed powerful activity of PPE and MPE propolis on DASC, T98G and LN-18 cells. The degree of the antiproliferative activity was similar in the case of both propolis (viability after 72 h for 30 µg/mL ranged from 22.0% to 51.6% and proliferation inhibition after 72 h approximately was from 18.6% to 75.6%). Conclusions These results are the first to show that propolis from Poland and propolis from New Zealand have a strong cytotoxic and antiproliferative effect on DASC (Grade II glioma) derived from a patient and glioblastoma multiforme T98G and LN-18 cell lines. This activity may be associated with the high content of polyphenolic compounds in both propolis. These findings suggest that Polish and New Zealand propolis shows promising anticancer activity in the treatment of glioblastoma. However, further studies are required.

scholarly journals Chemical composition of Polish propolis and its antiproliferative effect in combination with Bacopa monnieri on glioblastoma cell lines

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Justyna Moskwa ◽  
Sylwia K. Naliwajko ◽  
Renata Markiewicz-Żukowska ◽  
Krystyna J. Gromkowska-Kępka ◽  
Patryk Nowakowski ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Composition ◽  
Cell Lines ◽  
Bacopa Monnieri ◽  
Gas Chromatography Mass Spectrometry ◽  
Array Detector ◽  
Proliferative Potential ◽  
Cytotoxicity Test ◽  
Glioblastoma Cell ◽  
Glioblastoma Cell Lines

AbstractPropolis and Bacopa monnieri (L.) Wettst. (Brahmi) are natural products that contain many active substances and possess anticancer properties. The aim of this study was to investigate the chemical composition of Polish propolis extract (PPE) by gas chromatography-mass spectrometry (GC–MS), B. monnieri extracts (BcH, BcS) by high performance liquid chromatography with diode array detector and mass spectrometry coupled with electrospray ionization (LC–ESI–MS) and finally determine its anti-proliferative potential combined with BcH and BcS in glioblastoma cell lines (T98G, LN-18, U87MG). The antiproliferative activity of PPE, BcH, BcS and their combination (PPE + BcH) was determined by a cytotoxicity test, and DNA binding was determined by [3H]-thymidine incorporation. Flavonoids and phenylpropenoids were the main components of PPE. BcH and BcS samples were also successfully analyzed. Their main constituents were saponins such as bacoside A3, bacopaside II, X and bacopasaponin C and its isomer. The inhibitory effects on the viability and proliferation of the tested glioma cells observed after incubation with the combination of PPE and BcH were significantly stronger than the effects of these two extracts separately. These findings suggest that propolis in combination with B. monnieri shows promising anticancer activity for the treatment of glioblastoma. However, further studies are still required.

scholarly journals Extraction, Chemical Composition, and Anticancer Potential of Origanum onites L. Essential Oil

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2612 ◽  
Author(s):  
Katerina Spyridopoulou ◽  
Eleni Fitsiou ◽  
Eleni Bouloukosta ◽  
Angeliki Tiptiri-Kourpeti ◽  
Manolis Vamvakias ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Biological Activities ◽  
Colorectal Cancer Cell Line ◽  
Ht 29 ◽  
Origanum Onites

Origanum species are plants rich in volatile oils that are mainly used for culinary purposes. In recent years, there has been a growing interest in the biological activities of their essential oils. Origanum onites L. is a plant mainly found in Greece, Turkey, and Sicily, whose oil is rich in carvacrol, a highly bioactive phytochemical. The aim of this study was to analyze the chemical composition of Origanum onites essential oil (OOEO), and investigate its potential anticancer effects in vitro and in vivo. GC/MS analysis identified carvacrol as OOEO’s main constituent. In vitro antiproliferative activity was assayed with the sulforhodamine B (SRB) assay against human cancer cell lines from four tumor types. HT-29, a colorectal cancer cell line, was the most sensitive to the antiproliferative activity of OOEO. Wound-healing assay and Annexin V-PI staining were employed to investigate the antimigratory and the pro-apoptotic potential of OOEO, respectively, against human (HT-29) and murine (CT26) colon cancer cells. Notably, OOEO attenuated migration and induced apoptosis-related morphological changes in both cell lines. Prophylactic oral administration of the oil in a BALB/c experimental mouse model inhibited the growth of syngeneic CT26 colon tumors. As far as we know, this is the first report on the antitumor potential of orally administered OOEO.

