New structural insight of C-terminal region of Syntenin-1, enhancing the molecular dimerization and inhibitory function related on Syndecan-4 signaling

SARS coronavirus ORF6 inhibits the classical nuclear import pathway to antagonize host antiviral responses. Several models were proposed to explain its inhibitory function, but quantitative measurement is needed for model evaluation and refinement. We report a broadly applicable live-cell method for calibrated dose-response characterization of the nuclear transport alteration by a protein of interest. Using this method, we found that SARS-CoV-2 ORF6 is ~5 times more potent than SARS-CoV-1 ORF6 in inhibiting bidirectional nuclear transport, due to differences in the NUP98-binding C-terminal region that is required for the inhibition. The N-terminal region was also required, but its membrane binding function was dispensable, since loss of the inhibitory function due to N-terminal truncation was rescued by forced oligomerization using a soluble construct. Based on these data, we propose that the hydrophobic N-terminal region drives oligomerization of ORF6 to multivalently cross-link the FG domains of NUP98 at the nuclear pore complex.

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Identification, N-terminal region sequencing and similarity analysis of differentially expressed proteins in Paracoccidioides brasiliensis

Medical Mycology ◽

10.1046/j.1365-280x.1999.00211.x ◽

1999 ◽

Vol 37 (2) ◽

pp. 115-121 ◽

Cited By ~ 2

Author(s):

A. F. Cunha ◽

M. V. Sousa ◽

S. P. Silva ◽

R. S. A. JesuIno ◽

C. M. A. Soares ◽

...

Keyword(s):

Paracoccidioides Brasiliensis ◽

Terminal Region ◽

Differentially Expressed ◽

Similarity Analysis ◽

Differentially Expressed Proteins

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Antibodies against the COOH-terminal region of E. coli ClpP protease in patients with primary biliary cirrhosis

Journal of Hepatology ◽

10.1034/j.1600-0641.2000.033004528.x ◽

2000 ◽

Vol 33 (4) ◽

pp. 528-536 ◽

Cited By ~ 9

Author(s):

Isabel Mayo ◽

Paz Arizti ◽

Albert Pares ◽

Joaquin Oliva ◽

Rita Alvarez Doforno ◽

...

Keyword(s):

Primary Biliary Cirrhosis ◽

Terminal Region ◽

Biliary Cirrhosis ◽

E Coli

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Inhibitory Function in Auditory Processing

10.3389/978-2-88919-667-8 ◽

2015 ◽

Keyword(s):

Auditory Processing ◽

Inhibitory Function

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Comparing the transfer effects of three nonpharmacological interventions in children with AD/HD: a single-case experimental design

10.31234/osf.io/xstjz ◽

2020 ◽

Author(s):

Da-Wei Zhang ◽

Stuart J. Johnstone ◽

Hui Li ◽

Xiangsheng Li ◽

Li Sun

Keyword(s):

Experimental Design ◽

Visual Analysis ◽

Single Case ◽

Multiple Baseline ◽

Neurofeedback Training ◽

Transfer Effects ◽

Baseline Phase ◽

Intervention Phase ◽

Inhibitory Function ◽

Home Training

The current study used behavioral and electroencephalograph measures to compare the transferability of cognitive training (CT), neurofeedback training (NFT), and CT combined with NFT in children with AD/HD. Following a multiple-baseline single-case experimental design, twelve children were randomized to a training condition. Each child completed a baseline phase, followed by an intervention phase. The intervention phase consisted of 20 sessions of at-home training. Tau-U analysis and standardized visual analysis were adopted to detect effects. CT improved inhibitory function, and NFT showed improved alpha activity and working memory. The combined condition, who was a reduced 'dose' of CT and NFT, did not show any improvements. The three conditions did not alleviate AD/HD symptoms. While CT and NFT may have near transfer effects, considering the lack of improvement in symptoms, this study does not support CT and NFT on their own as a treatment for children with AD/HD.

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