scholarly journals HPTLC-aptastaining – Innovative protein detection system for high-performance thin-layer chromatography

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Lena Morschheuser ◽  
Hauke Wessels ◽  
Christina Pille ◽  
Judith Fischer ◽  
Tim Hünniger ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Detection System ◽  
Protein Detection ◽  
Layer Chromatography

scholarly journals A validated quantification of triclosan in toothpaste using high-performance thin-layer chromatography and a 48-bit fatbed scanner

2021 ◽  
Author(s):  
Barbara Anders ◽  
Sabrina Doll ◽  
Bernd Spangenberg
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Detection System ◽  
Flatbed Scanner ◽  
Butyl Ether ◽  
Quantification Method ◽  
Direct Measurements ◽  
Layer Chromatography

AbstractWe present a densitometric quantification method for triclosan in toothpaste, separated by high-performance thin-layer chromatography (HPTLC) and using a 48-bit flatbed scanner as the detection system. The sample was band-wise applied to HPTLC plates (10 × 20 cm), with fluorescent dye, Merck, Germany (1.05554). The plates were developed in a vertical developing chamber with 20 min of chamber saturation over 70 mm, using n-heptane–methyl tert-butyl ether–acetic acid (92:8:0.1, V/V) as solvent. The RF value of triclosan is hRF = 22.4, and quantification is based on direct measurements using an inexpensive 48-bit flatbed scanner for color measurements (in red, green, and blue) after plate staining with 2,6-dichloroquinone-4-chloroimide (Gibbs' reagent). Evaluation of the red channel makes the measurements of triclosan very specific. For linearization, an extended Kubelka–Munk expression was used for data transformation. The range of linearity covers more than two orders of magnitude and is between 91 and 1000 ng. The separation method is inexpensive, fast and reliable.

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