Essential role of submandibular lymph node dendritic cells in protective sublingual immunotherapy against murine allergy

AbstractWhile sublingual immunotherapy (SLIT) is known as an allergen-specific treatment for type-1 allergies, how it controls allergic pathogenesis remains unclear. Here, we show the prerequisite role of conventional dendritic cells in submandibular lymph nodes (ManLNs) in the effectiveness of SLIT for the treatment of allergic disorders in mice. Deficiency of conventional dendritic cells or CD4+Foxp3+ regulatory T (Treg) cells abrogates the protective effect of SLIT against allergic disorders. Furthermore, sublingual antigenic application primarily induces antigen-specific CD4+Foxp3+ Treg cells in draining ManLNs, in which it is severely impaired in the absence of cDCs. In ManLNs, migratory CD11b+ cDCs are superior to other conventional dendritic cell subsets for the generation of antigen-specific CD4+Foxp3+ Treg cells, which is reflected by their dominancy in the tolerogenic features to favor this program. Thus, ManLNs are privileged sites in triggering mucosal tolerance mediating protect effect of SLIT on allergic disorders that requires a tolerogenesis of migratory CD11b+ conventional dendritic cells.

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Faculty Opinions recommendation of Essential role of lung plasmacytoid dendritic cells in preventing asthmatic reactions to harmless inhaled antigen.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1020598.232641 ◽

2004 ◽

Author(s):

Ann Hill

Keyword(s):

Dendritic Cells ◽

Essential Role ◽

Plasmacytoid Dendritic Cells

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The Role of Human T-Lymphotropic Virus Type 1 (HTLV-1)-Infected Dendritic Cells in the Development of HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis

Journal of Virology ◽

10.1128/jvi.73.6.4575-4581.1999 ◽

1999 ◽

Vol 73 (6) ◽

pp. 4575-4581 ◽

Cited By ~ 61

Author(s):

Masahiko Makino ◽

Satoshi Shimokubo ◽

Shin-Ichi Wakamatsu ◽

Shuji Izumo ◽

Masanori Baba

Keyword(s):

T Cells ◽

Dendritic Cells ◽

Virus Type ◽

Spastic Paraparesis ◽

Tropical Spastic Paraparesis ◽

Normal Donor ◽

Dependent Manner ◽

T Lymphotropic Virus

ABSTRACT The development of human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is closely associated with the activation of T cells which are HTLV-1 specific but may cross-react with neural antigens (Ags). Immature dendritic cells (DCs), differentiated from normal donor monocytes by using recombinant granulocyte-macrophage colony-stimulating factor and recombinant interleukin-4, were pulsed with HTLV-1 in vitro. The pulsed DCs contained HTLV-1 proviral DNA and expressed HTLV-1 Gag Ag on their surface 6 days after infection. The DCs matured by lipopolysaccharides stimulated autologous CD4+ T cells and CD8+ T cells in a viral dose-dependent manner. However, the proliferation level of CD4+ T cells was five- to sixfold higher than that of CD8+ T cells. In contrast to virus-infected DCs, DCs pulsed with heat-inactivated virions activated only CD4+ T cells. To clarify the role of DCs in HAM/TSP development, monocytes from patients were cultured for 4 days in the presence of the cytokines. The expression of CD86 Ag on DCs was higher and that of CD1a Ag was more down-regulated than in DCs generated from normal monocytes. DCs from two of five patients expressed HTLV-1 Gag Ag. Furthermore, both CD4+ and CD8+ T cells from the patients were greatly stimulated by contact with autologous DCs pulsed with inactivated viral Ag as well as HTLV-1-infected DCs. These results suggest that DCs are susceptible to HTLV-1 infection and that their cognate interaction with T cells may contribute to the development of HAM/TSP.

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An Essential Role of Antigen-Presenting Cell/T-Helper Type 1 Cell-Cell Interactions in Draining Lymph Node during Complete Eradication of Class II–Negative Tumor Tissue by T-Helper Type 1 Cell Therapy

Cancer Research ◽

10.1158/0008-5472.can-05-2246 ◽

2006 ◽

Vol 66 (3) ◽

pp. 1809-1817 ◽

Cited By ~ 31

Author(s):

Kenji Chamoto ◽

Daiko Wakita ◽

Yoshinori Narita ◽

Yue Zhang ◽

Daisuke Noguchi ◽

...

