Fluorescence enhancement detection of uric acid based on water-soluble 3-mercaptopropionic acid-capped core/shell ZnS:Cu/ZnS

RSC Advances ◽  
2014 ◽  
Vol 4 (48) ◽  
pp. 25183-25188 ◽  
Author(s):  
Meihua Wang ◽  
Weifen Niu ◽  
Xin Wu ◽  
Lixia Li ◽  
Jun Yang ◽  
...  
Keyword(s):  
Uric Acid ◽  
Fluorescence Enhancement ◽  
High Sensitivity ◽  
Water Soluble ◽  
Core Shell ◽  
Lower Toxicity ◽  
Real Samples ◽  
Mercaptopropionic Acid

A simple core/shell ZnS:Cu/ZnS QD based fluorescence enhancement system is constructed for detecting uric acid. The method with high sensitivity, selectivity and lower toxicity features was applied for determination of UA in real samples.

scholarly journals Synthesis of water soluble chitosan stabilized gold nanoparticles and determination of uric acid

2014 ◽  
Vol 5 (2) ◽  
pp. 025014 ◽  
Author(s):  
Thi Lanh Le ◽  
Quang Khieu Dinh ◽  
Thai Hoa Tran ◽  
Hai Phong Nguyen ◽  
Thi Le Hien Hoang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Uric Acid ◽  
Water Soluble

scholarly journals Enzymic determination of inorganic phosphates, organic phosphates and phosphate-liberating enzymes by use of nucleoside phosphorylase-xanthine oxidase (dehydrogenase)-coupled reactions

1985 ◽  
Vol 230 (1) ◽  
pp. 255-260 ◽  
Author(s):  
H de Groot ◽  
H de Groot ◽  
T Noll
Keyword(s):  
Uric Acid ◽  
Xanthine Oxidase ◽  
High Sensitivity ◽  
Inorganic Pyrophosphatase ◽  
Enzyme Assays ◽  
Tetrazolium Salt ◽  
Nucleoside Phosphorylase ◽  
Organic Phosphates ◽  
Inorganic Phosphates

Coupled enzyme assays are described for measuring inorganic phosphates, organic phosphates and phosphate-liberating enzymes in biological material. The assays all determine Pi by its reaction with inosine, catalysed by nucleoside phosphorylase; this yields ribose 1-phosphate and hypoxanthine. The hypoxanthine is oxidized to uric acid by xanthine oxidase, and may be measured either by the absorbance of the uric acid, or by the formazan formed when a tetrazolium salt is used as the oxidant. The coupled enzyme assays are characterized by high sensitivity, quantitative utilization of phosphates and stoichiometric formation of the measurable products, measurement at pH 6.0-8.5, determination of phosphates within a single analytical step, and continuous measurement of phosphohydrolase activity in a corresponding rate assay. Examples include determinations of substrates such as Pi, PPi and AMP, and of enzymes such as 5′-nucleotidase, inorganic pyrophosphatase and glucose-6-phosphatase. Directions for further examples are given.

Sensitive electrochemical determination of dopamine and uric acid using AuNPs(EDAS)–rGO nanocomposites

2016 ◽  
Vol 8 (22) ◽  
pp. 4379-4390 ◽  
Author(s):  
Victor Vinoth ◽  
Jerry J. Wu ◽  
Sambandam Anandan
Keyword(s):  
Uric Acid ◽  
High Sensitivity ◽  
Electrochemical Determination ◽  
Synthesis Process ◽  
Potential Candidate ◽  
Sonochemical Synthesis ◽  
One Step ◽  
Sensing Application ◽  
Electro Catalytic Activity

AuNPs(EDAS) – rGO nanocomposites were prepared by a facile one step sonochemical synthesis process. Here, EDAS acts as an interlinker for anchoring gold on rGO and it also acts both as a reducing agent and a stabilizing agent. The AuNPs(EDAS) – rGO nanocomposites show excellent electro-catalytic activity towards oxidation of DA and UA. The AuNPs(EDAS) – rGO nanocomposites exhibit low detection limits, high sensitivity, it could be a potential candidate for sensing application and in biosensor technology in the future.

scholarly journals 3-Mercaptopropionic Acid Capped Ga2Se3nanocrystal-CYP3A4 Biosensor for the Determination of 17-Alpha-Ethinyl Estradiol in Water

Nano Hybrids ◽  
2012 ◽  
Vol 1 ◽  
pp. 1-22 ◽  
Author(s):  
E. Nxusani ◽  
P.M. Ndangili ◽  
R.A. Olowu ◽  
A. N. Jijana ◽  
T. Waryo ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Ethinyl Estradiol ◽  
Genetically Engineered ◽  
Water Soluble ◽  
Cytochrome P450 3A4 ◽  
Gallium Selenide ◽  
Mercaptopropionic Acid ◽  
Endocrine Disrupting Compound ◽  
Endocrine Disrupting

Water soluble and biocompatible 3-mercaptopropionic acid capped gallium selenide nanocrystals, were synthesized from hydrated gallium (III) perchlorate and selenide ions. The 3-mercaptopropionic acid capped gallium selenide nanocrystals, was non-fluorescent but showed a sharp UV-vis absorption maximum at 250 nm.The synthesized nanoparticle was used to develop an electrochemical biosensor for the detection of 17-alpha-ethinyl estradiol, an estrogenic endocrine disrupting compound (e-EDC). The biosensor was fabricated by potentiostatic deposition of novel gallium selenide nanocrystals on a L-cystine modified gold electrode, followed by covalent coupling of genetically engineered cytochrome P450-3A4 (CYP3A4), a Heme containing enzyme. The biosensor gave an electrochemical response at about-220 mV. The results revealed that 3-Mecarptopropanoic acid capped Gallium Selenide nanocrystals can be used in conjunction with CYP3A4 as an electrode modifier for the detection of 17-alpha ethinyl estradiol. The 3-Mecarptopropanoic acid capped Gallium Selenide nanoparticles exhibited a semiconductor like behaviour.

Nanocomposite based on restacked crystallites of β-NiS and Ppy for the determination of theophylline and uric acid on screen-printed electrodes

2019 ◽  
Vol 43 (48) ◽  
pp. 19397-19407
Author(s):  
P. Muthukumaran ◽  
R. Ramya ◽  
P. Thivya ◽  
J. Wilson ◽  
G. Ravi
Keyword(s):  
Uric Acid ◽  
Electrocatalytic Activity ◽  
High Sensitivity ◽  
Screen Printed Electrodes ◽  
Screen Printed

We synthesized calcinated β-NiS with a highly crystalline porous nature and mixed it with Ppy to prepare a nanocomposite, which exhibited high electrocatalytic activity and this was then used to detect theophylline and uric acid with high sensitivity and stability.

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