Synthesis of no-carrier-added [188, 189, 191Pt]cisplatin from a cyclotron produced 188, 189, 191PtCl42− complex

AbstractWe developed a novel method for production of no-carrier-added (n.c.a.) [188, 189, 191Pt]PtIICl42− from an Ir target material, and then synthesized n.c.a. [*Pt]cis-[PtIICl2(NH3)2] ([*Pt]cisplatin) from [*Pt]PtIICl42−. [*Pt]PtIICl42− was prepared as a synthetic precursor of n.c.a. *Pt complex by a combination of resin extraction and anion-exchange chromatography after the selective reduction of IrIVCl62− with ascorbic acid. The ligand-substitution reaction of Cl with NH3 was promoted by treating n.c.a. [*Pt]PtIICl42− with excess NH3 and heating the reaction mixture, and n.c.a. [*Pt]cisplatin was successfully produced without employing precipitation routes. After this treatment, [*Pt]cisplatin was isolated through preparative HPLC with a radiochemical purity of 99 + % at the end of synthesis (EOS).

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Synthesis of n.c.a. [188, 189, 191pt] Cisplatin From a Cyclotron-produced n.c.a. 188, 189, 191PtCl42- Complex

10.21203/rs.3.rs-123288/v1 ◽

2020 ◽

Author(s):

Honoka Obata ◽

Katsuyuki Minegishi ◽

Kotaro Nagatsu ◽

Mikako Ogawa ◽

Ming Zhang

Keyword(s):

Radiochemical Purity ◽

Selective Reduction ◽

Target Material ◽

Anion Exchange Chromatography ◽

Ligand Substitution ◽

Exchange Chromatography ◽

Preparative Hplc ◽

Pt Complex ◽

Novel Method ◽

Ligand Substitution Reaction

Abstract We developed a novel method for production of no-carrier-added (n.c.a.) [188, 189, 191Pt]PtⅡCl42- from an Ir target material, and then synthesized n.c.a. [*Pt]cis-[PtⅡCl2(NH3)2] ([*Pt]cisplatin) from [*Pt]PtⅡCl42-. [*Pt]PtⅡCl42- was prepared as a synthetic precursor of n.c.a. *Pt complex by a combination of resin extraction and anion-exchange chromatography after the selective reduction of IrⅣCl62- with ascorbic acid. The ligand-substitution reaction of Cl with NH3 was promoted by treating n.c.a. [*Pt]PtⅡCl42- with excess NH3 and heating the reaction mixture, and n.c.a [*Pt]cisplatin was successfully produced without employing precipitation routes. After this treatment, [*Pt]cisplatin was isolated through preparative HPLC with a radiochemical purity of 99+% at the end of synthesis (EOS).

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A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag

Journal of Biomedicine and Biotechnology ◽

10.1155/2009/591923 ◽

2009 ◽

Vol 2009 ◽

pp. 1-8 ◽

Cited By ~ 28

Author(s):

Xudong Wu ◽

Di Wu ◽

Zhisheng Lu ◽

Wentao Chen ◽

Xiaojian Hu ◽

...

Keyword(s):

Catalytic Activity ◽

Large Scale ◽

Fluorescent Protein ◽

Anion Exchange Chromatography ◽

Exchange Chromatography ◽

Scale Production ◽

Sequence Specificity ◽

Tev Protease ◽

Large Scale Production ◽

Novel Method

Because of its stringent sequence specificity, tobacco etch virus (TEV) protease is widely used to remove fusion tags from recombinant proteins. Due to the poor solubility of TEV protease, many strategies have been employed to increase the expression level of this enzyme. In our work, we introduced a novel method to produce TEV protease by using visible superfolder green fluorescent protein (sfGFP) as the fusion tag. The soluble production and catalytic activity of six variants ofsfGFP-TEV was examined, and then the best variant was selected for large-scale production. After purified by Ni-NTA affinity chromatography and Q anion exchange chromatography, the best variant ofsfGFP-TEV fusion protease was obtained with purity of over 98% and yield of over 320 mg per liter culture. ThesfGFP-TEV had a similar catalytic activity to that of the original TEV protease. Our research showed a novel method of large-scale production of visible and functional TEV protease for structural genomics research and other applications.

