Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream

AbstractA customized PCR-array was used for the simultaneous gene expression of the Gh/Igf system and related markers of muscle growth, and lipid and energy metabolism during early life stages of gilthead sea bream (60–127 days posthatching). Also, transcriptional reprogramming by mild hypoxia was assessed in fingerling fish with different history trajectories on O2 availability during the same time window. In normoxic fish, the expression of almost all the genes in the array varied over time with a prompted liver and muscle tissue-specific differentiation, which also revealed temporal changes in the relative expression of markers of the full gilthead sea bream repertoire of Gh receptors, Igfs and Igf-binding proteins. Results supported a different contribution through development of ghr and igf subtypes on the type of action of GH via systemic or direct effects at the local tissue level. This was extensive to Igfbp1/2/4 and Igfbp3/5/6 clades that clearly evolved through development as hepatic and muscle Igfbp subtypes, respectively. This trade-off is however very plastic to cope changes in the environment, and ghr1 and igfbp1/3/4/5 emerged as hypoxic imprinting genes during critical early developmental windows leading to recognize individuals with different history trajectories of oxygen availability and metabolic capabilities later in life.

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Sustained swimming improves muscle growth and cellularity in gilthead sea bream

Journal of Comparative Physiology B ◽

10.1007/s00360-010-0516-4 ◽

2010 ◽

Vol 181 (2) ◽

pp. 209-217 ◽

Cited By ~ 44

Author(s):

Antoni Ibarz ◽

Olga Felip ◽

Jaume Fernández-Borràs ◽

Miguel Martín-Pérez ◽

Josefina Blasco ◽

...

Keyword(s):

Muscle Growth ◽

Gilthead Sea Bream ◽

Sea Bream

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Myomaker and Myomixer Characterization in Gilthead Sea Bream Under Different Myogenesis Conditions.

10.21203/rs.3.rs-1123861/v1 ◽

2021 ◽

Author(s):

Miquel Perelló-Amorós ◽

Aitor Otero-Tarrazón ◽

Violeta Jorge-Pedraza ◽

Isabel García-Pérez ◽

Albert Sánchez-Moya ◽

...

Keyword(s):

Skeletal Muscle ◽

Tissue Injury ◽

Transmembrane Protein ◽

Muscle Growth ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Adult Fish ◽

Post Injury ◽

Genes Expression

Abstract The skeletal muscle is formed by multinucleated myofibers originated by waves of hyperplasia and hypertrophy during myogenesis. Tissue damage triggers a regeneration process including new myogenesis and muscular remodeling. During myogenesis, the fusion of myoblasts is a key step that requires different genes’ expression, including the fusogens Myomaker and Myomixer. The present work aimed to characterize these proteins in gilthead sea bream and their possible role in in vitro myogenesis, at different fish ages and during muscle regeneration after induced tissue injury. Myomaker is a transmembrane protein highly conserved among vertebrates; whereas Myomixer is a micropeptide that is moderately conserved but maintains its crucial AxLyCxL motif. myomaker expression is restricted to skeletal muscle, while the expression of myomixer is more ubiquitous. In primary myocytes culture, myomaker and myomixer expression peaked at day 6 and day 8, respectively. During regeneration, the expression of both fusogens and all the myogenic regulatory factors showed a peak after 16 days post-injury. Moreover, myomaker and myomixer were present at different ages, but in fingerlings there were significantly higher transcript levels than in juveniles or adult fish. Overall, Myomaker and Myomixer are valuable markers of muscle growth that together with other regulatory molecules, can provide a deeper understanding of myogenesis regulation in fish.

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Tissue-specific robustness of fatty acid signatures in cultured gilthead sea bream (Sparus aurata L.) fed practical diets with a combined high replacement of fish meal and fish oil1

Journal of Animal Science ◽

10.2527/jas.2009-2564 ◽

2010 ◽

Vol 88 (5) ◽

pp. 1759-1770 ◽

Cited By ~ 47

Author(s):

L. Benedito-Palos ◽

J. C. Navarro ◽

S. Kaushik ◽

J. Pérez-Sánchez

Keyword(s):

Fatty Acid ◽

Fish Meal ◽

Sparus Aurata ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Tissue Specific

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IGF-I and IGF-II effects on local IGF system and signaling pathways in gilthead sea bream (Sparus aurata) cultured myocytes

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2015.11.011 ◽

2016 ◽

Vol 232 ◽

pp. 7-16 ◽

Cited By ~ 20

Author(s):

Sheida Azizi ◽

Mohammad Ali Nematollahi ◽

Bagher Mojazi Amiri ◽

Emilio J. Vélez ◽

Cristina Salmerón ◽

...

Keyword(s):

Signaling Pathways ◽

Sparus Aurata ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Igf System ◽

Igf I

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Development Temperature Has Persistent Effects on Muscle Growth Responses in Gilthead Sea Bream

PLoS ONE ◽

10.1371/journal.pone.0051884 ◽

2012 ◽

Vol 7 (12) ◽

pp. e51884 ◽

Cited By ~ 31

Author(s):

Daniel Garcia de la serrana ◽

Vera L. A. Vieira ◽

Karl B. Andree ◽

Maria Darias ◽

Alicia Estévez ◽

...

Keyword(s):

Muscle Growth ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Growth Responses ◽

Development Temperature

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Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics

International Journal of Molecular Sciences ◽

10.3390/ijms222313107 ◽

2021 ◽

Vol 22 (23) ◽

pp. 13107

Author(s):

Josefina Blasco ◽

Emilio J. Vélez ◽

Miquel Perelló-Amorós ◽

Sheida Azizi ◽

Encarnación Capilla ◽

...

Keyword(s):

Growth Hormone ◽

White Muscle ◽

Metabolic Response ◽

Sparus Aurata ◽

Muscle Growth ◽

Somatic Growth ◽

Isotopic Analysis ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Bovine Growth Hormone

Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter. An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses. GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation. In white and red muscle reserves, rBGH increased glycogen while reducing lipid. The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect. The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group. Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes. The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream. There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.

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