Hormone sensitive lipase expression and adipose tissue metabolism show gender difference in obese subjects after weight loss

2002 ◽  
Vol 26 (1) ◽  
pp. 6-16 ◽  
Author(s):  
M Kolehmainen ◽  
H Vidal ◽  
JJ Ohisalo ◽  
E Pirinen ◽  
E Alhava ◽  
...  
Keyword(s):  
Weight Loss ◽  
Adipose Tissue ◽  
Gender Difference ◽  
Hormone Sensitive Lipase ◽  
Adipose Tissue Metabolism ◽  
Tissue Metabolism ◽  
Hormone Sensitive ◽  
Obese Subjects ◽  
Lipase Expression

Adipose tissue metabolism in the postprandial period: microdialysis and arteriovenous techniques compared

1998 ◽  
Vol 274 (4) ◽  
pp. E651-E655 ◽  
Author(s):  
Lucinda K. M. Summers ◽  
Peter Arner ◽  
Vera Ilic ◽  
Mo L. Clark ◽  
Sandy M. Humphreys ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Hormone Sensitive Lipase ◽  
Glycerol Release ◽  
Postprandial Period ◽  
Adipose Tissue Metabolism ◽  
Tissue Metabolism ◽  
Hormone Sensitive ◽  
The Difference ◽  
Arteriovenous Difference

We investigated whether two different methods of studying metabolism in adipose tissue, microdialysis and the arteriovenous technique, produced comparable results during the postprandial period. Interstitial glycerol concentrations measured by microdialysis are usually used as an index of intracellular lipolysis, and it is not known whether they also reflect the intravascular action of lipoprotein lipase in the postprandial period. The two techniques were compared in 10 healthy subjects fed mixed meals. Interstitial glycerol concentrations reflected those measured in adipose tissue venous plasma. However, the calculation of the rate of glycerol release from adipose tissue using the microdialysis data differed systematically from that using arteriovenous difference measurement. The former method gave, on average, 40% lower values than the latter one. The difference is probably due to the assumptions that had to be made for the calculation of glycerol release. The two techniques have complementary places in the study of postprandial adipose tissue metabolism, with microdialysis reflecting intracellular hormone-sensitive lipase action rather than intravascular lipoprotein lipase.

scholarly journals Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese Humans

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Shakun Karki ◽  
Melissa G. Farb ◽  
Samantha Myers ◽  
Caroline Apovian ◽  
Donald T. Hess ◽  
...  
Keyword(s):  
Weight Loss ◽  
Adipose Tissue ◽  
Disease Risk ◽  
Cardiovascular Disease Risk ◽  
Human Adipose Tissue ◽  
Hormone Sensitive Lipase ◽  
Triglyceride Hydrolysis ◽  
Hormone Sensitive ◽  
Obese Subjects ◽  
Subcutaneous Adipose

Background.Dysregulated lipolysis has been implicated in mechanisms of cardiometabolic disease and inflammation in obesity.Purpose. We sought to examine the effect of bariatric weight loss on adipose tissue lipolytic gene expression and their relationship to systemic metabolic parameters in obese subjects.Methods/Results. We biopsied subcutaneous adipose tissue in 19 obese individuals (BMI 42 ± 5 kg/m2, 79% female) at baseline and after a mean period of 8 ± 5 months (range 3–15 months) following bariatric surgery. We performed adipose tissue mRNA expression of proteins involved in triglyceride hydrolysis and correlated their weight loss induced alterations with systemic parameters associated with cardiovascular disease risk. mRNA transcripts of adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and lipid droplet proteins comparative gene identification 58 (CGI-58) and perilipin increased significantly after weight loss (p<0.05for all). ATGL expression correlated inversely with plasma triglyceride (TG), hemoglobin A1C (HbA1C), and glucose, and HSL expression correlated negatively with glucose, while CGI-58 was inversely associated with HbA1C.Conclusion. We observed increased expression of adipose tissue lipolytic genes following bariatric weight loss which correlated inversely with systemic markers of lipid and glucose metabolism. Functional alterations in lipolysis in human adipose tissue may play a role in shaping cardiometabolic phenotypes in human obesity.

Endurance training changes in lipolytic responsiveness of obese adipose tissue

1998 ◽  
Vol 275 (6) ◽  
pp. E951-E956 ◽  
Author(s):  
I. De Glisezinski ◽  
F. Crampes ◽  
I. Harant ◽  
M. Berlan ◽  
J. Hejnova ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Physical Training ◽  
The Body ◽  
Hormone Sensitive Lipase ◽  
Aerobic Exercise Training ◽  
Adrenoceptor Agonist ◽  
Before And After ◽  
Hormone Sensitive ◽  
Obese Subjects

The aim of this study was to investigate the effect of aerobic exercise training on the lipolytic response of adipose tissue in obese subjects. Thirteen men (body mass index = 36.9 ± 1.3 kg/m2) were submitted to aerobic physical training on a cycloergometer (30–45 min, 4 days a wk) for 3 mo. Adipocyte sensitivity to the action of catecholamines and insulin was studied in vitro before and after training. Training induced a decrease in the percentage of fat mass ( P < 0.05) without changing the body weight. Basal lipolysis and hormone-sensitive lipase activity were significantly decreased after training ( P < 0.05). The lipolytic effects of epinephrine, isoprenaline (β-adrenoceptor agonist), and dobutamine (β1-adrenoceptor agonist) were significantly increased ( P < 0.05) but not those of procaterol (β2-adrenoceptor agonist). The antilipolytic effects of α2-adrenoceptor and insulin were significantly decreased ( P < 0.05). Lipolysis stimulation by agents acting at the postreceptor level was unchanged after training. In conclusion, aerobic physical training in obese male subjects modifies adipose tissue lipolysis through an enhancement of β-adrenergic response and a concomitant blunting of adipocyte antilipolytic activity.

Weight loss and regain in obese individuals: a link with adipose tissue metabolism indices?

2013 ◽  
Vol 69 (3) ◽  
pp. 497-505 ◽  
Author(s):  
P. Mauriège ◽  
P. Imbeault ◽  
E. Doucet ◽  
M. Lacaille ◽  
D. Langin ◽  
...  
Keyword(s):  
Weight Loss ◽  
Adipose Tissue ◽  
Adipose Tissue Metabolism ◽  
Tissue Metabolism
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