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Separation of pertechnetate from molybdate by anion-exchange chromatography: Recovery of 99mTc from (n, γ)99Mo and suitability for use in central radiopharmacy (CRPh)

Radiochimica Acta ◽

10.1524/ract.2002.90.7_2002.417 ◽

2002 ◽

Vol 90 (7) ◽

Cited By ~ 12

Author(s):

S. Chattopadhyay ◽

M. K. Das ◽

B. R. Sarkar ◽

Natesan Ramamoorthy

Keyword(s):

Anion Exchange ◽

Anion Exchange Chromatography ◽

Exchange Chromatography ◽

Novel Method

SummaryA novel method for the recovery of

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A simple enzymic method to separate [3H]inositol 1,4,5- and 1,3,4-trisphosphate isomers in tissue extracts

Biochemical Journal ◽

10.1042/bj2600283 ◽

1989 ◽

Vol 260 (1) ◽

pp. 283-286 ◽

Cited By ~ 13

Author(s):

E D Kennedy ◽

I H Batty ◽

E R Chilvers ◽

S R Nahorski

Keyword(s):

Cerebral Cortex ◽

Smooth Muscle ◽

Parotid Gland ◽

Anion Exchange ◽

Anion Exchange Chromatography ◽

Accurate Estimate ◽

Exchange Chromatography ◽

Rat Cerebral Cortex ◽

Tissue Extracts ◽

Novel Method

A novel method to separate [3H]Ins(1,4,5)P3 and [3H]Ins(1,3,4)P3 in tissue extracts is described. It is based on the selective metabolism of Ins(1,3,4)P3 by a crude cerebral supernatant in a Mg2+-free buffer followed by separation of [3H]inositol trisphosphates using conventional anion-exchange chromatography. Evaluation of the assay was performed using [3H]Ins(1,3,4)P3 standards and tissue extracts containing different proportions of [3H]Ins(1,4,5)P3 and [3H]Ins(1,3,4)P3. Parallel h.p.l.c. separations of extracts established the selective and complete metabolism of [3H]Ins(1,3,4)P3 under the above conditions and demonstrated that the enzymic method provides an accurate estimate of the trisphosphate isomers in rat cerebral cortex, parotid gland and bovine tracheal smooth muscle.

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Extracellular polysaccharides from suspension cultures of Nicotiana plumbaginifolia

10.26686/wgtn.12597827.v1 ◽

2020 ◽

Author(s):

Ian Sims ◽

A Bacic

Keyword(s):

Uronic Acid ◽

Cell Suspension Cultures ◽

Anion Exchange Chromatography ◽

Nicotiana Plumbaginifolia ◽

Suspension Cultures ◽

Exchange Chromatography ◽

Arabinogalactan Protein ◽

Extracellular Polysaccharides ◽

Selective Precipitation ◽

The Individual

The soluble polymers secreted by cell-suspension cultures of Nicotiana plumbaginifolia contained 78% carbohydrate, 6% protein and 4% inorganic material. The extracellular polysaccharides were separated into three fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7 and the individual polysaccharides in each fraction were then isolated by selective precipitation and enzymic treatment. Monosaccharide and linkage compositions were determined for each polysaccharide after reduction of uronic acid residues and the degree of esterification of the various uronic acid residues in each polysaccharide was determined concurrently with the linkage types. Six components were identified: an arabinoxyloglucan (comprising 34% of the total polysaccharide) and a galactoglucomannan (15%) in the unbound neutral fraction, a type II arabinogalactan (an arabinogalactan-protein, 11%) and an acidic xylan (3%) in the first bound fraction, and an arabinoglucuronomannan (11%) and a galacturonan (26%) in the second bound fraction. © 1995.

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