scholarly journals Rapid Point-of-Care Testing for SARS-CoV-2 Virus Nucleic Acid Detection by an Isothermal and Nonenzymatic Signal Amplification System Coupled with a Lateral Flow Immunoassay Strip

2021 ◽  
pp. 129899
Author(s):  
Mingyuan Zou ◽  
Feiya Su ◽  
Rui Zhang ◽  
Xinglu Jiang ◽  
Han Xiao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Nucleic Acid Detection ◽  
Point Of Care Testing ◽  
Amplification System ◽  
Signal Amplification System

A signal amplification system on a lateral flow immunoassay detecting for hepatitis e‐antigen in human blood samples

2019 ◽  
Vol 91 (7) ◽  
pp. 1301-1306 ◽  
Author(s):  
Jinhong Si ◽  
Jinfeng Li ◽  
Ling Zhang ◽  
Weiyun Zhang ◽  
Jinxiu Yao ◽  
...  
Keyword(s):  
Human Blood ◽  
Signal Amplification ◽  
Hepatitis E ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Blood Samples ◽  
Amplification System ◽  
Human Blood Samples ◽  
Signal Amplification System

A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing

Lab on a Chip ◽  
2012 ◽  
Vol 12 (24) ◽  
pp. 5155 ◽  
Author(s):  
Yuriko Oyama ◽  
Toshihisa Osaki ◽  
Koki Kamiya ◽  
Ryuji Kawano ◽  
Tsutomu Honjoh ◽  
...  
Keyword(s):  
Glass Fiber ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Point Of Care Testing ◽  
Fiber Sheet

scholarly journals Quality Management for Point-Of-Care Testing of Pathogen Nucleic Acids: Chinese Expert Consensus

2021 ◽  
Vol 11 ◽  
Author(s):  
Xi Mo ◽  
Xueliang Wang ◽  
Zhaoqin Zhu ◽  
Yuetian Yu ◽  
Dong Chang ◽  
...  
Keyword(s):  
Public Health ◽  
Nucleic Acid ◽  
Quality Management ◽  
Clinical Laboratory ◽  
Point Of Care ◽  
Conventional Polymerase Chain Reaction ◽  
Laboratory Medicine ◽  
Expert Consensus ◽  
Nucleic Acid Detection ◽  
Point Of Care Testing

COVID-19 continues to circulate globally in 2021, while under the precise policy implementation of China’s public health system, the epidemic was quickly controlled, and society and the economy have recovered. During the pandemic response, nucleic acid detection of SARS-CoV-2 has played an indispensable role in the first line of defence. In the cases of emergency operations or patients presenting at fever clinics, nucleic acid detection is required to be performed and reported quickly. Therefore, nucleic acid point-of-care testing (POCT) technology for SARS-CoV-2 identification has emerged, and has been widely carried out at all levels of medical institutions. SARS-CoV-2 POCT has served as a complementary test to conventional polymerase chain reaction (PCR) batch tests, thus forming an experimental diagnosis platform that not only guarantees medical safety but also improves quality services. However, in view of the complexity of molecular diagnosis and the biosafety requirements involved, pathogen nucleic acid POCT is different from traditional blood-based physical and chemical index detection. No guidelines currently exist for POCT quality management, and there have been inconsistencies documented in practical operation. Therefore, Shanghai Society of Molecular Diagnostics, Shanghai Society of Laboratory Medicine, Clinical Microbiology Division of Shanghai Society of Microbiology and Shanghai Center for Clinical Laboratory have cooperated with experts in laboratory medicine to generate the present expert consensus. Based on the current spectrum of major infectious diseases in China, the whole-process operation management of pathogen POCT, including its application scenarios, biosafety management, personnel qualification, performance verification, quality control, and result reporting, are described here. This expert consensus will aid in promoting the rational application and robust development of this technology in public health defence and hospital infection management.

scholarly journals Point of care testing evaluation of lateral flow immunoassay for diagnosis of Cryptococcus Meningitis in Kenya, 2017

2019 ◽  
Author(s):  
Lawrence Kirimi Gitonga ◽  
Waqo Gufu Boru ◽  
Arthur Kwena ◽  
Marybeth Maritim ◽  
Joyce Wamicwe ◽  
...  
Keyword(s):  
Point Of Care ◽  
Cross Sectional Study ◽  
Latex Agglutination ◽  
Capillary Blood ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Point Of Care Testing ◽  
Cross Sectional ◽  
India Ink ◽  
Cryptococcus Meningitis

Abstract Objectives: The objective of this study was to evaluate the performance of lateral flow immunoassay (LFA) against latex agglutination (LA), India ink and culture in point-of-care diagnosis of CM. We conducted a cross-sectional study among patients with suspected CM at Mbagathi Hospital, Nairobi, April-July 2017. Results: Of 124 capillary blood and serum and 99 cerebrospinal fluid (CSF) samples, the agreement between LFA and LA on serum was 94.4%, kappa (0.88), sensitivity (100%) and specificity (91%). LFA and LA on CSF, was 97.9%, kappa (0.96), sensitivity (100%) and specificity (96%). LFA and India ink was 96.9%, kappa (0.94), sensitivity (100%) and specificity (94.1%). On CSF culture, the agreement was 72.7%, kappa (0.43), sensitivity (100%) and specificity (64%). The agreement of LFA on capillary blood, serum and CSF was 100% with kappa (1.00), sensitivity and specificity of 100%

scholarly journals The Application of Lateral Flow Immunoassay in Point of Care Testing: A Review

2016 ◽  
Vol 8 (3) ◽  
Author(s):  
Kan Wang ◽  
Weijian Qin ◽  
Yafei Hou ◽  
Kun Xiao ◽  
Wenqiang Yan
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Point Of Care Testing

scholarly journals Lower Urine Tenofovir Concentrations among Individuals Taking Tenofovir Alafenamide versus Tenofovir Disoproxil Fumarate: Implications for Point-of-Care Testing

2021 ◽  
Author(s):  
Kelly A Johnson ◽  
Xin Niu ◽  
David V Glidden ◽  
Jose R Castillo-Mancilla ◽  
Jenna Yager ◽  
...  
Keyword(s):  
Tenofovir Disoproxil Fumarate ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Directly Observed Therapy ◽  
Point Of Care Testing ◽  
Separate Point ◽  
Tenofovir Alafenamide ◽  
Adherence Monitoring ◽  
Therapy Studies

Abstract From directly-observed-therapy studies, urine tenofovir (TFV) levels were 74% lower when taking tenofovir alafenamide (TAF) versus tenofovir disoproxil fumarate. Urine TFV remains quantifiable across a range of TAF adherence patterns, but a separate point-of-care lateral flow immunoassay with a lower TFV threshold will be needed to support TAF adherence monitoring.

Rapid developments in lateral flow immunoassay for nucleic acid detection

The Analyst ◽  
2021 ◽  
Author(s):  
Chujun Zheng ◽  
Kan Wang ◽  
Wei Zheng ◽  
Yuemeng Cheng ◽  
Tangan Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Nucleic Acid Detection ◽  
Research Directions ◽  
Main Research ◽  
Lateral Flow Assays

Schematic of two main research directions of lateral flow assays for nucleic acid detection.

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