Synthesis of a silymarin-gold nanoparticle conjugate and analysis of its liver-protecting activity

2021 ◽  
Vol 22 ◽  
Author(s):  
Sergey Staroverov ◽  
Sergey Kozlov ◽  
Alexander Fomin ◽  
Konstantib Gabalov ◽  
Alexey Volkov ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Liver Disease ◽  
Carbon Tetrachloride ◽  
Glutathione Depletion ◽  
Laboratory Animals ◽  
Experimental Hepatitis ◽  
Acute Liver Disease ◽  
The Mean ◽  
Extent Of Damage ◽  
First Time

Background: The liver disease problem prompts investigators to search for new methods of liver treatment. Introduction: Silymarin (Sil) protects the liver by reducing the concentration of free radicals and the extent of damage to the cell membranes. A particularly interesting method to increase the bioavailability of Sil is to use synthesized gold nanoparticles (AuNPs) as reagents. The study considered whether it was possible to use the silymarin-AuNP conjugate as a potential liver-protecting drug. Method: AuNPs were conjugated to Sil and examine the liver-protecting activity of the conjugate. Experimental hepatitis and hepatocyte cytolysis after carbon tetrachloride actionwere used as a model system, and the experiments were conducted on laboratory animals. Result: For the first time, silymarin was conjugated to colloidal gold nanoparticles (AuNPs). Electron microscopy showed that the resultant preparations were monodisperse and that the mean conjugate diameter was 18–30 nm ± 0.5 nm (mean diameter of the native nanoparticles, 15 ± 0.5 nm). In experimental hepatitis in mice, conjugate administration interfered with glutathione depletion in hepatocytes in response to carbon tetrachloride was conducive to an increase in energy metabolism, and stimulated the monocyte–macrophage function of the liver. The results were confirmed by the high respiratory activity of the hepatocytes in cell culture. Conclusion: We conclude that the silymarin-AuNP conjugate holds promise as a liver-protecting agent in acute liver disease caused by carbon tetrachloride poisoning.

A Somatomedin Assay using Normal Rabbit Cartilage in Clinical Studies

1975 ◽  
Vol 53 (3) ◽  
pp. 403-409 ◽  
Author(s):  
R. Marvin Bala ◽  
Catherine Hankins ◽  
Glen R. Smith
Keyword(s):  
Liver Disease ◽  
Costal Cartilage ◽  
Elevated Serum ◽  
Biological Growth ◽  
Acute Liver Disease ◽  
Rabbit Cartilage ◽  
The Mean ◽  
Relationship Of ◽  
The Relationship ◽  
Low Serum

Somatomedin (SM) is a growth hormone (GH) dependent biological growth factor in serum. A SM assay, using mechanically sliced discs from young normal rabbit costal cartilage was developed and evaluated. This assay is as sensitive and precise, while much less laborious and costly, than the hypophysectomized rat cartilage SM assay. The elevated serum SM levels in patients with acromegaly returned to normal in two patients who were cured, and remained elevated in four incompletely treated patients. The low serum SM levels in five GH deficient patients were increased to normal after treatment with human GH. SM levels were normal in 10 patients with normal GH levels and short stature due to various causes. SM levels in serum were very low in patients with chronic renal insufficiency; the mean SM level increased significantly after renal transplantation, however, it increased only minimally after hemodialysis. SM levels were subnormal in 14 patients with severe chronic liver disease. One patient with severe acute liver disease had low serum SM levels which increased after clinical recovery. These results further suggest that the liver produces SM, while the relationship of the kidney and SM is not clear.

