A facile label-free colorimetric aptasensor for ricin based on the peroxidase-like activity of gold nanoparticles

A novel strategy for the fabrication of a colorimetric aptasensor using label free gold nanoparticles (AuNPs) is proposed. The aptasensor consists of adenosine (AD) aptamer, AD and AuNPs. The strategy has been employed for the assay of adenosine deaminase (ADA) activity.

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Hybridization chain reaction-based colorimetric aptasensor of adenosine 5′-triphosphate on unmodified gold nanoparticles and two label-free hairpin probes

Biosensors and Bioelectronics ◽

10.1016/j.bios.2016.10.043 ◽

2017 ◽

Vol 89 ◽

pp. 1006-1012 ◽

Cited By ~ 43

Author(s):

Zhuangqiang Gao ◽

Zhenli Qiu ◽

Minghua Lu ◽

Jian Shu ◽

Dianping Tang

Keyword(s):

Gold Nanoparticles ◽

Hybridization Chain Reaction ◽

Label Free ◽

Chain Reaction ◽

Hairpin Probes ◽

Colorimetric Aptasensor

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A label-free colorimetric aptasensor for simple, sensitive and selective detection of Pt (II) based on platinum (II)-oligonucleotide coordination induced gold nanoparticles aggregation

Biosensors and Bioelectronics ◽

10.1016/j.bios.2016.05.080 ◽

2016 ◽

Vol 85 ◽

pp. 771-776 ◽

Cited By ~ 19

Author(s):

Daoqing Fan ◽

Qingfeng Zhai ◽

Weijun Zhou ◽

Xiaoqing Zhu ◽

Erkang Wang ◽

...

Keyword(s):

Gold Nanoparticles ◽

Selective Detection ◽

Label Free ◽

Colorimetric Aptasensor

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Label-free colorimetric aptasensor for rapid detection of aflatoxin B1 by utilizing cationic perylene probe and localized surface plasmon resonance of gold nanoparticles

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2020.128356 ◽

2020 ◽

Vol 320 ◽

pp. 128356 ◽

Cited By ~ 1

Author(s):

Jamras Lerdsri ◽

Wisan Chananchana ◽

Jantima Upan ◽

Tharinee Sridara ◽

Jaroon Jakmunee

Keyword(s):

Gold Nanoparticles ◽

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Aflatoxin B1 ◽

Plasmon Resonance ◽

Rapid Detection ◽

Localized Surface Plasmon Resonance ◽

Localized Surface Plasmon ◽

Label Free ◽

Colorimetric Aptasensor

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Synthesis of a silymarin-gold nanoparticle conjugate and analysis of its liver-protecting activity

Current Pharmaceutical Biotechnology ◽

10.2174/1389201022666210101163734 ◽

2021 ◽

Vol 22 ◽

Author(s):

Sergey Staroverov ◽

Sergey Kozlov ◽

Alexander Fomin ◽

Konstantib Gabalov ◽

Alexey Volkov ◽

...

Keyword(s):

Gold Nanoparticles ◽

Liver Disease ◽

Carbon Tetrachloride ◽

Glutathione Depletion ◽

Laboratory Animals ◽

Experimental Hepatitis ◽

Acute Liver Disease ◽

The Mean ◽

Extent Of Damage ◽

First Time

Background: The liver disease problem prompts investigators to search for new methods of liver treatment. Introduction: Silymarin (Sil) protects the liver by reducing the concentration of free radicals and the extent of damage to the cell membranes. A particularly interesting method to increase the bioavailability of Sil is to use synthesized gold nanoparticles (AuNPs) as reagents. The study considered whether it was possible to use the silymarin-AuNP conjugate as a potential liver-protecting drug. Method: AuNPs were conjugated to Sil and examine the liver-protecting activity of the conjugate. Experimental hepatitis and hepatocyte cytolysis after carbon tetrachloride actionwere used as a model system, and the experiments were conducted on laboratory animals. Result: For the first time, silymarin was conjugated to colloidal gold nanoparticles (AuNPs). Electron microscopy showed that the resultant preparations were monodisperse and that the mean conjugate diameter was 18–30 nm ± 0.5 nm (mean diameter of the native nanoparticles, 15 ± 0.5 nm). In experimental hepatitis in mice, conjugate administration interfered with glutathione depletion in hepatocytes in response to carbon tetrachloride was conducive to an increase in energy metabolism, and stimulated the monocyte–macrophage function of the liver. The results were confirmed by the high respiratory activity of the hepatocytes in cell culture. Conclusion: We conclude that the silymarin-AuNP conjugate holds promise as a liver-protecting agent in acute liver disease caused by carbon tetrachloride poisoning.

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Phosphatidylserine-Gold Nanoparticles (PS-AuNP) Induce Prostate and Breast Cancer Cell Apoptosis

Pharmaceutics ◽

10.3390/pharmaceutics13071094 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1094

Author(s):

Allan Radaic ◽

Nam E. Joo ◽

Soo-Hwan Jeong ◽

Seong-II Yoo ◽

Nicholas Kotov ◽

...

Keyword(s):

Breast Cancer ◽

Gold Nanoparticles ◽

Biomedical Applications ◽

Therapeutic Potential ◽

Control Treatment ◽

Track Record ◽

Males And Females ◽

Breast And Prostate Cancer ◽

First Time

Prostate and breast cancer are the current leading causes of new cancer cases in males and females, respectively. Phosphatidylserine (PS) is an essential lipid that mediates macrophage efferocytosis and is dysregulated in tumors. Therefore, developing therapies that selectively restore PS may be a potential therapeutic approach for carcinogenesis. Among the nanomedicine strategies for delivering PS, biocompatible gold nanoparticles (AuNPs) have an extensive track record in biomedical applications. In this study, we synthesized biomimetic phosphatidylserine-caped gold nanoparticles (PS-AuNPs) and tested their anticancer potential in breast and prostate cancer cells in vitro. We found that both cell lines exhibited changes in cell morphology indicative of apoptosis. After evaluating for histone-associated DNA fragments, a hallmark of apoptosis, we found significant increases in DNA fragmentation upon PS-AuNP treatment compared to the control treatment. These findings demonstrate the use of phosphatidylserine coupled with gold nanoparticles as a potential treatment for prostate and breast cancer. To the best of our knowledge, this is the first time that a phosphatidylserine-capped AuNP has been examined for its therapeutic potential in cancer therapy.

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Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

Scientific Reports ◽

10.1038/s41598-021-94380-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Rozita Abolhasan ◽

Balal Khalilzadeh ◽

Hadi Yousefi ◽

Sahar Samemaleki ◽

Forough Chakari-Khiavi ◽

...

Keyword(s):

Gold Nanoparticles ◽

Flexible Structures ◽

Modified Electrodes ◽

Electrochemical Immunosensor ◽

Lymphocytic Leukemia ◽

Orphan Receptor ◽

Detection Sensitivity ◽

Label Free ◽

Serum Samples ◽

Limit Of Quantification

AbstractIn the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01–1 pg mL−1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL−1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.

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