A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing

Lab on a Chip ◽  
2012 ◽  
Vol 12 (24) ◽  
pp. 5155 ◽  
Author(s):  
Yuriko Oyama ◽  
Toshihisa Osaki ◽  
Koki Kamiya ◽  
Ryuji Kawano ◽  
Tsutomu Honjoh ◽  
...  
Keyword(s):  
Glass Fiber ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Point Of Care Testing ◽  
Fiber Sheet

scholarly journals Point of care testing evaluation of lateral flow immunoassay for diagnosis of Cryptococcus Meningitis in Kenya, 2017

2019 ◽  
Author(s):  
Lawrence Kirimi Gitonga ◽  
Waqo Gufu Boru ◽  
Arthur Kwena ◽  
Marybeth Maritim ◽  
Joyce Wamicwe ◽  
...  
Keyword(s):  
Point Of Care ◽  
Cross Sectional Study ◽  
Latex Agglutination ◽  
Capillary Blood ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Point Of Care Testing ◽  
Cross Sectional ◽  
India Ink ◽  
Cryptococcus Meningitis

Abstract Objectives: The objective of this study was to evaluate the performance of lateral flow immunoassay (LFA) against latex agglutination (LA), India ink and culture in point-of-care diagnosis of CM. We conducted a cross-sectional study among patients with suspected CM at Mbagathi Hospital, Nairobi, April-July 2017. Results: Of 124 capillary blood and serum and 99 cerebrospinal fluid (CSF) samples, the agreement between LFA and LA on serum was 94.4%, kappa (0.88), sensitivity (100%) and specificity (91%). LFA and LA on CSF, was 97.9%, kappa (0.96), sensitivity (100%) and specificity (96%). LFA and India ink was 96.9%, kappa (0.94), sensitivity (100%) and specificity (94.1%). On CSF culture, the agreement was 72.7%, kappa (0.43), sensitivity (100%) and specificity (64%). The agreement of LFA on capillary blood, serum and CSF was 100% with kappa (1.00), sensitivity and specificity of 100%

scholarly journals The Application of Lateral Flow Immunoassay in Point of Care Testing: A Review

2016 ◽  
Vol 8 (3) ◽  
Author(s):  
Kan Wang ◽  
Weijian Qin ◽  
Yafei Hou ◽  
Kun Xiao ◽  
Wenqiang Yan
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Point Of Care Testing

scholarly journals Lower Urine Tenofovir Concentrations among Individuals Taking Tenofovir Alafenamide versus Tenofovir Disoproxil Fumarate: Implications for Point-of-Care Testing

2021 ◽  
Author(s):  
Kelly A Johnson ◽  
Xin Niu ◽  
David V Glidden ◽  
Jose R Castillo-Mancilla ◽  
Jenna Yager ◽  
...  
Keyword(s):  
Tenofovir Disoproxil Fumarate ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Directly Observed Therapy ◽  
Point Of Care Testing ◽  
Separate Point ◽  
Tenofovir Alafenamide ◽  
Adherence Monitoring ◽  
Therapy Studies

Abstract From directly-observed-therapy studies, urine tenofovir (TFV) levels were 74% lower when taking tenofovir alafenamide (TAF) versus tenofovir disoproxil fumarate. Urine TFV remains quantifiable across a range of TAF adherence patterns, but a separate point-of-care lateral flow immunoassay with a lower TFV threshold will be needed to support TAF adherence monitoring.

scholarly journals Experiments And Simulations of Thermometric Lateral Flow Immunoassay For Point-of-Care Testing

2021 ◽  
Author(s):  
Terumitsu Azuma ◽  
Yuen Yung Hui ◽  
Oliver Y. Chen ◽  
Yuh-Lin Wang ◽  
Huan-Cheng Chang
Keyword(s):  
Laser Power ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Point Of Care Testing ◽  
Incident Laser Power ◽  
Latex Beads ◽  
Fold Reduction

Abstract Temperature sensing is a promising method of enhancing the detection sensitivity of lateral flow immunoassay for point-of-care testing. A temperature increase of more than 100 °C can be readily achieved by photoexcitation of reporters like gold nanoparticles (GNPs) or colored latex beads (CLBs) on the strips with a laser power below 100 mW. Despite its promise, processes involved in the photothermal detection have not yet been well-characterized. Here, we provide a fundamental understanding of this thermometric assay by combining experiments and simulations using non-fluorescent CLBs as the reporters deposited on nitrocellulose membrane. By measuring the dependence of temperature rises on the number density of membrane-bound CLBs, we determined a 1.5-fold enhancement of the light absorption at 520 nm by the beads (diameter of 0.4 μm). The enhancement, however, was compromised by a 5-fold reduction of the incident laser power due to multiple scattering of the light in this highly porous medium. The limit of detection was measured to be 1 × 105 particles/mm2. In line with previous studies using GNPs as the reporters, the CLB-based thermometric assay provides a 10× higher sensitivity than color visualization, as demonstrated with the immunoassay for nucleocapsid proteins of the SARS-CoV-2 virus.

Portable and multiplexed lateral flow immunoassay reader based on SERS for highly sensitive point-of-care testing

2020 ◽  
Vol 168 ◽  
pp. 112524 ◽  
Author(s):  
Rui Xiao ◽  
Luchun Lu ◽  
Zhen Rong ◽  
Chongwen Wang ◽  
Yongjin Peng ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Point Of Care Testing ◽  
Highly Sensitive

scholarly journals Point of care testing evaluation of lateral flow immunoassay for diagnosis of Cryptococcus Meningitis in Kenya, 2017

2019 ◽  
Author(s):  
Lawrence Kirimi Gitonga ◽  
Waqo Gufu Boru ◽  
Arthur Kwena ◽  
Marybeth Maritim ◽  
Joyce Wamicwe ◽  
...  
Keyword(s):  
Point Of Care ◽  
Cross Sectional Study ◽  
Latex Agglutination ◽  
Capillary Blood ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Point Of Care Testing ◽  
Cross Sectional ◽  
India Ink ◽  
Cryptococcus Meningitis

Abstract Objectives: The objective of this study was to evaluate the performance of lateral flow immunoassay (LFA) against latex agglutination (LA), India ink and culture in point-of-care diagnosis of Cryptococcus meningitis (CM). We conducted a cross-sectional study among patients with suspected CM at Mbagathi Hospital, Nairobi, April-July 2017. Results: Of 124 capillary blood and serum and 99 cerebrospinal fluid (CSF) samples, the agreement between LFA and LA on serum was 94.4%, kappa (0.88), sensitivity (100%) and specificity (91%). LFA and LA on CSF, was 97.9%, kappa (0.96), sensitivity (100%) and specificity (96%). LFA and India ink was 96.9%, kappa (0.94), sensitivity (100%) and specificity (94.1%). On CSF culture, the agreement was 72.7%, kappa (0.43), sensitivity (100%) and specificity (64%). The agreement of LFA on capillary blood, serum and CSF was 100% with kappa (1.00), sensitivity and specificity of 100%

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