scholarly journals Leaf morphology and chlorophyll fluorescence characteristics of mulberry seedlings under waterlogging stress

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Liangyi Rao ◽  
Siyuan Li ◽  
Xue Cui
Keyword(s):  
Chlorophyll Fluorescence ◽  
Flooding Tolerance ◽  
Ps Ii ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Chlorophyll Fluorescence Characteristics ◽  
Leaf Chlorophyll ◽  
Fluorescence Characteristics ◽  
Plant Restoration ◽  
Leaf Chlorophyll Fluorescence

AbstractBecause of its high flooding tolerance, in recent years, mulberry has become a tree species that is used in plant restoration in impact zones in reservoir areas. Therefore, 3-year-old potted forage mulberry seedlings were used to investigate the mechanism of mulberry adaptation to flooding stress. An indoor simulated flooding method was adopted to study the morphology of mulberry seedling leaves and the changes in leaf chlorophyll fluorescence parameters and fluorescence imaging under different flooding times and depths. The results showed that the leaves of mulberry seedlings treated with shallow submergence remained healthy during the flooding period, while the leaves of mulberry seedlings treated with half submergence and full submergence showed different degrees of waterlogging symptoms in the middle and late flooding periods and formed adventitious roots at the base of the stem. Most of the chlorophyll fluorescence parameters decreased at the beginning of flooding, but the steady-state degree of closure of PS II reaction centres (1-qP_Lss) increased significantly. In the later stage of flooding, the fluorescence parameters showed relatively stable trends. Based on these results, we conclude that mulberry has high flooding tolerance due to a combination of morphological and physiological responses.

scholarly journals Limitation of photosynthetic processes in photosystem II in alpine mosses exposed to low temperatures: Response of chlorophyll fluorescence parameters

2018 ◽  
Vol 8 (2) ◽  
pp. 218-229 ◽  
Author(s):  
Yeray Folgar Cameán ◽  
Miloš Barták
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
Photosystem Ii ◽  
Temperature Stress ◽  
Low Temperature Stress ◽  
Ps Ii ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Fluorescence Measurements ◽  
Sphagnum Girgensohnii

In this study, we evaluated the effects of low and sub-zero temperature on the fast chlorophyll fluorescence transient (OJIP) and OJIP-derived parameters in 4 different mosses: Sphagnum girgensohnii, Polytrichum formosum, Hylocomium splendens and Pleurozium schreberi. The low temperature stress was applied on the mosses for 90 min. at 3 different temperatures (5°C, -1°C and -10°C). To investigate the effects of this stress on the functioning of photosystem II (PS II), the chlorophyll fluorescence measurements were taken at control temperature (22°C) and, after a 90 min. acclimation period, at each experimental temperature. The shape of OJIP curves and chlorophyll fluorescence parameters were found temperature-dependent in all the species. The mosses differed in their sensitivity to the stress but general trends in response to low temperature were similar. The results support the idea that S. girgensohnii is less resistant to low temperature stress than the other species. We were also interested in the K and L steps in OJIPs, representing different disorders caused by low temperature. The K-step was seen in P. formosum and P. schreberi and the L-step in H. splendens and S. girgensohnii.

The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates

2019 ◽  
Vol 9 (1) ◽  
pp. 37-48
Author(s):  
Alla Orekhova ◽  
Miloš Barták ◽  
Aleyna Özkar ◽  
Josef Elster
Keyword(s):  
Chlorophyll Fluorescence ◽  
Liquid Medium ◽  
Liquid Nitrogen ◽  
Physiological Status ◽  
Ps Ii ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Interspecific Differences ◽  
Subzero Temperatures ◽  
Physiological Properties

In this study, we investigated the effects of shock freezing on physiological properties and consequent growth of in the Antarctic alga Stigeoclonium sp. and comparative coccal alga Diplosphaera chodatii on agar plates. Culture of algae grown in liquid medium were used to study subzero temperatures on the species resistance to shock freezing. Then, microalgae were frozen in liquid nitrogen and inoculated on BBM agar after thawing. Physiological status of algae was evaluated by chlorophyll fluorescence parameters during 28 days. The results showed that interspecific differences existed in their tolerance to shock freezing, as well as their consequent growth rate on agars. Direct effects of freezing in liquid nitrogen was demonstrated in chlorophyll fluorescence parameters recorded immediately after thawing the samples (in liquid medium). In spite of the fact that majority of cells was destroyed by shock freezing, the potential of photochemical processes in PS II (FV/FM) remained constant in D. chodatii. It may indicate high resistance of the species to freezing/thawing cycles and a capability of the surviving cells, core chlorophylls in PS II respectively, to perform photosynthetic processes related to PS II. Contrastingly, Stigeoclonium sp. showed a shock freezing-dependent decrease in FV/FM. When shock-frozen, thawed and inoculated on agar plates, the culture of D. chodatii, and Stigeoclonium sp. showed cultivation time-dependent increase in chlorophyll fluorescence parameters (FV/FM, FS).

