scholarly journals Poly(N,N-dimethylacrylamide)-coated upconverting NaYF4:Yb,Er@NaYF4:Nd core–shell nanoparticles for fluorescent labeling of carcinoma cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Viktoriia Oleksa ◽  
Hana Macková ◽  
Hana Engstová ◽  
Vitalii Patsula ◽  
Oleksandr Shapoval ◽  
...  
Keyword(s):  
Upconversion Luminescence ◽  
Polymer Coatings ◽  
Fluorescent Labeling ◽  
Carcinoma Cells ◽  
Cell Labeling ◽  
Infrared Light ◽  
Ultraviolet Region ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Core Shell Nanoparticles

AbstractUpconverting luminescent lanthanide-doped nanoparticles (UCNP) belong to promising new materials that absorb infrared light able to penetrate in the deep tissue level, while emitting photons in the visible or ultraviolet region, which makes them favorable for bioimaging and cell labeling. Here, we have prepared upconverting NaYF4:Yb,Er@NaYF4:Nd core–shell nanoparticles, which were coated with copolymers of N,N-dimethylacrylamide (DMA) and 2-(acryloylamino)-2-methylpropane-1-sulfonic acid (AMPS) or tert-butyl [2-(acryloylamino)ethyl]carbamate (AEC-Boc) with negative or positive charges, respectively. The copolymers were synthesized by a reversible addition-fragmentation chain transfer (RAFT) polymerization, reaching Mn ~ 11 kDa and containing ~ 5 mol% of reactive groups. All copolymers contained bisphosphonate end-groups to be firmly anchored on the surface of NaYF4:Yb,Er@NaYF4:Nd core–shell nanoparticles. To compare properties of polymer coatings, poly(ethylene glycol)-coated and neat UCNP were used as a control. UCNP with various charges were then studied as labels of carcinoma cells, including human hepatocellular carcinoma HepG2, human cervical cancer HeLa, and rat insulinoma INS-1E cells. All the particles proved to be biocompatible (nontoxic); depending on their ξ-potential, the ability to penetrate the cells differed. This ability together with the upconversion luminescence are basic prerequisites for application of particles in photodynamic therapy (PDT) of various tumors, where emission of nanoparticles in visible light range at ~ 650 nm excites photosensitizer.

scholarly journals Poly(N,N-dimethylacrylamide)-Coated Upconverting NaYF4:Yb,Er@NaYF4:Nd Core-Shell Nanoparticles For Fluorescent Labeling of Carcinoma Cells

2021 ◽  
Author(s):  
Viktoriia Oleksa ◽  
Hana Macková ◽  
Hana Engstová ◽  
Vitalii Patsula ◽  
Oleksandr Shapoval ◽  
...  
Keyword(s):  
Upconversion Luminescence ◽  
Polymer Coatings ◽  
Fluorescent Labeling ◽  
Carcinoma Cells ◽  
Cell Labeling ◽  
Infrared Light ◽  
Ultraviolet Region ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Core Shell Nanoparticles

Abstract Upconverting luminescent lanthanide-doped nanoparticles (UCNP) belong to promising new materials that absorb infrared light able to penetrate in the deep tissue level, while emitting photons in the visible or ultraviolet region, which makes them favorable for bioimaging and cell labeling. Here, we have prepared upconverting NaYF4:Yb,Er@NaYF4:Nd core-shell nanoparticles, which were coated with copolymers of N,N-dimethylacrylamide (DMA) and 2-(acryloylamino)-2-methylpropane-1-sulfonic acid (AMPS) or tert-butyl [2-(acryloylamino)ethyl]carbamate (AEC-Boc) with negative or positive charges, respectively. The copolymers were synthesized by a reversible addition-fragmentation chain transfer (RAFT) polymerization, reaching Mn ~11 kDa and containing ~5 mol.% of reactive groups. All copolymers contained bisphosphonate end-groups to be firmly anchored on the surface of NaYF4:Yb,Er@NaYF4:Nd core-shell nanoparticles. To compare properties of polymer coatings, poly(ethylene glycol)-coated and neat UCNP were used as a control. UCNP with various charges were then studied as labels of carcinoma cells, including human hepatocellular carcinoma HepG2, human cervical cancer HeLa, and rat insulinoma INS-1E cells. All the particles proved to be biocompatible (nontoxic); depending on their ξ-potential, the ability to penetrate the cells differed. This ability together with the upconversion luminescence are basic prerequisites for application of particles in photodynamic therapy (PDT) of various tumors, where emission of nanoparticles in visible light range at ~650 nm excites photosensitizer.

One-pot synthesis of ultrasmall β-NaGdF4 nanoparticles with enhanced upconversion luminescence

2019 ◽  
Vol 7 (29) ◽  
pp. 8898-8904 ◽  
Author(s):  
Chunyan Cheng ◽  
Yueshan Xu ◽  
Songtao Liu ◽  
Yuanyuan Liu ◽  
Xian Wang ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Upconversion Luminescence ◽  
Core Shell ◽  
High Quantum Yield ◽  
High Quality ◽  
Shell Nanoparticles ◽  
One Pot ◽  
High Quantum ◽  
One Pot Synthesis ◽  
Core Shell Nanoparticles

High quality β-NaGdYF4:Yb/Er and high quantum yield β-NaGdF4:Yb/Er@β-NaYF4 core shell nanoparticles were synthesized.