Vitamin D levels are not associated with non-alcoholic fatty liver disease severity in a Brazilian population

2020 ◽  
pp. 1-8
Author(s):  
Jeniffer Danielle M. Dutra ◽  
Quelson Coelho Lisboa ◽  
Silvia Marinho Ferolla ◽  
Carolina Martinelli M. L. Carvalho ◽  
Camila Costa M. Mendes ◽  
...  
Keyword(s):  
Vitamin D ◽  
Liver Disease ◽  
Fatty Liver ◽  
Disease Severity ◽  
Fatty Liver Disease ◽  
Hypovitaminosis D ◽  
Alcoholic Fatty Liver ◽  
Vitamin D Levels ◽  
The Mean ◽  
Alcoholic Fatty Liver Disease

Abstract. Some epidemiological evidence suggests an inverse correlation between non-alcoholic fatty liver disease (NAFLD) frequency and vitamin D levels. Likewise, a beneficial effect of vitamin D on diabetes mellitus (DM) and insulin resistance has been observed, but this is an unsolved issue. Thus, we aimed to investigate the prevalence of hypovitaminosis D in a NAFLD Brazilian population and its association with disease severity and presence of comorbidities. In a cross-sectional study, the clinical, biochemical and histological parameters of 139 NAFLD patients were evaluated according to two different cut-off points of serum 25-hydroxyvitamin D levels (20 ng/mL and 30 ng/mL). The mean age of the population was 56 ± 16 years, most patients were female (83%), 72% had hypertension, 88% dyslipidemia, 46% DM, 98% central obesity, and 82% metabolic syndrome. Serum vitamin D levels were < 30 ng/mL in 78% of the patients, and < 20 ng/mL in 35%. The mean vitamin D level was 24.3 ± 6.8 ng/mL. The comparison between the clinical, biochemical and histological characteristics of the patients according to the levels of vitamin D showed no significant difference. Most patients with NAFLD had hypovitaminosis D, but low vitamin D levels were not related to disease severity and the presence of comorbidities.

RESULTS OF INTRALESIONAL TRIAMCINOLONE ACETONIDE FOR CHALAZION TREATMENT

2011 ◽  
pp. 100-104
Author(s):  
Thi Thu Nguyen ◽  
Viet Hien Vo ◽  
Thi Em Do
Keyword(s):  
Success Rate ◽  
Key Words ◽  
Triamcinolone Acetonide ◽  
Lower Eyelid ◽  
Upper Eyelid ◽  
Interventional Trial ◽  
The Mean ◽  
First Time

The study use intralesional triamcinolone acetonide injection proceduce for chalazion treatment.1. Objectives: To evaluate results of intralesional triamcinolone acetonide injection for chalazion treatment. 2. Method: This noncomparative prospective interventional trial included 72 chalazions of 61 patients. 3. Results: 61 patients (72 chalazions) with 19 males (31.1%) và 42 females (68.9%), the mean age was 24 ± 9,78 years. 31.1% patients was the first time chalazion and 68.9% patients was more than one times chalazion including 78.6% patients was recurrent at the first position and 21.4% patients occur at new position. 72 chalazions with 16 (22.2%) chalazions was treated before and 56 (77.8%) chalazions wasn’t done that. 72 chalazions with 49 chalazions (68.1%) are local in upper eyelid and 23 chalazions (31.9%) are local in lower eyelid. The mean of chalazion diameter is 6.99 ± 3.03mm. Intralesional triamcinolone acetonide is injected to treat 72 chalazions with 16 (22.2%) chalazions are injected through the route of skin and 56 (77.8%) chalazions are injected through the route of conjunctiva. After 2 weeks follow-up, the success rate was 93.1% and 6.9% failed. 4. Conclusion: intralesional triamcinolone acetonide injection for chalazion treatment is really effective. Key words: chalazion, intralesional triamcinolone acetonide.

Association between the frequency of daily tooth brushing and development of nonalcoholic fatty liver disease

2021 ◽  
Author(s):  
Kazuki Yamamoto ◽  
Takashi Ikeya ◽  
Shuhei Okuyama ◽  
Katsuyuki Fukuda ◽  
Daiki Kobayashi
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Fatty Liver Disease ◽  
Primary Outcome ◽  
Tooth Brushing ◽  
Health Checkup ◽  
Abdominal Ultrasonography ◽  
Nonalcoholic Fatty Liver ◽  
The Mean