Chemical composition, oxidative stability and antiproliferative activity of Anethum graveolens (dill) seed hexane extract

2020 ◽  
Vol 71 (3) ◽  
pp. 374
Author(s):  
I. A. Nehdia ◽  
N. Abutaha ◽  
H. M. Sbihi ◽  
C. P. Tan ◽  
S. I. Al-Resayes
Keyword(s):  
Chemical Composition ◽  
Oxidative Stability ◽  
Antiproliferative Activity ◽  
Lipid Fraction ◽  
Hexane Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Breast Cancer Cell Lines ◽  
Volatile Fraction ◽  
Volatile Components ◽  
Anethum Graveolens

This study aimed to evaluate the physicochemical properties, chemical composition, and anti­proliferative activity of the hexane extract of Anethum graveolens L. (dill) seeds using gas chromatography mass spectrometry (GC-MS). Two fractions, namely the lipid fraction (74%) and volatile fraction (26%), were detected. The extract content of the seeds was about 9.4% and the extract had a pleasant spicy aroma. Oleic acid (52%), cis-vaccenic acid (6.2%), linoleic acid (5.5%), and palmitic acid (3.3%) were the key fatty acids in the lipid fraction, while apiol (23%) and d-carvone (2.4%) were the major volatile components. Tocols (tocopherols and tocotrienols) were also detected in the A. Graveolens extract (155 mg/100 g) and b-tocopherol was identified as the major tocol (71%). Dill extract showed a high oxidative stability (induction time = 45.22 h). Furthermore, dill extract showed antiproliferative activity against breast cancer cell lines.

Sonoran Propolis: Chemical Composition and Antiproliferative Activity on Cancer Cell Lines

Planta Medica ◽  
2007 ◽  
Vol 73 (14) ◽  
pp. 1469-1474 ◽  
Author(s):  
Javier Hernandez ◽  
Francisco Goycoolea ◽  
Jael Quintero ◽  
Ana Acosta ◽  
Marco Castañeda ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Cancer Cell Lines

scholarly journals Antiproliferative, antimicrobial, and antioxidant activity of Lavandula angustifolia Mill. essential oil

2017 ◽  
Vol 7 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Haris Niksic ◽  
Elvira Kovac-Besovic ◽  
Elma Omeragic ◽  
Samija Muratovic ◽  
Jasna Kusturica ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Gas Chromatography Mass Spectrometry ◽  
Lavandula Angustifolia ◽  
Mcf 7

Introduction: We studied the chemical composition and antimicrobial, antioxidant, and antiproliferative activities of essential oils from flowers of Lavandula angustifolia grown in Southern Bosnia and Herzegovina. Methods: The chemical profile of essential oil was evaluated by means of gas chromatography-mass spectrometry. Antimicrobial activity was tested against six bacterial strains. The antioxidant activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) test and the antiproliferative activity against three human cancer cell lines, MCF-7, NCI-H460, and MOLT-4, were investigated using 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide tests. Results: In L. angustifolia essential oil, monoterpene alcohols were the most represented class of volatiles (51.8%), including linalool, lavandulol, and terpinen-4-ol, α-terpineol as the major components, followed by monoterpene esters (22.6%). The most important antibacterial activity of essential oil was expressed on Gram-negative strains. Investigated essential oil was able to reduce DPPH radicals into the neutral DPPH-H form (inhibitory concentration 50% [IC50] = 0.421 mg/ml), and this activity was dose dependent. The essential oil showed significant antiproliferative activity against three cancer cell lines, MOLT-4, MCF-7, and NCI-H460 cells, with IC50 values of 17, 94, and 97 µg/ml, respectively. The result of the antiproliferative assay indicates that MOLT-4 cell line was the most sensitive to investigated essential oil. Conclusion: The results revealed that L. angustifolia essential oil may be important growth inhibitor against the microbes studied. It also possesses significant antioxidant activity and demonstrated excellent antiproliferative activity against MOLT-4 cells.

scholarly journals Essential Oil from Arnica Montana L. Achenes: Chemical Characteristics and Anticancer Activity

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4158 ◽  
Author(s):  
Sugier ◽  
Sugier ◽  
Jakubowicz-Gil ◽  
Winiarczyk ◽  
Kowalski
Keyword(s):  
Chemical Composition ◽  
Glioblastoma Multiforme ◽  
Essential Oil ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Anaplastic Astrocytoma ◽  
Volatile Components ◽  
T98g Cell ◽  
Arnica Montana ◽  
Induction Of Apoptosis

