Tissue-Specific Orchestration of Gilthead Sea Bream Resilience to Hypoxia and High Stocking Density

In teleosts, brain monoamines (dopamine and serotonin) participate in the early response to different acute stressors. However, little is known regarding their role during chronic stress. In a 2 × 2 factorial design, the influence of a high stocking density (HSD) and/or food deprivation (FD) on the brain monoaminergic activity in gilthead sea bream (Sparus aurata) was evaluated. Following a 21-day experimental design, samples from the plasma and brain regions (telencephalon, hypothalamus, and optic tectum) were collected. The dopamine (DA), serotonin (5HT), and their main metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and 5 hydroxyindoleacetic acid (5HIAA), contents were HPLC-assessed in brain tissues, and the ratios DOPAC/DA and 5HIAA/5HT were calculated as indicators of enhanced monoaminergic activity. The plasma levels of cortisol and catecholamine were also evaluated. The cortisol levels increased in fish exposed to HSD and normally fed but, also, in all FD groups, whereas the NA levels decreased in LSD-FD animals. Within the brain, the dopaminergic and serotonergic activities in telencephalon and hypothalamus increased in fish subjected to HSD and in the telencephalon of LSD-FD fish. While DA (hypothalamus) and 5HT (telencephalon) increased in the animals submitted to a HSD, food-deprived fish did not show such an increase. Taken together, our results supported the hypothesis of brain monoaminergic activity participating in maintaining and orchestrating the endocrine response to chronic stress in fish.

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Myrcia sylvatica essential oil in the diet of gilthead sea bream (Sparus aurata L.) attenuates the stress response induced by high stocking density

Aquaculture Nutrition ◽

10.1111/anu.12675 ◽

2018 ◽

Vol 24 (5) ◽

pp. 1381-1392 ◽

Cited By ~ 6

Author(s):

E.M.H. Saccol ◽

Y.A. Parrado-Sanabria ◽

L. Gagliardi ◽

I. Jerez-Cepa ◽

R.H.V. Mourão ◽

...

Keyword(s):

Stress Response ◽

Essential Oil ◽

Sparus Aurata ◽

Stocking Density ◽

Gilthead Sea Bream ◽

Sea Bream

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Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream

Scientific Reports ◽

10.1038/s41598-021-95408-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

F. Naya-Català ◽

P. Simó-Mirabet ◽

J. Calduch-Giner ◽

J. Pérez-Sánchez

Keyword(s):

Time Window ◽

Muscle Growth ◽

Tissue Level ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Tissue Specific ◽

Transcriptional Reprogramming ◽

Igf System ◽

Specific Differentiation ◽

Almost All

AbstractA customized PCR-array was used for the simultaneous gene expression of the Gh/Igf system and related markers of muscle growth, and lipid and energy metabolism during early life stages of gilthead sea bream (60–127 days posthatching). Also, transcriptional reprogramming by mild hypoxia was assessed in fingerling fish with different history trajectories on O2 availability during the same time window. In normoxic fish, the expression of almost all the genes in the array varied over time with a prompted liver and muscle tissue-specific differentiation, which also revealed temporal changes in the relative expression of markers of the full gilthead sea bream repertoire of Gh receptors, Igfs and Igf-binding proteins. Results supported a different contribution through development of ghr and igf subtypes on the type of action of GH via systemic or direct effects at the local tissue level. This was extensive to Igfbp1/2/4 and Igfbp3/5/6 clades that clearly evolved through development as hepatic and muscle Igfbp subtypes, respectively. This trade-off is however very plastic to cope changes in the environment, and ghr1 and igfbp1/3/4/5 emerged as hypoxic imprinting genes during critical early developmental windows leading to recognize individuals with different history trajectories of oxygen availability and metabolic capabilities later in life.

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