Background & Aims: This study aimed to evaluate the association between the frequency of daily tooth brushing and the development of nonalcoholic fatty liver disease (NAFLD). Methods: A retrospective longitudinal study was conducted from 2005 to 2012 at the Center for Preventive Medicine at St. Luke's International Hospital, Japan. Data on all participants who underwent a health checkup during the study period were collected. NAFLD was diagnosed by abdominal ultrasonography, and all participants who were diagnosed with NALFD at the time of their initial visit, consumed alcohol in any amount, or had received only one health checkup were excluded. The questionnaire for the frequency of daily tooth brushing was conducted as part of health checkups. The primary outcome was the risk of developing NAFLD according to the frequency of daily tooth brushing (1-2 times a day, or 3 times a day) compared to those who brush teeth once or less than once a day. Results: Data were collected from 25,804 people. A total of 3,289 (12.7%) participants developed NAFLD. The mean age was 45.2 years, and 6,901 (26.7%) of the participants were male. The risk of developing NAFLD significantly decreased with increased frequency of daily tooth brushing. Adjusted odds ratios (ORs) are as follows: Brushing teeth 1-2 times a day (OR: 0.85, 95% CI: 0.77-0.95), and 3 times a day (OR: 0.74, 95% CI: 0.67-0.82). Conclusions: Frequent tooth brushing was shown to significantly reduce the risk of developing NAFLD.　

scholarly journals Phosphatidylserine-Gold Nanoparticles (PS-AuNP) Induce Prostate and Breast Cancer Cell Apoptosis

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1094
Author(s):  
Allan Radaic ◽  
Nam E. Joo ◽  
Soo-Hwan Jeong ◽  
Seong-II Yoo ◽  
Nicholas Kotov ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Biomedical Applications ◽  
Therapeutic Potential ◽  
Control Treatment ◽  
Track Record ◽  
Males And Females ◽  
Breast And Prostate Cancer ◽  
First Time

Prostate and breast cancer are the current leading causes of new cancer cases in males and females, respectively. Phosphatidylserine (PS) is an essential lipid that mediates macrophage efferocytosis and is dysregulated in tumors. Therefore, developing therapies that selectively restore PS may be a potential therapeutic approach for carcinogenesis. Among the nanomedicine strategies for delivering PS, biocompatible gold nanoparticles (AuNPs) have an extensive track record in biomedical applications. In this study, we synthesized biomimetic phosphatidylserine-caped gold nanoparticles (PS-AuNPs) and tested their anticancer potential in breast and prostate cancer cells in vitro. We found that both cell lines exhibited changes in cell morphology indicative of apoptosis. After evaluating for histone-associated DNA fragments, a hallmark of apoptosis, we found significant increases in DNA fragmentation upon PS-AuNP treatment compared to the control treatment. These findings demonstrate the use of phosphatidylserine coupled with gold nanoparticles as a potential treatment for prostate and breast cancer. To the best of our knowledge, this is the first time that a phosphatidylserine-capped AuNP has been examined for its therapeutic potential in cancer therapy.

scholarly journals Neutrosophic ratio-type estimators for estimating the population mean

2021 ◽  
Author(s):  
Zaigham Tahir ◽  
Hina Khan ◽  
Muhammad Aslam ◽  
Javid Shabbir ◽  
Yasar Mahmood ◽  
...  
Keyword(s):  
Minimum Mean Square Error ◽  
Auxiliary Information ◽  
Population Parameter ◽  
Uncertain Information ◽  
Population Mean ◽  
Classical Statistics ◽  
Neutrosophic Statistics ◽  
The Mean ◽  
Type Data ◽  
First Time