Keyword(s):

Lymph Node ◽

Essential Role ◽

Tumor Tissue ◽

T Helper ◽

Draining Lymph Node ◽

Negative Tumor ◽

Antigen Presenting ◽

Helper Type

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Essential role of Toll-like receptors for dendritic cell and NK1.1+ cell-dependent activation of type 1 immunity by Lactobacillus pentosus strain S-PT84

Immunology Letters ◽

10.1016/j.imlet.2008.06.003 ◽

2008 ◽

Vol 120 (1-2) ◽

pp. 14-19 ◽

Cited By ~ 37

Author(s):

S KOIZUMI ◽

D WAKITA ◽

T SATO ◽

R MITAMURA ◽

T IZUMO ◽

...

Keyword(s):

Dendritic Cell ◽

Essential Role ◽

Toll Like Receptors ◽

Lactobacillus Pentosus

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Role of Langerhans Cells and Other Dendritic Cells in the Pathogenesis of Herpes Simplex Virus Type 1 Infection

Pathogenicity of Human Herpesviruses due to Specific Pathogenicity Genes - Frontiers of Virology ◽

10.1007/978-3-642-85004-2_16 ◽

1994 ◽

pp. 262-280

Author(s):

Eli Sprecher ◽

Yechiel Becker

Keyword(s):

Dendritic Cells ◽

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Langerhans Cells ◽

Simplex Virus

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Prions and the lymphoreticular system

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.2000.0763 ◽

2001 ◽

Vol 356 (1406) ◽

pp. 177-184 ◽

Cited By ~ 40

Author(s):

Charles Weissmann ◽

Alex J. Raeber ◽

Fabio Montrasio ◽

Ivan Hegyi ◽

Rico Frigg ◽

...

Keyword(s):

T Cells ◽

Dendritic Cells ◽

B Lymphocytes ◽

Knockout Mice ◽

Essential Role ◽

Follicular Dendritic Cells ◽

Splenic Lymphocytes ◽

Β Receptor ◽

The Brain

Following intracerebral or peripheral inoculation of mice with scrapie prions, infectivity accumulates first in the spleen and only later in the brain. In the spleen of scrapie–infected mice, prions were found in association with T and B lymphocytes and to a somewhat lesser degree with the stroma, which contains the follicular dendritic cells (FDCs) but not with non–B, non–T cells; strikingly, no infectivity was found in lymphocytes from blood of the same mice. Transgenic PrP knockout mice expressing PrP restricted to either B or T lymphocytes show no prion replication in the lymphoreticular system. Therefore, splenic lymphocytes either acquire prions from another source or replicate them in dependency on other PrP–expressing cells. The essential role of FDCs in prion replication in spleen was shown by treating mice with soluble lymphotoxin–β receptor, which led to disappearance of mature FDCs from the spleen and concomitantly abolished splenic prion accumulation and retarded neuroinvasion following intraperitoneal scrapie inoculation.

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Transformation by Bovine Papillomavirus Type 1 E6 Requires Paxillin

Journal of Virology ◽

10.1128/jvi.02747-07 ◽

2008 ◽

Vol 82 (12) ◽

pp. 5962-5966 ◽

Cited By ~ 17

Author(s):

Ramon Wade ◽

Nicole Brimer ◽

Scott Vande Pol

Keyword(s):

Focal Adhesion ◽

Essential Role ◽

Consensus Sequence ◽

Cellular Protein ◽

Regulatory Proteins ◽

Phosphorylation Sites ◽

Bovine Papillomavirus ◽

Independent Transformation

ABSTRACT Papillomavirus E6 proteins are adapters that change the function of cellular regulatory proteins. The bovine papillomavirus type 1 E6 (BE6) binds to LXXLL peptide sequences termed LD motifs (consensus sequence LDXLLXXL) on the cellular protein paxillin that is a substrate of Src and focal adhesion kinases. Anchorage-independent transformation induced by BE6 required both paxillin and BE6-binding LD motifs on paxillin but was independent of the major tyrosine phosphorylation sites of paxillin. The essential role of paxillin in transformation by BE6 highlights the role of paxillin in the transduction of cellular signals that result in anchorage-independent cell proliferation.

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