Time-resolved fluorescent detection of Hg2+ in a complex environment by conjugating magnetic nanoparticles with a triple-helix molecular switch

2013 ◽  
Vol 49 (61) ◽  
pp. 6915 ◽  
Author(s):  
Jing Zheng ◽  
Yuhong Nie ◽  
Yaping Hu ◽  
Jishan Li ◽  
Yinhui Li ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Triple Helix ◽  
Molecular Switch ◽  
Fluorescent Detection ◽  
Complex Environment ◽  
Time Resolved

scholarly journals Highly Sensitive Fluorescent Detection of Acetylcholine Based on the Enhanced Peroxidase-Like Activity of Histidine Coated Magnetic Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1207
Author(s):  
Hong Jae Cheon ◽  
Quynh Huong Nguyen ◽  
Moon Il Kim
Keyword(s):  
Magnetic Nanoparticles ◽  
High Sensitivity ◽  
High Specificity ◽  
Choline Oxidase ◽  
Fluorescent Detection ◽  
One Pot ◽  
Site Structure ◽  
Specificity And Sensitivity ◽  
Highly Sensitive ◽  
Peroxidase Mimics

Inspired by the active site structure of natural horseradish peroxidase having iron as a pivotal element with coordinated histidine residues, we have developed histidine coated magnetic nanoparticles (His@MNPs) with relatively uniform and small sizes (less than 10 nm) through one-pot heat treatment. In comparison to pristine MNPs and other amino acid coated MNPs, His@MNPs exhibited a considerably enhanced peroxidase-imitating activity, approaching 10-fold higher in catalytic reactions. With the high activity, His@MNPs then were exploited to detect the important neurotransmitter acetylcholine. By coupling choline oxidase and acetylcholine esterase with His@MNPs as peroxidase mimics, target choline and acetylcholine were successfully detected via fluorescent mode with high specificity and sensitivity with the limits of detection down to 200 and 100 nM, respectively. The diagnostic capability of the method is demonstrated by analyzing acetylcholine in human blood serum. This study thus demonstrates the potential of utilizing His@MNPs as peroxidase-mimicking nanozymes for detecting important biological and clinical targets with high sensitivity and reliability.

scholarly journals Magneto-Optical Characteristics of Streptavidin-Coated Fe3O4@Au Core-Shell Nanoparticles for Potential Applications on Biomedical Assays

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Chin-Wei Lin ◽  
Jian-Ming Chen ◽  
You-Jun Lin ◽  
Ling-Wei Chao ◽  
Sin-Yi Wei ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Faraday Effect ◽  
Au Nanoparticles ◽  
Optical Characteristics ◽  
Enzyme Linked Immunosorbent Assay ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Time Resolved ◽  
Biotechnology Research ◽  
Core Shell Nanoparticles

Abstract Recently, gold-coated magnetic nanoparticles have drawn the interest of researchers due to their unique magneto-plasmonic characteristics. Previous research has found that the magneto-optical Faraday effect of gold-coated magnetic nanoparticles can be effectively enhanced because of the surface plasmon resonance of the gold shell. Furthermore, gold-coated magnetic nanoparticles are ideal for biomedical applications because of their high stability and biocompatibility. In this work, we synthesized Fe3O4@Au core-shell nanoparticles and coated streptavidin (STA) on the surface. Streptavidin is a protein which can selectively bind to biotin with a strong affinity. STA is widely used in biotechnology research including enzyme-linked immunosorbent assay (ELISA), time-resolved immunofluorescence (TRFIA), biosensors, and targeted pharmaceuticals. The Faraday magneto-optical characteristics of the biofunctionalized Fe3O4@Au nanoparticles were measured and studied. We showed that the streptavidin-coated Fe3O4@Au nanoparticles still possessed the enhanced magneto-optical Faraday effect. As a result, the possibility of using biofunctionalized Fe3O4@Au nanoparticles for magneto-optical biomedical assays should be explored.

scholarly journals Time-resolved Fluorescent Detection for Glucose Using a Complex of Luminescent Layered Titanates and Enzymes

2017 ◽  
Vol 33 (9) ◽  
pp. 989-991 ◽  
Author(s):  
Yuki SAKAGUCHI ◽  
Tomoka MINAMIKAWA ◽  
Mayuko YAMAMURO ◽  
Tadayuki TSUJITA ◽  
Toshihisa UEDA ◽  
...  
Keyword(s):  
Fluorescent Detection ◽  
Time Resolved

A sensitive SERS assay for detecting proteins and nucleic acids using a triple-helix molecular switch for cascade signal amplification

2014 ◽  
Vol 50 (66) ◽  
pp. 9409-9412 ◽  
Author(s):  
Sujuan Ye ◽  
Yanying Wu ◽  
Wen Zhang ◽  
Na Li ◽  
Bo Tang
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Triple Helix ◽  
Detection System ◽  
Molecular Switch ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Multiple Cycle ◽  
Enhanced Raman Scattering

A sensitive surface-enhanced Raman scattering (SERS) detection system is developed for proteins and nucleic acids based on a triple-helix molecular switch for multiple cycle signal amplification.

