Development and validation of a UPLC method for screening potentially counterfeit anti-hypertensive drugs using design of experiment

2014 ◽  
Vol 6 (13) ◽  
pp. 4610-4616 ◽  
Author(s):  
Chiguru Vishnuvardhan ◽  
R. Srinivas ◽  
N. Satheeshkumar
Keyword(s):  
Liquid Chromatography ◽  
Design Of Experiment ◽  
Ultra Performance Liquid Chromatography ◽  
Reversed Phase ◽  
Uv Detection ◽  
Counterfeit Medicines ◽  
Development And Validation

An isocratic reversed phase ultra performance liquid chromatography (RP-UPLC) method was developed for screening counterfeit medicines with UV detection at 210 nm.

scholarly journals ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF COMBINATION OF ANTI-ASTHMATIC DRUGS MONTELUKAST AND DOXOFYLLINE

2021 ◽  
Vol 11 (6) ◽  
pp. 86-91
Author(s):  
Sachin N. Kapase
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Method Development ◽  
Analytical Techniques ◽  
Ultra Performance Liquid Chromatography ◽  
Hplc Method ◽  
Uv Spectrophotometry ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Layer Chromatography

For qualitative and quantitative analysis, various analytical techniques are available such as Ultraviolet (UV) Spectrophotometry, High-performance liquid chromatography (HPLC), High-performance thin layer chromatography (HPTLC). As per literature survey, there are some UV, HPLC, Ultra-Performance Liquid Chromatography (UPLC) and HPTLC analytical methods are developed for Montelukast and Doxofylline individually and in a combination with other drugs too, since yet there are no significant stability studies indicating HPLC method reported for Montelukast and Doxofylline combinations. In the current study, the HPLC method is developed and validated for simultaneous quantitative estimations of Montelukast and Doxofylline. These present techniques are more efficient and sensitive as compared to other analytical techniques.

scholarly journals Development and Validation of a Multiresidue Method for the Determination of Neonicotinoid and Macrocyclic Lactone Pesticide Residues in Milk, Fruits, and Vegetables by Ultra-Performance Liquid Chromatography/MS/MS

2010 ◽  
Vol 93 (2) ◽  
pp. 389-399 ◽  
Author(s):  
Alaa Kamel ◽  
Yaorong Qian ◽  
Elizabeth Kolbe ◽  
Charles Stafford
Keyword(s):  
Liquid Chromatography ◽  
Macrocyclic Lactone ◽  
Fruits And Vegetables ◽  
Ultra Performance Liquid Chromatography ◽  
Multiresidue Method ◽  
Target Analytes ◽  
Neonicotinoid Pesticides ◽  
Development And Validation ◽  
Concentration Levels

Abstract A multiresidue method was developed and validated for the determination of 13 neonicotinoid pesticides and metabolites, and nine macrocyclic lactone pesticides and veterinary drugs using SPE and ultra-performance liquid chromatography/MS/MS. The method was validated in milk, orange, spinach, apple, plum, watermelon, green bean, zucchini, broccoli, strawberry, grape, and tomato by analyzing replicates of residue-free control samples fortified with a mixture of 22 target analytes at three concentration levels. The recoveries of the analytes from the fortified matrixes were mostly within 70120, except for some of the neonicotinoid metabolites. The LOD values varied by analyte and matrix and ranged between 0.0012 ng/g. The developed method was successful in combining two widely different classes of compounds into a single analysis.

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