Infrared spectral histopathology for cancer diagnosis: a novel approach for automated pattern recognition of colon adenocarcinoma

The Analyst ◽  
2014 ◽  
Vol 139 (16) ◽  
pp. 4005-4015 ◽  
Author(s):  
Jayakrupakar Nallala ◽  
Marie-Danièle Diebold ◽  
Cyril Gobinet ◽  
Olivier Bouché ◽  
Ganesh Dhruvananda Sockalingum ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Pattern Recognition ◽  
Cancer Diagnosis ◽  
Colon Adenocarcinoma ◽  
Label Free ◽  
Tissue Arrays ◽  
Novel Approach ◽  
Infrared Spectral ◽  
Automated Pattern Recognition ◽  
Spectral Histopathology

Automated and label-free colon cancer diagnosis and identification of tumor-associated features using FTIR spectral histopathology directly on paraffinized tissue arrays.

Infrared spectral imaging as a novel approach for histopathological recognition in colon cancer diagnosis

2012 ◽  
Vol 17 (11) ◽  
pp. 116013 ◽  
Author(s):  
Jayakrupakar Nallala ◽  
Cyril Gobinet ◽  
Marie-Danièle Diebold ◽  
Valérie Untereiner ◽  
Olivier Bouché ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Diagnosis ◽  
Spectral Imaging ◽  
Novel Approach ◽  
Infrared Spectral

scholarly journals Label-free classification of colon cancer grading using infrared spectral histopathology

2016 ◽  
Vol 187 ◽  
pp. 105-118 ◽  
Author(s):  
C. Kuepper ◽  
F. Großerueschkamp ◽  
A. Kallenbach-Thieltges ◽  
A. Mosig ◽  
A. Tannapfel ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Label Free ◽  
Infrared Spectral ◽  
Poorly Differentiated ◽  
Well Differentiated ◽  
Free Classification ◽  
Cancer Grading ◽  
Spectral Histopathology ◽  
Good Agreement

In recent years spectral histopathology (SHP) has been established as a label-free method to identify cancer within tissue. Herein, this approach is extended. It is not only used to identify tumour tissue with a sensitivity of 94% and a specificity of 100%, but in addition the tumour grading is determined. Grading is a measure of how much the tumour cells differ from the healthy cells. The grading ranges from G1 (well-differentiated), to G2 (moderately differentiated), G3 (poorly differentiated) and in rare cases to G4 (anaplastic). The grading is prognostic and is needed for the therapeutic decision of the clinician. The presented results show good agreement between the annotation by SHP and by pathologists. A correlation matrix is presented, and the results show that SHP provides prognostic values in colon cancer, which are obtained in a label-free and automated manner. It might become an important automated diagnostic tool at the bedside in precision medicine.

Abstract 2741: Spectral histopathology of paraffinised colon tissue microarrays: a new approach by infrared spectral imaging for colon cancer diagnosis

2010 ◽  
Author(s):  
Jayakrupakar Nallala ◽  
Cyril Gobinet ◽  
Olivier Piot ◽  
Valérie Untereiner ◽  
Marie Danièle Diebold ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Diagnosis ◽  
Spectral Imaging ◽  
Tissue Microarrays ◽  
Colon Tissue ◽  
New Approach ◽  
Infrared Spectral ◽  
Spectral Histopathology

scholarly journals Label free technologies 3: infrared imaging applied to paraffinized tissue microarrays for colon cancer diagnosis

2013 ◽  
Vol 8 (S1) ◽  
Author(s):  
Ganesh D Sockalingum ◽  
Jayakrupakar Nallala ◽  
Marie-Danièle Diebold ◽  
Cyril Gobinet ◽  
Olivier Piot ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Diagnosis ◽  
Infrared Imaging ◽  
Tissue Microarrays ◽  
Label Free

Spectral histopathology of colon cancer tissue sections by Raman imaging with 532 nm excitation provides label free annotation of lymphocytes, erythrocytes and proliferating nuclei of cancer cells

The Analyst ◽  
2013 ◽  
Vol 138 (14) ◽  
pp. 4035 ◽  
Author(s):  
Laven Mavarani ◽  
Dennis Petersen ◽  
Samir F. El-Mashtoly ◽  
Axel Mosig ◽  
Andrea Tannapfel ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Raman Imaging ◽  
Cancer Tissue ◽  
Label Free ◽  
Colon Cancer Tissue ◽  
Tissue Sections ◽  
532 Nm ◽  
Spectral Histopathology

Immunohistochemistry, histopathology and infrared spectral histopathology of colon cancer tissue sections

2012 ◽  
Vol 6 (1) ◽  
pp. 88-100 ◽  
Author(s):  
Angela Kallenbach-Thieltges ◽  
Frederik Großerüschkamp ◽  
Axel Mosig ◽  
Max Diem ◽  
Andrea Tannapfel ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Tissue ◽  
Colon Cancer Tissue ◽  
Tissue Sections ◽  
Infrared Spectral ◽  
Spectral Histopathology

scholarly journals Tethered Lipid Membranes as a Nanoscale Arrangement Towards Non-Invasive Analysis of Acute Pancreatitis

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 755
Author(s):  
Rima Budvytyte ◽  
Akvile Milasiute ◽  
Dalius Vitkus ◽  
Kestutis Strupas ◽  
Aiste Gulla ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Lipid Membranes ◽  
Electrochemical Impedance ◽  
Membrane Integrity ◽  
Electrochemical Technique ◽  
Label Free ◽  
Non Invasive ◽  
Novel Approach ◽  
Analytical System ◽  
Shock Proteins

Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity.

scholarly journals Infrared spectral histopathology using haematoxylin and eosin (H&E) stained glass slides: a major step forward towards clinical translation

The Analyst ◽  
2017 ◽  
Vol 142 (8) ◽  
pp. 1258-1268 ◽  
Author(s):  
Michael J. Pilling ◽  
Alex Henderson ◽  
Jonathan H. Shanks ◽  
Michael D. Brown ◽  
Noel W. Clarke ◽  
...  
Keyword(s):  
Diagnostic Tool ◽  
Clinical Translation ◽  
Great Promise ◽  
Stained Glass ◽  
Major Step ◽  
Glass Slides ◽  
Important Diagnostic Tool ◽  
Infrared Spectral ◽  
Spectral Histopathology

Infrared spectral histopathology has shown great promise as an important diagnostic tool, with the potential to complement current pathological methods.

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