Kinase Activity Assay Using Unspecific Substrate or Specific Synthetic Peptides

2021 ◽  
pp. 229-237
Author(s):  
Jiahui Wang ◽  
Xiaolin Yang ◽  
Lin Xi ◽  
Xu Na Wu
Keyword(s):  
Synthetic Peptides ◽  
Kinase Activity ◽  
Activity Assay ◽  
Kinase Activity Assay

A rapid, multiplexed kinase activity assay using 8-plex iTRAQ labeling, SPE, and MALDI-TOF/TOF MS

The Analyst ◽  
2020 ◽  
Vol 145 (3) ◽  
pp. 992-1000
Author(s):  
Yu-Ching Liu ◽  
Fuu-Jen Tsai ◽  
Chao-Jung Chen
Keyword(s):  
Kinase Activity ◽  
Kinase Assay ◽  
Activity Assay ◽  
Peptide Substrates ◽  
Itraq Labeling ◽  
Cell Lysates ◽  
Maldi Tof ◽  
Kinase Activity Assay ◽  
Tof Ms

Synthesized peptide substrates with iTRAQ labeling has been used for in vitro kinase assay using cell lysates and detected by MALDI TOF/TOF MS.

A robust and quantitative assay platform for multiplexed, high throughput screening of protein kinase inhibitors

2016 ◽  
Vol 52 (81) ◽  
pp. 12112-12115 ◽  
Author(s):  
Jieon Lee ◽  
Il-Soo Park ◽  
Ginam Park ◽  
Kyukwang Cho ◽  
Hee-Sung Park ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Protein Kinase ◽  
High Throughput ◽  
High Throughput Screening ◽  
Kinase Activity ◽  
Kinase Inhibitors ◽  
Protein Kinase Inhibitors ◽  
Protein Kinase Activity ◽  
Activity Assay ◽  
Kinase Activity Assay

We present a new platform for multiplexed protein kinase activity assay using TiO2decorated graphene oxide (GO), which is applicable to high throughput inhibitor screening.

Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS

2014 ◽  
Vol 50 (90) ◽  
pp. 13960-13962 ◽  
Author(s):  
Zhenzhen Deng ◽  
Mingliang Ye ◽  
Yangyang Bian ◽  
Zheyi Liu ◽  
Fangjie Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
Kinase Activity ◽  
Maldi Ms ◽  
Time Dependent ◽  
Activity Assay ◽  
Dimethyl Labeling ◽  
Labeling Approach ◽  
Kinase Activity Assay

A multiplex isotope dimethyl labeling approach allowed MALDI MS to monitor the time dependent consumption of substrates and generation of products in one spot.

scholarly journals Cell kinase activity assay based on surface enhanced Raman spectroscopy

2009 ◽  
Vol 73 (2) ◽  
pp. 226-230 ◽  
Author(s):  
Zhicao Yue ◽  
Fengfeng Zhuang ◽  
Rajar Kumar ◽  
Ieong Wong ◽  
Stephen B. Cronin ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Kinase Activity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Activity Assay ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Kinase Activity Assay

scholarly journals Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay

2009 ◽  
Vol 131 (30) ◽  
pp. 10340-10341 ◽  
Author(s):  
Jeong Hoon Lee ◽  
Benjamin D. Cosgrove ◽  
Douglas A. Lauffenburger ◽  
Jongyoon Han
Keyword(s):  
Kinase Activity ◽  
Activity Assay ◽  
Kinase Activity Assay
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