scholarly journals Isolation and screening of multifunctional phosphate solubilizing bacteria and its growth-promoting effect on Chinese fir seedlings

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiaqi Chen ◽  
Guangyu Zhao ◽  
Yihui Wei ◽  
Yuhong Dong ◽  
Lingyu Hou ◽  
...  
Keyword(s):  
Plant Growth ◽  
Chinese Fir ◽  
Control Group ◽  
Growth Enhancement ◽  
Phosphate Solubilizing Bacteria ◽  
Total N ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Plant Growth Promoting Traits

AbstractPhosphorus-solubilizing microorganisms is a microbial fertilizer with broad application potential. In this study, 7 endophytic phosphate solubilizing bacteria were screened out from Chinese fir, and were characterized for plant growth-promoting traits. Based on morphological and 16S rRNA sequence analysis, the endophytes were distributed into 5 genera of which belong to Pseudomonas, Burkholderia, Paraburkholderia, Novosphingobium, and Ochrobactrum. HRP2, SSP2 and JRP22 were selected based on their plant growth-promoting traits for evaluation of Chinese fir growth enhancement. The growth parameters of Chinese fir seedlings after inoculation were significantly greater than those of the uninoculated control group. The results showed that PSBs HRP2, SSP2 and JRP22 increased plant height (up to 1.26 times), stem diameter (up to 40.69%) and the biomass of roots, stems and leaves (up to 21.28%, 29.09% and 20.78%) compared to the control. Total N (TN), total P (TP), total K (TK), Mg and Fe contents in leaf were positively affected by PSBs while showed a significant relationship with strain and dilution ratio. The content of TN, TP, TK, available phosphorus (AP) and available potassium (AK) in the soil increased by 0.23–1.12 mg g−1, 0.14–0.26 mg g−1, 0.33–1.92 mg g−1, 5.31–20.56 mg kg−1, 15.37–54.68 mg kg−1, respectively. Treatment with both HRP2, SSP2 and JRP22 increased leaf and root biomass as well as their N, P, K uptake by affecting soil urease and acid phosphatase activities, and the content of available nutrients in soil. In conclusion, PSB could be used as biological agents instead of chemical fertilizers for agroforestry production to reduce environmental pollution and increase the yield of Chinese fir.

scholarly journals Profiling of Plant Growth-Promoting Metabolites by Phosphate-Solubilizing Bacteria in Maize Rhizosphere

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1071
Author(s):  
Minchong Shen ◽  
Jiangang Li ◽  
Yuanhua Dong ◽  
Hong Liu ◽  
Junwei Peng ◽  
...  
Keyword(s):  
Plant Growth ◽  
Microbial Control ◽  
Agricultural Practices ◽  
Phosphate Solubilizing Bacteria ◽  
Mean Values ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Microbial Treatments

Microbial treatment has recently been attracting attention as a sustainable agricultural strategy addressing the current problems caused by unreasonable agricultural practices. However, the mechanism through which microbial inoculants promote plant growth is not well understood. In this study, two phosphate-solubilizing bacteria (PSB) were screened, and their growth-promoting abilities were explored. At day 7 (D7), the lengths of the root and sprout with three microbial treatments, M16, M44, and the combination of M16 and M44 (Com), were significantly greater than those with the non-microbial control, with mean values of 9.08 and 4.73, 7.15 and 4.83, and 13.98 and 5.68 cm, respectively. At day 14 (D14), M16, M44, and Com significantly increased not only the length of the root and sprout but also the underground and aboveground biomass. Differential metabolites were identified, and various amino acids, amino acid derivatives, and other plant growth-regulating molecules were significantly enhanced by the three microbial treatments. The profiling of key metabolites associated with plant growth in different microbial treatments showed consistent results with their performances in the germination experiment, which revealed the metabolic mechanism of plant growth-promoting processes mediated by screened PSB. This study provides a theoretical basis for the application of PSB in sustainable agriculture.

scholarly journals A Bacterium Isolated From Soil in a Karst Rocky Desertification Region Has Efficient Phosphate-Solubilizing and Plant Growth-Promoting Ability

2021 ◽  
Vol 11 ◽  
Author(s):  
Jinge Xie ◽  
Zongqiang Yan ◽  
Guifen Wang ◽  
Wenzhi Xue ◽  
Cong Li ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gluconic Acid ◽  
Lateral Roots ◽  
Utilization Efficiency ◽  
Phosphate Solubilizing Bacteria ◽  
Karst Rocky Desertification ◽  
Rocky Desertification ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

Phosphorus in the soil accessible to plants can easily be combined with calcium ion, the content of which is high in karst rocky desertification (KRD) regions, thereby resulting in a low utilization efficiency of phosphorus. The application of phosphate-solubilizing bacteria (PSB) from the KRD region would facilitate enhanced phosphate availability in the soil. In the present study, the strains belonging to Acinetobacter, Paraburkholderia, and Pseudomonas with efficient phosphate-solubilizing ability were isolated from fruit tree rhizosphere soils in KRD regions. Particularly, Acinetobacter sp. Ac-14 had a sustained and stable phosphate-solubilizing ability (439–448 mg/L, 48–120 h). Calcium carbonate decreased the phosphate-solubilizing ability in liquid medium; however, it did not affect the solubilization index in agar-solidified medium. When cocultivated with Arabidopsis thaliana seedling, Ac-14 increased the number of lateral roots, fresh weight, and chlorophyll content of the seedlings. Metabolomics analysis revealed that Ac-14 could produce 23 types of organic acids, majorly including gluconic acid and D-(-)-quinic acid. Expression of Ac-14 glucose dehydrogenase gene (gcd) conferred Pseudomonas sp. Ps-12 with a sustained and stable phosphate-solubilizing ability, suggesting that the production of gluconic acid is an important mechanism that confers phosphate solubilization in bacteria. Moreover, Ac-14 could also produce indole acetic acid and ammonia. Collectively, the isolated Ac-14 from KRD regions possess an efficient phosphate-solubilizing ability and plant growth-promoting effect which could be exploited for enhancing phosphorus availability in KRD regions. This study holds significance for the improvement of soil fertility and agricultural sustainable development in phosphorus-deficient KRD regions.

Production of Plant Growth Promoting Substances by Phosphate Solubilizing Bacteria Isolated from Vertisols

2007 ◽  
Vol 2 (3) ◽  
pp. 326-333 ◽  
Author(s):  
A. Vikram ◽  
H. Hamzehzarghani . ◽  
A.R. Alagawadi . ◽  
P.U. Krishnaraj . ◽  
B.S. Chandrashekar .
Keyword(s):  
Plant Growth ◽  
Phosphate Solubilizing Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing
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