Background:
Jingzhiguanxin (JZGX) tablet, a traditional Chinese prescription, is commonly
used for treating coronary heart disease and angina pectoris in the clinic. There are six active components
(Danshensu (DSS), Protocatechuic aldehyde (PD), Paeoniflorin (PF), Ferulic acid (FA), Salvianolic
acid B (Sal B) and Tanshinone IIA (TA)) in JZGX tablet.
</P><P>
Objective: In this paper, a simple and reliable method was used for simultaneous determining the six
active components by high-performance liquid chromatography coupled with diode array detector
(HPLC-DAD).
Methods:
These six active components were separated on an Agilent Zorbax Eclipse XDB-C18 column
(150 mmx4.6 mm, 5 µm) at 30 °C. Acetonitrile (A), methanol (B) and 0.5% H3PO4 aqueous solution
(C) were used as mobile phase for gradient elution. The flow rate was 1 mL/min and the detection
wavelengths were set at 280 nm for DSS, PD and Sal B, 230 nm for PF, 320 nm for FA and 270 nm for
TA, respectively.
Results:
All of the six components showed good linearity regressions (r2≥0.9997) in the detected concentration
range. The recovery rates and coefficient of variation (CV) for all analytes were 98.66%-
100.18% and 0.75%-1.89%, respectively. This method was successfully applied to simultaneously determine
the six components in JZGX tablet from different batches and manufacturers.
Conclusion:
The validated method can be used in routine quality control analysis of JZGX tablet without
any interference.
