Mesopattern of immobilised bone morphogenetic protein-2 created by microcontact printing and dip-pen nanolithography influence C2C12 cell fate

RSC Advances ◽  
2014 ◽  
Vol 4 (100) ◽  
pp. 56809-56815 ◽  
Author(s):  
S. Oberhansl ◽  
A. G. Castaño ◽  
A. Lagunas ◽  
E. Prats-Alfonso ◽  
M. Hirtz ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Bone Morphogenetic Protein ◽  
Cell Fate ◽  
Microcontact Printing ◽  
C2c12 Cell ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Dip Pen Nanolithography

Making meso matter: bone morphogenetic protein-2 (BMP-2) mesopattern created by dip-pen nanolithography and microcontact printing were applied to cell differentiation.

scholarly journals Combined Effect of Midazolam and Bone Morphogenetic Protein-2 for Differentiation Induction from C2C12 Myoblast Cells to Osteoblasts

Pharmaceutics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 218 ◽  
Author(s):  
Yukihiko Hidaka ◽  
Risako Chiba-Ohkuma ◽  
Takeo Karakida ◽  
Kazuo Onuma ◽  
Ryuji Yamamoto ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Crystal Engineering ◽  
Drug Repositioning ◽  
C2c12 Cell ◽  
C2c12 Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Marker Genes ◽  
C2c12 Myoblast ◽  
Gamma Aminobutyric Acid ◽  
Morphogenetic Protein

In drug repositioning research, a new concept in drug discovery and new therapeutic opportunities have been identified for existing drugs. Midazolam (MDZ) is an anesthetic inducer used for general anesthesia. Here, we demonstrate the combined effects of bone morphogenetic protein-2 (BMP-2) and MDZ on osteogenic differentiation. An immortalized mouse myoblast cell line (C2C12 cell) was cultured in the combination of BMP-2 and MDZ (BMP-2+MDZ). The differentiation and signal transduction of C2C12 cells into osteoblasts were investigated at biological, immunohistochemical, and genetic cell levels. Mineralized nodules formed in C2C12 cells were characterized at the crystal engineering level. BMP-2+MDZ treatment decreased the myotube cell formation of C2C12 cells, and enhanced alkaline phosphatase activity and expression levels of osteoblastic differentiation marker genes. The precipitated nodules consisted of randomly oriented hydroxyapatite nanorods and nanoparticles. BMP-2+MDZ treatment reduced the immunostaining for both α1 and γ2 subunits antigens on the gamma-aminobutyric acid type A (GABAA) receptor in C2C12 cells, but enhanced that for BMP signal transducers. Our investigation showed that BMP-2+MDZ has a strong ability to induce the differentiation of C2C12 cells into osteoblasts and has the potential for drug repositioning in bone regeneration.

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