Short-term responses of primary processes in PS II to low temperature are sensitively indicated by fast chlorophyll fluorescence kinetics in Antarctic lichen Dermatocarpon polyphyllizum

2017 ◽  
Vol 7 (1) ◽  
pp. 74-82 ◽  
Author(s):  
Michaela Marečková ◽  
Miloš Barták
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
High Energy ◽  
Temperature Decrease ◽  
Temperature Dependent ◽  
Dark Light ◽  
Ps Ii ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Quinone Acceptor

In this study, we investigated the effects of low temperature on the fast chlorophyll fluo-rescence transient (OJIP) and OJIP-derived parameters in chlorolichen Dermatocarpon polyphyllizum expossed to a gradually decreasing temperature (22°C, 18°C, 14°C, 12°C, 10°C, 7°C and 4°C). The segments of lichen thalli were exposed to a certain temperature either in dark- and light-adapted state for 10 minutes in order to evaluate the effects on chlorophyll fluorescence parameters. The initial photochemical phase of the transient (O-J) due to reduction of the primary quinone acceptor (QA) was found temperature dependent. The K-step was apparent for the samples measured at the temperature above 12°C, but not below 10oC in light-adapted lichen thalli. With the thallus temperature decrease, majority of the chlorophyll fluorescence parameters derived from OJIP (ET0/RC, Psi_0, and DI0/RC) showed no change in light-adapted samples but a decrease in dark-adapted samples. The effects of dark- / light-adaptation of the lichen samples on the OJIP and OJIP-derived parameters was attributed to the differences in production/utilization of high-energy products of primary photochemical processes of photosynthesis in dark- and light-adapted state, respectively. The other parameters (ABS/RC, TR0/RC) showed a decrease with thallus temperature decrease both in light- and dark-adapted samples. The results suggest that fast chlorophyll fluorescence trasient is an useful tool to investigate temperature-dependent changes in photosystem II in chlorolichens, their photobionts, respectively.

Effect of Branched Broomrape (Orobanche ramosa) Infection on the Growth and Photosynthesis of Tomato

Weed Science ◽  
2008 ◽  
Vol 56 (4) ◽  
pp. 574-581 ◽  
Author(s):  
Giovanni Mauromicale ◽  
Antonino Lo Monaco ◽  
Angela M. G. Longo
Keyword(s):  
Chlorophyll Fluorescence ◽  
Chlorophyll Content ◽  
Photosynthetic Rate ◽  
Carbon Assimilation ◽  
Parasitic Plants ◽  
Chlorophyll Fluorescence Parameters ◽  
Leaf Chlorophyll Content ◽  
Fluorescence Parameters ◽  
Growing Seasons ◽  
Leaf Chlorophyll

The influence of the holoparasite branched broomrape on the vegetative growth, leaf chlorophyll content, photosynthetic rate, and chlorophyll fluorescence of tomato was studied over two growing seasons on plants grown in a commercial greenhouse. The presence of the parasite strongly reduced the aerial biomass by acting as a competing sink for assimilate, but more importantly, by compromising the efficiency of carbon assimilation via a reduction in leaf chlorophyll content and photosynthetic rate. The chlorophyll fluorescence parameters F0, Fm, Fv, and Fv/Fm were all altered in parasitized plants, indicating that branched broomrape–infected plants are more susceptible to photoinhibition. The degree of damage to the host was not dependent on either the number or the biomass of parasitic plants per host plant. We suggest that the ability to maintain a high photosynthetic rate, leaf chlorophyll content, or both and the ability to minimize photoinhibition can be developed as indirect assays for improved tolerance to branched broomrape.

Features of chlorophyll fluorescence transients can be used to investigate low temperature induced effects on photosystem II of algal lichens from polar regions ( Short Communication )

2015 ◽  
Vol 5 (1) ◽  
pp. 99-111 ◽  
Author(s):  
Anamika Mishra ◽  
Josef Hájek ◽  
Tereza Tuháčková ◽  
Miloš Barták ◽  
Kumud Bandhu Mishra
Keyword(s):  
Chlorophyll Fluorescence ◽  
Lichen Species ◽  
Freezing Stress ◽  
Slow Phase ◽  
Ps Ii ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Species Specific ◽  
Fluorescence Transients ◽  
Chlorophyll Fluorescence Transients

Chlorophyll fluorescence is an effective tool for investigating characteristics of any photosynthesizing organisms and its responses due to different stressors. Here, we have studied a short-term temperature response on three Antarctic green algal lichen species: Umbilicaria antarctica, Xanthoria elegans, and Rhizoplaca melanophtalma. We measured slow chlorophyll fluorescence transients in these Antarctic lichen species during slowely cooling of thallus temperature from 20°C to 5, 0 and -5°C with 20 minute acclimation at each temperature. The measurements were supplemented with saturation pulses for the analysis of chlorophyll fluorescence parameters: maximum yield of PS II photochemistry (FV/FM), effective quantum yield of PS II photochemistry (FPSII) and quenching parameters. In response to decreasing thallus temperature, we observed species-specific changes in chlorophyll fluorescence parameters as well as in the shape of the chlorophyll fluorescence transients. We propose that species-specific changes in the slow phase of chlorophyll fluorescence transients can be potentially used as indicators of freezing stress in photosynthetic apparatus of lichen algal photobionts.

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