Mountain arnica Arnica montana L. is a source of several metabolite classes with diverse biological activities. The chemical composition of essential oil and its major volatile components in arnica may vary depending on the geographical region, environmental factors, and plant organ. The objective of this study was to characterize the chemical composition of essential oil derived from A. montana achenes and to investigate its effect on induction of apoptosis and autophagy in human anaplastic astrocytoma MOGGCCM and glioblastoma multiforme T98G cell lines. The chemical composition of essential oil extracted from the achenes was examined with the use of Gas Chromatography–Mass Spectrometry GC-MS. Only 16 components of the essential oil obtained from the achenes of 3-year-old plants and 18 components in the essential oil obtained from the achenes of 4-year-old plants constituted ca. 94.14% and 96.38% of the total EO content, respectively. The main components in the EO from the arnica achenes were 2,5-dimethoxy-p-cymene (39.54 and 44.65%), cumene (13.24 and 10.71%), thymol methyl ether (8.66 and 8.63%), 2,6-diisopropylanisole (8.55 and 8.41%), decanal (7.31 and 6.28%), and 1,2,2,3-tetramethylcyclopent-3-enol (4.33 and 2.94%) in the 3- and 4-year-old plants, respectively. The essential oils were found to exert an anticancer effect by induction of cell death in anaplastic astrocytoma and glioblastoma multiforme cells. The induction of apoptosis at a level of 25.7–32.7% facilitates the use of this secondary metabolite in further studies focused on the development of glioma therapy in the future. Probably, this component plays a key role in the anticancer activity against the MOGGCCM and T98G cell lines. The present study is the first report on the composition and anticancer activities of essential oil from A. montana achenes, and further studies are required to explore its potential for future medicinal purposes.

scholarly journals Antimicrobial Activity of Essential Oils againstStreptococcus mutansand their Antiproliferative Effects

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Lívia Câmara de Carvalho Galvão ◽  
Vivian Fernandes Furletti ◽  
Salete Meyre Fernandes Bersan ◽  
Marcos Guilherme da Cunha ◽  
Ana Lúcia Tasca Góis Ruiz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Tumor Cell ◽  
Essential Oils ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Human Tumor ◽  
Gas Chromatography Mass Spectrometry ◽  
Tumor Cell Lines ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines

This study aimed to evaluate the activity of essential oils (EOs) againstStreptococcus mutansbiofilm by chemically characterizing their fractions responsible for biological and antiproliferative activity. Twenty EO were obtained by hydrodistillation and submitted to the antimicrobial assay (minimum inhibitory (MIC) and bactericidal (MBC) concentrations) againstS. mutansUA159. Thin-layer chromatography and gas chromatography/mass spectrometry were used for phytochemical analyses. EOs were selected according to predetermined criteria and fractionated using dry column; the resulting fractions were assessed by MIC and MBC, selected as active fractions, and evaluated againstS. mutansbiofilm. Biofilms formed were examined using scanning electron microscopy. Selected EOs and their selected active fractions were evaluated for their antiproliferative activity against keratinocytes and seven human tumor cell lines. MIC and MBC values obtained for EO and their active fractions showed strong antimicrobial activity. Chemical analyses mainly showed the presence of terpenes. The selected active fractions inhibitedS. mutansbiofilm formation (P<0.05) did not affect glycolytic pH drop and were inactive against keratinocytes, normal cell line. In conclusion, EO showed activity at low concentrations, and their selected active fractions were also effective against biofilm formed byS. mutansand human tumor cell lines.

scholarly journals Chemical composition and cytotoxic activity of the essential oils of Cymbopogon citratus L. Grown in phu tho province

2018 ◽  
Vol 14 (4) ◽  
pp. 683-687 ◽  
Author(s):  
Hoang Thi Kim Van ◽  
Nguyen Minh Quy ◽  
Do Thi Vinh Ha ◽  
Nguyen Thanh Hai ◽  
Hoang Thi Ly ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Hep3b Cell ◽  
Cytotoxicity Test ◽  
Cymbopogon Citratus ◽  
Ic50 Values

Culms and leaves of Cymbopogon citratus L. were collected from two regions of Phu Tho province (Thanh Son and Phu Ninh) and used as materials for essential oil extraction. Oils obtained were steam-distilled, analyzed for chemical composition and evaluated for cytotoxic activity against three different cancer cell lines. The GC/MS analysis showed that citral is the major content of the steam-distilled essential oils which was found in the range of 64.15-76.22%. Camphene was found only in culm oils of both regions but it was not detected in the leaf oils. Interestingly, the isomer forms of ocimene present at higher content in the culm oils than in the leaf oils whereas myrcene content in the leaf oils is higher than that in the culm oils. In a cytotoxicity test, four essential oils of culms and leaves of C. citratus from Thanh Son and Phu Ninh showed potent activity against A549 (human lung carcinoma) cell line with the IC50 values ranging from 4.01±0.39 to 6.3±0.54 µg/ml. The essential oils (culms and leaves) from Phu Ninh exhibited moderate effects on the Hela (human cervical adenocarcinoma) cells with the IC50 values of 19.43±1.16 and 42±2.41 µg/ml, respectively. However, they were inactive against the human hepatocellular carcinoma Hep3B cell line. The essential oils from Thanh Son exhibited potent cytotoxic activity against Hela and Hep3B cell lines with the IC50 values ranging from 1.18±0.26 to 8.91±0.32 µg/ml. The results indicated that the essential oils of C. citratus from Thanh Son, Phu Tho could be considered as a promising candidate for the natural sources of anticancer agents.