On-demand drug release and re-absorption from pirarubicin loaded Fe3O4@ZnO core–shell nanoparticles for targeting infusion chemotherapy for urethral carcinoma

2019 ◽  
Vol 9 (5) ◽  
pp. 467-474 ◽  
Author(s):  
Zhihai Yu ◽  
Yaochun Guo ◽  
Hong Dai ◽  
Baofu Zeng ◽  
Xi Zheng ◽  
...  
Keyword(s):  
Squamous Carcinoma ◽  
Good Choice ◽  
Carcinoma Cells ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Infusion Chemotherapy ◽  
On Demand ◽  
Squamous Carcinoma Cells ◽  
Urethral Carcinoma ◽  
Core Shell Nanoparticles

Although standard treatment recommendations for urethral carcinoma are lacking due to its rare incidence, urethral infusion chemotherapy can be a good choice for urethral carcinoma therapy which minimizes damage to patients compared to radical cystourethrectomy and radiation therapy. In this work, pirarubicin loaded Fe3O4@ZnO core–shell nanoparticles have been successfully synthesized. They can magnetically position in a tubular structure at will and realized on-demand pirarubicin release and re-absorption under UV radiation and darkness. As a result, they can strongly inhibit the human urethral squamous carcinoma cells during infusion chemotherapy and give nearly no damage to normal cells after infusion chemotherapy. Overall, such nanoparticles are highly possible to be a new approach for urethral carcinoma infusion chemotherapy, with satisfactory efficacy and slight side effects.

scholarly journals Synthesis of NaYF4:Yb,Er@SiO2@Ag core-shell nanoparticles for plasmon-enhanced upconversion luminescence in bio-applications

2019 ◽  
Vol 3 (1) ◽  
pp. 013-019
Author(s):  
G Arzumanyan ◽  
D Linnik ◽  
K Mamatkulov ◽  
M Vorobyeva ◽  
A Korsun ◽  
...  
Keyword(s):  
Upconversion Luminescence ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Core Shell Nanoparticles

scholarly journals Bifunctional Tm3+,Yb3+:GdVO4@SiO2 Core-Shell Nanoparticles in HeLa Cells: Upconversion Luminescence Nanothermometry in the First Biological Window and Biolabelling in the Visible

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 993 ◽  
Author(s):  
Oleksandr Savchuk ◽  
Joan Josep Carvajal Marti ◽  
Concepción Cascales ◽  
Patricia Haro-Gonzalez ◽  
Francisco Sanz-Rodríguez ◽  
...  
Keyword(s):  
Hela Cells ◽  
Near Infrared ◽  
Energy Levels ◽  
Upconversion Luminescence ◽  
Biological Tissues ◽  
Visible Range ◽  
Core Shell ◽  
Fluorescence Intensity Ratio ◽  
Shell Nanoparticles ◽  
Core Shell Nanoparticles

The bifunctional possibilities of Tm,Yb:GdVO4@SiO2 core-shell nanoparticles for temperature sensing by using the near-infrared (NIR)-excited upconversion emissions in the first biological window, and biolabeling through the visible emissions they generate, were investigated. The two emission lines located at 700 and 800 nm, that arise from the thermally coupled 3F2,3 and 3H4 energy levels of Tm3+, were used to develop a luminescent thermometer, operating through the Fluorescence Intensity Ratio (FIR) technique, with a very high thermal relative sensitivity. Moreover, since the inert shell surrounding the luminescent active core allows for dispersal of the nanoparticles in water and biological compatible fluids, we investigated the penetration depth that can be realized in biological tissues with their emissions in the NIR range, achieving a value of 0.8 mm when excited at powers of 50 mW. After their internalization in HeLa cells, a low toxicity was observed and the potentiality for biolabelling in the visible range was demonstrated, which facilitated the identification of the location of the nanoparticles inside the cells, and the temperature determination.

scholarly journals Upconversion luminescence and temperature sensing properties of NaGd(WO4)2:Yb3+/Er3+@SiO2 core–shell nanoparticles

RSC Advances ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 3981-3989
Author(s):  
Lu Zheng ◽  
Xinyi Huang ◽  
Jiuping Zhong ◽  
Zijun Wang ◽  
Xiaoning Cheng
Keyword(s):  
Upconversion Luminescence ◽  
Silica Shell ◽  
Temperature Sensing ◽  
Upconversion Nanoparticles ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Sensing Properties ◽  
The Core ◽  
Thermal Sensing ◽  
Core Shell Nanoparticles

A shell of SiO2 with tunable thickness was uniformly coated on NaGd(WO4)2:Yb3+/Er3+ core upconversion nanoparticles (UCNPs). The effects of the silica shell on UC luminescence and thermal sensing properties of the core–shell UCNPs were investigated.

Upconversion luminescence nanoparticles-based lateral flow immunochromatographic assay for cephalexin detection

2014 ◽  
Vol 2 (45) ◽  
pp. 9637-9642 ◽  
Author(s):  
Chunyan Liu ◽  
Wei Ma ◽  
Zhenyu Gao ◽  
Jiayi Huang ◽  
Yi Hou ◽  
...  
Keyword(s):  
Upconversion Luminescence ◽  
Lateral Flow ◽  
Sensitive Detection ◽  
Immunochromatographic Assay ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Core Shell Nanoparticles ◽  
Lateral Flow Immunochromatographic Assay

Upconversion luminescence core–shell nanoparticles were used as antibody labels in a lateral flow immunochromatographic assay for the sensitive detection of cephalexin.

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