Simultaneous separation and detection of cations and anions on a microfluidic device with suppressed electroosmotic flow and a single injection point

The Analyst ◽  
2010 ◽  
Vol 135 (6) ◽  
pp. 1351 ◽  
Author(s):  
Brent R. Reschke ◽  
Jarrod Schiffbauer ◽  
Boyd F. Edwards ◽  
Aaron T. Timperman
Keyword(s):  
Microfluidic Device ◽  
Electroosmotic Flow ◽  
Single Injection ◽  
Injection Point ◽  
Simultaneous Separation

scholarly journals Electroosmotic flow driven microfluidic device for bacteria isolation using magnetic microbeads

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Samuel Miller ◽  
Alison A. Weiss ◽  
William R. Heineman ◽  
Rupak K. Banerjee
Keyword(s):  
Microfluidic Device ◽  
Electroosmotic Flow ◽  
Point Of Care ◽  
Food Poisoning ◽  
Fluorescent Intensity ◽  
Magnetic Microbeads ◽  
E Coli ◽  
Detection Assay ◽  
Flow Capture ◽  
Improved Performance

Abstract The presence of bacterial pathogens in water can lead to severe complications such as infection and food poisoning. This research proposes a point-of-care electroosmotic flow driven microfluidic device for rapid isolation and detection of E. coli in buffered solution (phosphate buffered saline solution). Fluorescent E. coli bound to magnetic microbeads were driven through the microfluidic device using both constant forward flow and periodic flow switching at concentrations ranging from 2 × 105 to 4 × 107 bacteria/mL. A calibration curve of fluorescent intensity as a function of bacteria concentration was created using both constant and switching flow, showing an increase in captured fluorescent pixel count as concentration increases. In addition, the use of the flow switching resulted in a significant increase in the capture efficiency of E. coli, with capture efficiencies up to 83% ± 8% as compared to the constant flow capture efficiencies (up to 39% ± 11%), with a sample size of 3 µL. These results demonstrate the improved performance associated with the use of the electroosmotic flow switching system in a point-of-care bacterial detection assay.

Amplification-free detection of DNA in a paper-based microfluidic device using electroosmotically balanced isotachophoresis

Lab on a Chip ◽  
2018 ◽  
Vol 18 (6) ◽  
pp. 861-868 ◽  
Author(s):  
Tally Rosenfeld ◽  
Moran Bercovici
Keyword(s):  
Microfluidic Device ◽  
Electroosmotic Flow ◽  
Analytical Device

Microfluidic paper-based analytical device that utilizes the native high electroosmotic flow in nitrocellulose to achieve stationary isotachophoresis focusing.

Safety and efficacy of administering abobotulinumtoxinA through a single injection point when treating lateral periocular rhytides

2011 ◽  
Vol 10 (3) ◽  
pp. 232-234 ◽  
Author(s):  
Monika G Kiripolsky ◽  
Mitchel P Goldman
Keyword(s):  
Single Injection ◽  
Injection Point ◽  
Safety And Efficacy

scholarly journals Pressure management in smart gas networks for increasing hydrogen blending

2022 ◽  
Vol 334 ◽  
pp. 03003
Author(s):  
Marco Cavana ◽  
Enrico Vaccariello ◽  
Pierluigi Leone
Keyword(s):  
Asset Management ◽  
Distribution Systems ◽  
Single Injection ◽  
Fluid Dynamic ◽  
Pressure Management ◽  
Hydrogen Distribution ◽  
Injection Point ◽  
Gas Networks ◽  
Management Schemes

The injection of hydrogen into existing gas grids is acknowledged as a promising option for decarbonizing gas systems and enhancing the integration among energy sectors. Nevertheless, it affects the hydraulics and the quality management of networks. When the network is fed by multiple infeed sites and hydrogen is fed from a single injection point, non-homogeneous hydrogen distribution throughout the grid happens to lead to a reduction of the possible amount of hydrogen to be safely injected within the grid. To mitigate these impacts, novel operational schemes should therefore be implemented. In the present work, the modulation of the outlet pressures of gas infeed sites is proposed as an effective strategy to accommodate larger hydrogen volumes into gas grids, extending the area of the network reached by hydrogen while keeping compliance with quality and hydraulic restrictions. A distribution network operated at two cascading pressure tiers interfaced by pressure regulators constitutes the case study, which is simulated by a fluid-dynamic and multi-component model for gas networks. Results suggest that higher shares of hydrogen and other green gases can be introduced into existing distribution systems by implementing novel asset management schemes with negligible impact on grid operations.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

Quantifying in vivo dopaminergic D2-receptors Bmax and KdVr with microPET® focus after a single injection of [11C]raclopride

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S649-S649
Author(s):  
Laurent Besret ◽  
Jean-Dominique Gallezot ◽  
Frédéric Dollé ◽  
Philippe Hantraye ◽  
Marie-Claude Grégoire
Keyword(s):  
Single Injection ◽  
D2 Receptors ◽  
Quantifying In
Sign in / Sign up

Export Citation Format

Share Document