scholarly journals Investigation of the effect of steroidal imidazoheterocycles hormone derivatives on JEG-3 trophoblast cells of choriocarcinoma

2021 ◽  
pp. 99-107
Author(s):  
Jovana Todosijević ◽  
Alexander Scherbakov ◽  
Yulia Volkova ◽  
Vladimir Jurišić
Keyword(s):  
Oxygen Atom ◽  
Cell Line ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Chemical Structure ◽  
Biological Activities ◽  
Steroid Hormone ◽  
Cytotoxicity Test ◽  
Trophoblast Cells ◽  
Large Panel

This study aimed to examine the biological activities of unique steroid hormone derivatives-steroidal imidazoheterocycles. The activity synthesized steroidal compounds were tested for the viability of JEG-3 trophoblast cells of choriocarcinoma. The results of the MTT cytotoxicity test showed that most of the examined steroidal imidazoheterocycles act proliferatively on the JEG-3 cell line, except (E, F, and J). Antiproliferative activity of these derivates is probably influenced by different substituents at position 17-C atom in the chemical structure of the hormone and by different substituents on the oxygen atom of acetamide group in the hormone molecule. In order to better confirm the antiproliferative activity of these derivatives, it is necessary to performed complex research on a large panel of cancer cell lines and by different assay.

Synthesis, Characterization, Antiproliferative Activity of Galloyl Derivatives and Investigation of Cytotoxic Properties in HepG2/C3A cells

2021 ◽  
Vol 23 ◽  
Author(s):  
Rafael Claudino dos Santos ◽  
Raquel Oliveira Nascimento de Freitas ◽  
Mary Ann Foglio ◽  
João Ernesto de Carvalho ◽  
Ana Lucia Tasca Góes Ruiz ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Count ◽  
Membrane Permeability ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Membrane Integrity ◽  
Antitumoral Activity ◽  
Cytotoxicity Test ◽  
Significant Activity

Background: Appropriate substituents in the galloyl group could lead to significant biological properties. Objective: Novel galloyl-substituted compounds bearing 2-substituted-1,3,4-oxadiazol-5-yl, 5-substituted-1,2,4-triazol-3-yl, and carboxamide groups were synthesized and evaluated for their antiproliferative activity. Additionally, galloyl hydrazide (2) was evaluated by performing cytotoxicity, membrane integrity, cell cycle, and apoptosis assays in HepG2/C3A cells. Method: General procedure was used for the synthesis of galloyl-substituted (3-9, 11) and characterized by their spectroscopic data (1H and 13C NMR). The antiproliferative activity of all novel galloyl derivatives was evaluated against nine human tumors and one nontumoral cell line. Three response parameters (GI50, TGI, and LC50) were calculated. The cytotoxicity test was performed for the resazurin assay. The membrane integrity, cell cycle, and apoptosis assays were performed by flow cytometry. Results: The substitution of the methoxy group of the galloyl ring system for a carboxamide group (3, 4, 5, and 6) produced compounds with moderate antitumoral activity, particularly 6, against six human cancer cell lines, K-562, PC-3, NCI-ADR/RES, OVCAR, 786-0 and NCI-H460, with GI50 values ≤ 9.45 µg/mL. Triazole derivatives 7 and 8 exhibited higher antitumoral activity toward OVCAR, MCF-7 and leukemia K-562 cell lines, exhibiting GI50 values less than 10 µg/mL. Compound 11 displayed significant activity against PC-3 (GI50 = 4.31 µg/mL), OVCAR (GI50 = 8.84 µg/mL) and K-562 (GI50 = 8.80 µg/mL) cell lines. Galloyl hydrazide (2) had cytotoxic activity in HepG2/C3A cells (IC50 = 153.7 µg/mL). In membrane permeability, cell count, cell cycle, and apoptosis assays, as determined using the IC50 of compound (2) in HepG2/C3A cells, increased membrane permeability, decreased cell count, altered cell cycle, and initial apoptosis was observed compared to the control group. Conclusion: Thus, our results showed for the first time the synthesis, antiproliferative activity, and cytotoxicity of galloyl-substituted compounds. Galloyl-substitution does not have a very strong synergistic effect in the inhibition of cancer cell proliferation compared with galloyl hydrazide (2). Compound 2 demonstrated promising activity in HepG2/C3A hepatocarcinoma cells.

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