A highly sensitive and selective aptasensor based on fluorescence polarization for the rapid determination of oncoprotein vascular endothelial growth factor (VEGF)

2014 ◽  
Vol 6 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Sheng-E Wang ◽  
Yong Huang ◽  
Kun Hu ◽  
Jianniao Tian ◽  
Shulin Zhao
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Fluorescence Polarization ◽  
Rapid Determination ◽  
Vascular Endothelial ◽  
Highly Sensitive ◽  
Endothelial Growth Factor

A novel approach for the discrimination of culture medium from Vascular Endothelial Growth Factor (VEGF) overexpressing colorectal cancer cells

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sinem Tunçer ◽  
Rafig Gurbanov
Keyword(s):  
Colorectal Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Ftir Spectroscopy ◽  
Cancer Cells ◽  
Lower Amount ◽  
Vascular Endothelial ◽  
Colorectal Cancer Cells ◽  
Endothelial Growth Factor

AbstractObjectivesThe expression level of Vascular Endothelial Growth Factor (VEGF) is assumed as a prognostic marker for several tumor types, including colorectal cancer. Therefore, the determination of pre- and post-therapy levels of VEGF appears to have great value in the assessment of tumor prognosis. Enzyme-Linked Immunosorbent Assay (ELISA) is commonly used for the determination of serum or plasma VEGF levels, but the method is costly and time-consuming. In this study, we aimed to describe a rapid and cost-effective analysis method to discriminate VEGF overexpressing colorectal cancer-derived conditioned medium (CM).MethodsAttenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, combined with Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA), was used to differentiate VEGF overexpressing colorectal cancer cell line CM from CM obtained from the corresponding control cells which express and secrete relatively lower amount of VEGF.ResultsSamples belong to VEGF overexpressing colorectal cancer cells were clearly distinguished from the control group with very high PC scores as PC1 + PC2 = 96%. Besides, a 100% accurate distinction between these two groups was achieved by the LDA analysis.ConclusionsATR-FTIR spectroscopy combined with pattern recognition techniques was able to discriminate CM of VEGF overexpressing colorectal cancer cells with high efficiency and accuracy.

scholarly journals Determination of Vascular Endothelial Growth Factor (VEGF) and Tie-2 Levels in Patients with Primary Hypertension

2013 ◽  
Vol 62 (18) ◽  
pp. C93
Author(s):  
Ahmet Kemal Filiz ◽  
Hakan Güneş ◽  
Ercan Özdemir ◽  
Handan Güneş ◽  
Mehmet Birhan Yılmaz
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Primary Hypertension ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

scholarly journals A fluorescence polarization assay for identifying ligands that bind to vascular endothelial growth factor

2008 ◽  
Vol 378 (1) ◽  
pp. 8-14 ◽  
Author(s):  
Kimberly J. Peterson ◽  
Jack D. Sadowsky ◽  
Elizabeth A. Scheef ◽  
Soumen Pal ◽  
Katerina D. Kourentzi ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Fluorescence Polarization ◽  
Vascular Endothelial ◽  
Fluorescence Polarization Assay ◽  
Endothelial Growth Factor

scholarly journals The Determination of Appendicitis from Folate Acid and Vascular Endothelial Growth Factor Level in Animal Model: A Review

2021 ◽  
Vol 9 (F) ◽  
pp. 395-398
Author(s):  
Erjan Fikri ◽  
Putri Chairani Eyanoer
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Animal Model ◽  
Growth Factor ◽  
Healing Process ◽  
Vascular Endothelial ◽  
Tissue Healing ◽  
Study Results ◽  
Abdominal Emergencies ◽  
Endothelial Growth Factor

BACKGROUND: Appendicitis is one of the most common abdominal emergencies that require surgery in children. The morbidity and mortality rate in appendicitis is greatly affected by rupture or leakage of the appendix. In establishing the diagnosis of appendicitis, several modalities are acknowledged, namely, pediatric appendicitis score and ultrasound. Pathologically, severity and complications of appendicitis occur related to good vascularization and tissue healing process through process of angiogenesis influenced by folic acid (FA) and vascular endothelial growth factor (VEGF). This is clinically important as currently non-operative and non-invasive therapies were developing in stratification of mild appendicitis. AIM: This study aimed to review the determination of appendicitis from FA and VEGF levels in animal model. METHODS: This study was conducted in accordance with the PRISMA guidelines for reporting systematic reviews. Articles were reviewed for relation of FA and VEGF in determining appendicitis in PubMed and Science Direct. Articles on experimental animal model published from 1990 to 2020 were included, while articles in English were excluded from the study. RESULTS: The articles we reviewed conduct an assessment of appendicitis by FA and VEGF level in animal model. Three articles were reviewed ranging from 1990 to 2020. One article presented a significant association of decreased level of FA in determining appendicitis while the other two mentioned trends of decreased level of FA and VEGF in appendicitis without statistically significance. CONCLUSION: Reduction of folate acid levels could be a critical prescient factor for the weight of muddled an appendicitis in animal model, however, there was a pattern demonstrating low estimation of VEGF as an indicator of appendicitis and convoluted appendix with no measurable note worthiness appeared.

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