Magnetic Nanoparticles to Recover Cellular Organelles and Study the Time Resolved Nanoparticle-Cell Interactome throughout Uptake

Small ◽  
2014 ◽  
Vol 10 (16) ◽  
pp. 3307-3315 ◽  
Author(s):  
Filippo Bertoli ◽  
Gemma-Louise Davies ◽  
Marco P. Monopoli ◽  
Micheal Moloney ◽  
Yurii K. Gun'ko ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Time Resolved ◽  
Cellular Organelles

Triple-helix molecular-switch-actuated exponential rolling circular amplification for ultrasensitive fluorescence detection of miRNAs

The Analyst ◽  
2019 ◽  
Vol 144 (17) ◽  
pp. 5245-5253 ◽  
Author(s):  
Yihan Zhao ◽  
Yu Wang ◽  
Su Liu ◽  
Chonglin Wang ◽  
Jiaxu Liang ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Triple Helix ◽  
High Efficiency ◽  
Molecular Switch ◽  
Ultrasensitive Detection ◽  
Fluorescent Biosensing

We have developed a rapid and high-efficiency fluorescent biosensing platform based on triple-helix molecular-switch (THMS)-actuated exponential rolling circular amplification (RCA) strategy for the ultrasensitive detection of miR-21.

A versatile signal-on electrochemical biosensor for Staphylococcus aureus based on triple-helix molecular switch

2021 ◽  
Vol 326 ◽  
pp. 128842
Author(s):  
Rongfeng Cai ◽  
Zhongwen Zhang ◽  
Haohan Chen ◽  
Yaping Tian ◽  
Nandi Zhou
Keyword(s):  
Staphylococcus Aureus ◽  
Triple Helix ◽  
Electrochemical Biosensor ◽  
Molecular Switch

A triple-helix molecular switch photoelectrochemical biosensor for ultrasensitive microRNA detection based on position-controllable CdS//CdTe signal enhancement and switching

2020 ◽  
Vol 56 (19) ◽  
pp. 2909-2912 ◽  
Author(s):  
Wenchao Geng ◽  
Ruiying Yang
Keyword(s):  
Triple Helix ◽  
Molecular Switch ◽  
Signal Enhancement ◽  
Microrna Detection ◽  
Photoelectrochemical Biosensor

A triple-helix molecular switch photoelectrochemical biosensor is developed for ultrasensitive microRNA detection based on position-controllable CdS//CdTe signal enhancement and switching.

A simple fluorescent strategy based on triple-helix molecular switch for sensitive detection of chloramphenicol

2020 ◽  
Vol 224 ◽  
pp. 117415 ◽  
Author(s):  
Chunyan Tu ◽  
Yuanyuan Dai ◽  
Ying Zhang ◽  
Weiping Wang ◽  
Liang Wu
Keyword(s):  
Triple Helix ◽  
Molecular Switch ◽  
Sensitive Detection

Phosphorescent porphyrin probes in biosensors and sensitive bioassays

2000 ◽  
Vol 28 (2) ◽  
pp. 74-77 ◽  
Author(s):  
D. B. Papkovsky ◽  
T. O'Riordan ◽  
A. Soini
Keyword(s):  
Oxygen Sensor ◽  
Spectral Characteristics ◽  
Screening Method ◽  
High Sensitivity ◽  
Water Soluble ◽  
Fluorescent Detection ◽  
Rapid Screening ◽  
Time Resolved ◽  
Phosphorescence Lifetime ◽  
Practical Applications

Platinum(II) and palladium(II) complexes of porphyrins and related tetrapyrrolic pigments emit strong phosphorescence at room temperatures, which is characterized by long lifetimes falling into the sub-millisecond range and long-wave spectral characteristics. These features make the dyes useful as probes for a number of bioanalytical applications, particularly those employing time-resolved fluorescent detection. They can provide high sensitivity and selectivity, together with rather simple instrumental set-up. A number of analytical systems are now under development that are based on the use of phosphorescent porphyrin probes. Experimental results are presented on the following systems: (i) fibre-optic phosphorescence lifetime-based oxygen sensor on the basis of hydrophobic platinum-porphyrins and development of advanced sensing materials and prototype instrumentation; (ii) practical applications of the optical oxygen sensor, including a sensitive immunosensor that employs glucose oxidase labels, a rapid screening method for cell viability in microtitre-plate format, non-destructive measurement of oxygen in packaged foods and reagentless biosensors for metabolites (glucose, lactate); and (iii) the use of water-soluble platinum- and palladium-porphyrins as labels for ultra-sensitive time-resolved phosphorescence immunoassays.

Sign in / Sign up

Export Citation Format

Share Document