scholarly journals Adenovirus detection in Guthrie cards from paediatric leukaemia cases and controls

2008 ◽  
Vol 99 (10) ◽  
pp. 1668-1672 ◽  
Author(s):  
G M Vasconcelos ◽  
M Kang ◽  
M S Pombo-de-Oliveira ◽  
J D Schiffman ◽  
F Lorey ◽  
...  
Keyword(s):  
Paediatric Leukaemia ◽  
Guthrie Cards

Development of a Filter Paper Blood Spot Radioimmunoassay for Thyroid Stimulating Hormone Suitable for a Regional Neonatal Screening Unit

1983 ◽  
Vol 20 (2) ◽  
pp. 93-99 ◽  
Author(s):  
Hilary Moore ◽  
Mary McMillan
Keyword(s):  
Filter Paper ◽  
Neonatal Screening ◽  
Thyroid Stimulating Hormone ◽  
Paper Sample ◽  
Large Numbers ◽  
Critical Separation ◽  
Screening Unit ◽  
Guthrie Cards ◽  
Filter Paper Blood Spot ◽  
Blood Spot

The radioimmunoassay described measures TSH in dried whole blood spots collected from neonates onto filter paper Guthrie cards. Microgranular cellulose is added to the precipitating reagent at the critical separation stage of the assay to overcome imprecision caused by the presence of the filter paper sample disc in the tube. The method was developed for a regional neonatal screening unit and has been found to be very reliable during ten months' routine use. It was required to be as precise, sensitive, accurate, rapid, simple, and inexpensive as possible and suitable for use with automatic diluting equipment in order to process large numbers of samples. Other methods were examined for their suitability and found not to fulfil one or more of the above criteria.

Methanol/acetone treatment helps the amplification of FMR1 CGG repeat fragment in dried blood spots from Guthrie cards

The Lancet ◽  
1999 ◽  
Vol 353 (9159) ◽  
pp. 1153-1154 ◽  
Author(s):  
CJ Hong ◽  
HL Song ◽  
HC Lai ◽  
SJ Tsai ◽  
KJ Hsiao
Keyword(s):  
Dried Blood Spots ◽  
Cgg Repeat ◽  
Blood Spots ◽  
Acetone Treatment ◽  
Guthrie Cards

scholarly journals PO-405 Repositioning existing drugs as novel therapeutics for high-risk paediatric leukaemia

2018 ◽  
Author(s):  
M Karsa ◽  
T Failes ◽  
GM Arndt ◽  
UR Kees ◽  
M Haber ◽  
...  
Keyword(s):  
High Risk ◽  
Novel Therapeutics ◽  
Paediatric Leukaemia

The challenge of obesity in paediatric leukaemia treatment: it is not just size that matters

2014 ◽  
Vol 100 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Jaszianne Tolbert ◽  
Gregory L Kearns
Keyword(s):  
Drug Disposition ◽  
Lymphocytic Leukaemia ◽  
Genetic Constitution ◽  
Acute Lymphocytic Leukaemia ◽  
Dose Individualisation ◽  
Maintenance Of Remission ◽  
Paediatric Leukaemia ◽  
Cure Rates ◽  
The Impact ◽  
Made In

In the last two decades, tremendous advances have been made in the treatment of acute lymphocytic leukaemia (ALL) in children with 5 year ‘cure’ rates in excess of 90%. The maintenance of remission is due, in part, to individualisation of therapy which must consider age, body size, genetic constitution and the impact of disease on drug disposition and action. This review, focused on treatment of ALL and one of the therapeutic mainstays, 6-mercaptopurine, illustrates the importance of obesity as a modulating factor in dose individualisation.

Hereditary hemochromatosis: detection of C282Y and H63D mutations inHFE gene by means of guthrie cards in population of Czech Republic

2002 ◽  
Vol 23 (3) ◽  
pp. 260-263 ◽  
Author(s):  
Marketa Cimburova ◽  
Ivana Putova ◽  
Hana Provaznikova ◽  
Jiri Horak
Keyword(s):  
Czech Republic ◽  
Hereditary Hemochromatosis ◽  
Guthrie Cards

Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards

2020 ◽  
Author(s):  
Samileh Noorbakhsh ◽  
Mohammad Farhadi ◽  
Faezeh Haghighi ◽  
Sara Minaeian ◽  
Morteza Haghighi Hasanabad
Keyword(s):  
Early Stage ◽  
Limited Data ◽  
Cmv Infection ◽  
Congenital Cytomegalovirus ◽  
Congenital Cmv Infection ◽  
Effective Counseling ◽  
Total Dna ◽  
Guthrie Cards ◽  
Congenital Cmv

Background and Objectives: Cytomegalovirus (CMV) constitutes the most common viral cause of congenital infections in newborns worldwide. There are a significant number of asymptomatic newborns with congenital CMV infection in Iran, which may develop long-term sequelae of infection. Unfortunately, limited data exsists from Iran on the rate of congenital CMV infection among neonates. The current study was aimed to investigate the prevalence of congenital CMV infection among Iranian neonates by testing Guthrie cards. Materials and Methods: Guthrie cards were collected from infants within 2 weeks of life, and total DNA was extracted from samples by thermal shock and evaluated for CMV DNA using nested-PCR assay. CMV infection in newborns was confirmed through a commercial CMV PCR kit. Infected infants underwent further evaluation at the hospital. Results: CMV infection was identified in four of 1174 infants (0.34%) which is approximately 3 cases per 1000 live births. Infected infants were asymptomatic at birth and had a normal hearing status similar to other children. There were no factors in relation with CMV infection among newborns. Conclusion: According to the results of this study, infected infants with congenital CMV infection could identify at early stage by testing Guthrie cards (within 21 days of life). Furthermore, since there is a lack of CMV knowledge in our popula- tion, educating and effective counseling by obstetricians/ gynecologists to the pregnant women are recommended.  

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