A comparative study on ganglioside micelles using electronic energy transfer, fluorescence correlation spectroscopy and light scattering techniques

2009 ◽  
Vol 11 (21) ◽  
pp. 4335 ◽  
Author(s):  
Radek Šachl ◽  
Ilya Mikhalyov ◽  
Martin Hof ◽  
Lennart B. Å. Johansson
Keyword(s):  
Energy Transfer ◽  
Light Scattering ◽  
Comparative Study ◽  
Fluorescence Correlation Spectroscopy ◽  
Electronic Energy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Electronic Energy Transfer

scholarly journals Optical detection of gadolinium(iii) ions via quantum dot aggregation

RSC Advances ◽  
2017 ◽  
Vol 7 (40) ◽  
pp. 24730-24735 ◽  
Author(s):  
Steven D. Quinn ◽  
Steven W. Magennis
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Quantum Dot ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Trivalent Metal ◽  
Fluorescence Correlation ◽  
Trivalent Metal Ions ◽  
Cdte Quantum Dot ◽  
Fluorescence Dynamic

CdTe quantum dot aggregation induced by trivalent metal ions is followed using fluorescence, dynamic light scattering and fluorescence correlation spectroscopy.

scholarly journals Characterizing nanoparticles in complex biological media and physiological fluids with depolarized dynamic light scattering

Nanoscale ◽  
2015 ◽  
Vol 7 (14) ◽  
pp. 5991-5997 ◽  
Author(s):  
S. Balog ◽  
L. Rodriguez-Lorenzo ◽  
C. A. Monnier ◽  
M. Obiols-Rabasa ◽  
B. Rothen-Rutishauser ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Fluorescence Correlation Spectroscopy ◽  
Biological Fluids ◽  
Correlation Spectroscopy ◽  
Biological Media ◽  
Fluorescence Correlation ◽  
Depolarized Scattering

Characterization of NPs in physiological and biological fluids is challenging, yet can be met by using depolarized scattering, offering advantages over UV-Vis and fluorescence correlation spectroscopy.

scholarly journals Dark States in Ionic Oligothiophene Bioprobes—Evidence from Fluorescence Correlation Spectroscopy and Dynamic Light Scattering

2014 ◽  
Vol 118 (22) ◽  
pp. 5924-5933 ◽  
Author(s):  
Heike Hevekerl ◽  
Jens Wigenius ◽  
Gustav Persson ◽  
Olle Inganäs ◽  
Jerker Widengren
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

Evidence that GPVI is Expressed as a Mixture of Monomers and Dimers, and that the D2 Domain is not Essential for GPVI Activation

2021 ◽  
Author(s):  
Joanne C. Clark ◽  
Raluca A. I. Neagoe ◽  
Malou Zuidscherwoude ◽  
Deirdre M. Kavanagh ◽  
Alexandre Slater ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Fluorescence Correlation Spectroscopy ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Glycoprotein Vi ◽  
D2 Domain ◽  
Microscopy Techniques

AbstractCollagen has been proposed to bind to a unique epitope in dimeric glycoprotein VI (GPVI) and the number of GPVI dimers has been reported to increase upon platelet activation. However, in contrast, the crystal structure of GPVI in complex with collagen-related peptide (CRP) showed binding distinct from the site of dimerization. Further fibrinogen has been reported to bind to monomeric but not dimeric GPVI. In the present study, we have used the advanced fluorescence microscopy techniques of single-molecule microscopy, fluorescence correlation spectroscopy (FCS) and bioluminescence resonance energy transfer (BRET), and mutagenesis studies in a transfected cell line model to show that GPVI is expressed as a mixture of monomers and dimers and that dimerization through the D2 domain is not critical for activation. As many of these techniques cannot be applied to platelets to resolve this issue, due to the high density of GPVI and its anucleate nature, we used Förster resonance energy transfer (FRET) to show that endogenous GPVI is at least partially expressed as a dimer on resting and activated platelet membranes. We propose that GPVI may be expressed as a monomer on the cell surface and it forms dimers in the membrane through diffusion, giving rise to a mixture of monomers and dimers. We speculate that the formation of dimers facilitates ligand binding through avidity.

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