scholarly journals In vitro dosimetry of agglomerates

Nanoscale ◽  
2014 ◽  
Vol 6 (13) ◽  
pp. 7325-7331 ◽  
Author(s):  
V. Hirsch ◽  
C. Kinnear ◽  
L. Rodriguez-Lorenzo ◽  
C. A. Monnier ◽  
B. Rothen-Rutishauser ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Diffusion Coefficients ◽  
Uptake Kinetics ◽  
Small Particles ◽  
Au Nps ◽  
Kinetics Of ◽  
Lower Diffusion ◽  
Uptake Mechanisms ◽  
In Vitro Dosimetry

A well-controlled route towards biocompatible agglomerated Au-NPs is reported, which span the range from small particles with high diffusion coefficients to larger particles with lower diffusion coefficients. Difference in uptake kinetics of single NPs and agglomerates can be explained by particokinetics, without the need to consider size-mediated cellular uptake mechanisms.

Cellular Uptake Kinetics of Neutral and Charged Chemicals in in Vitro Assays Measured by Fluorescence Microscopy

2018 ◽  
Vol 31 (8) ◽  
pp. 646-657 ◽  
Author(s):  
Fabian C. Fischer ◽  
Cedric Abele ◽  
Steven T. J. Droge ◽  
Luise Henneberger ◽  
Maria König ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cellular Uptake ◽  
Uptake Kinetics ◽  
In Vitro Assays ◽  
Kinetics Of

Cellular uptake kinetics of bortezomib in relation to efficacy in myeloma cells and the influence of drug transporters

2014 ◽  
Vol 75 (2) ◽  
pp. 281-291 ◽  
Author(s):  
Jannick Clemens ◽  
Anja Seckinger ◽  
Dirk Hose ◽  
Dirk Theile ◽  
Magdalena Longo ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Drug Transporters ◽  
Uptake Kinetics ◽  
Myeloma Cells ◽  
Kinetics Of

scholarly journals Aging in vitro and D-glucose uptake kinetics of diploid human fibroblasts

1981 ◽  
Vol 106 (1) ◽  
pp. 99-108 ◽  
Author(s):  
T. Cremer ◽  
K. Werdan ◽  
A. F. G. Stevenson ◽  
K. Lehner ◽  
O. Messerschmidt
Keyword(s):  
Glucose Uptake ◽  
Human Fibroblasts ◽  
Uptake Kinetics ◽  
Kinetics Of

Cell-internalizing nucleic acid aptamers: challenges and cellular uptake mechanisms for applications as pharmacological agents

2021 ◽  
Author(s):  
Samira Husen Alamudi ◽  
Michiko Kimoto ◽  
Ichiro Hirao
Keyword(s):  
Nucleic Acid ◽  
Cellular Uptake ◽  
In Vitro Selection ◽  
Pharmacological Agents ◽  
Nucleic Acid Aptamers ◽  
Uptake Mechanisms ◽  
Targeted Therapeutic

Nucleic acid aptamers, also regarded as chemical antibodies, manifest potentials for targeted therapeutic and delivery agents since they possess unique advantages over antibodies. Generated by an iterative in vitro selection...

scholarly journals A mathematical investigation into the uptake kinetics of nanoparticles in vitro

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254208
Author(s):  
Hannah West ◽  
Fiona Roberts ◽  
Paul Sweeney ◽  
Simon Walker-Samuel ◽  
Joseph Leedale ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Differential Equations ◽  
Mixed Culture ◽  
Uptake Kinetics ◽  
Multiphase System ◽  
Chemotherapy Drugs ◽  
Culture Model ◽  
The Impact ◽  
Kinetics Of

Nanoparticles have the potential to increase the efficacy of anticancer drugs whilst reducing off-target side effects. However, there remain uncertainties regarding the cellular uptake kinetics of nanoparticles which could have implications for nanoparticle design and delivery. Polymersomes are nanoparticle candidates for cancer therapy which encapsulate chemotherapy drugs. Here we develop a mathematical model to simulate the uptake of polymersomes via endocytosis, a process by which polymersomes bind to the cell surface before becoming internalised by the cell where they then break down, releasing their contents which could include chemotherapy drugs. We focus on two in vitro configurations relevant to the testing and development of cancer therapies: a well-mixed culture model and a tumour spheroid setup. Our mathematical model of the well-mixed culture model comprises a set of coupled ordinary differential equations for the unbound and bound polymersomes and associated binding dynamics. Using a singular perturbation analysis we identify an optimal number of ligands on the polymersome surface which maximises internalised polymersomes and thus intracellular chemotherapy drug concentration. In our mathematical model of the spheroid, a multiphase system of partial differential equations is developed to describe the spatial and temporal distribution of bound and unbound polymersomes via advection and diffusion, alongside oxygen, tumour growth, cell proliferation and viability. Consistent with experimental observations, the model predicts the evolution of oxygen gradients leading to a necrotic core. We investigate the impact of two different internalisation functions on spheroid growth, a constant and a bond dependent function. It was found that the constant function yields faster uptake and therefore chemotherapy delivery. We also show how various parameters, such as spheroid permeability, lead to travelling wave or steady-state solutions.

The impact of emulsion droplet size on in vitro lipolysis rate and in vivo plasma uptake kinetics of triglycerides and vitamin D3 in rats

2021 ◽  
Author(s):  
Morten J. Dille ◽  
Tuna Baydin ◽  
Kåre A. Kristiansen ◽  
Kurt I. Draget
Keyword(s):  
Vitamin D ◽  
Droplet Size ◽  
Vitamin D3 ◽  
Uptake Kinetics ◽  
Emulsion Droplet ◽  
In Vitro Lipolysis ◽  
The Impact ◽  
Kinetics Of

Emulsions with smaller droplets are more rapidly lipolyzed in the intestine, resulting in increased uptake to plasma of triglycerides. However, the uptake of vitamin D3 from the same emulsions is not significantly affected by droplet size.

Sign in / Sign up

Export Citation Format

Share Document