AbstractAll researches, under classical statistics, are based on determinate, crisp data to estimate the mean of the population when auxiliary information is available. Such estimates often are biased. The goal is to find the best estimates for the unknown value of the population mean with minimum mean square error (MSE). The neutrosophic statistics, generalization of classical statistics tackles vague, indeterminate, uncertain information. Thus, for the first time under neutrosophic statistics, to overcome the issues of estimation of the population mean of neutrosophic data, we have developed the neutrosophic ratio-type estimators for estimating the mean of the finite population utilizing auxiliary information. The neutrosophic observation is of the form $${Z}_{N}={Z}_{L}+{Z}_{U}{I}_{N}\, {\rm where}\, {I}_{N}\in \left[{I}_{L}, {I}_{U}\right], {Z}_{N}\in [{Z}_{l}, {Z}_{u}]$$ Z N = Z L + Z U I N where I N ∈ I L , I U , Z N ∈ [ Z l , Z u ] . The proposed estimators are very helpful to compute results when dealing with ambiguous, vague, and neutrosophic-type data. The results of these estimators are not single-valued but provide an interval form in which our population parameter may have more chance to lie. It increases the efficiency of the estimators, since we have an estimated interval that contains the unknown value of the population mean provided a minimum MSE. The efficiency of the proposed neutrosophic ratio-type estimators is also discussed using neutrosophic data of temperature and also by using simulation. A comparison is also conducted to illustrate the usefulness of Neutrosophic Ratio-type estimators over the classical estimators.

scholarly journals Facile Green, Room-Temperature Synthesis of Gold Nanoparticles Using Combretum erythrophyllum Leaf Extract: Antibacterial and Cell Viability Studies against Normal and Cancerous Cells

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 893
Author(s):  
Olufunto T. Fanoro ◽  
Sundararajan Parani ◽  
Rodney Maluleke ◽  
Thabang C. Lebepe ◽  
Jose R. Varghese ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Viability ◽  
Leaf Extract ◽  
Room Temperature ◽  
Klebsiella Oxytoca ◽  
Cost Effective ◽  
Average Diameter ◽  
Antibacterial Activities ◽  
Inhibition Concentration ◽  
First Time

We herein report a facile, green, cost-effective, plant-mediated synthesis of gold nanoparticles (AuNPs) for the first time using Combretum erythrophyllum (CE) plant leaves. The synthesis was conducted at room temperature using CE leaf extract serving as a reducing and capping agent. The as-synthesized AuNPs were found to be crystalline, well dispersed, and spherical in shape with an average diameter of 13.20 nm and an excellent stability of over 60 days. The AuNPs showed broad-spectrum antibacterial activities against both pathogenic Gram-positive (Staphylococcus epidermidis (ATCC14990), Staphylococcus aureus (ATCC 25923), Mycobacterium smegmatis (MC 215)) and Gram-negative bacteria (Proteus mirabilis (ATCC 7002), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 13822), Klebsiella oxytoca (ATCC 8724)), with a minimum inhibition concentration of 62.5 µg/mL. In addition, the as-synthesized AuNPs were highly stable with exceptional cell viability towards normal cells (BHK- 21) and cancerous cancer cell lines (cervical and lung cancer).

scholarly journals P1398 Echocardiographic continuous monitoring of exercise stress test using probe fixation device

2020 ◽  
Vol 21 (Supplement_1) ◽  
Author(s):  
K Wdowiak-Okrojek ◽  
P Wejner-Mik ◽  
Z Bednarkiewicz ◽  
P Lipiec ◽  
J D Kasprzak
Keyword(s):  
Image Quality ◽  
Physical Exercise ◽  
Stress Test ◽  
Exercise Stress Test ◽  
Bicycle Ergometer ◽  
Exercise Stress ◽  
Fixation Device ◽  
The Mean ◽  
First Time

Abstract Background Stress echocardiography (SE) plays an important role among methods of noninvasive diagnosis of ischemic disease. Despite the advantages of physical exercise as the most physiologic stressor, it is difficult (bicycle ergometer) or impossible (treadmill) to obtain and maintain the acoustic window during the exercise. Recently, an innovative probe fixation device was introduced and a research plan was developed to assess the feasibility of external probe fixation during exercise echocardiography on a supine bicycle and upright treadmill exercise for the first time. Methods 37 subjects (36 men, mean age 39 ± 16 years, 21 healthy volunteers, 16 patients with suspected coronary artery disease) were included in this study. This preliminary testing stage included mostly men due to more problematic probe fixation in women. All subjects underwent a submaximal exercise stress test on a treadmill (17/37) or bicycle ergometer (11/37). Both sector and matrix probes were used. We assessed semi-quantitatively the quality of acquired apical views at each stage – the four-point grading system was used (0-no view, 1-suboptimal quality, 2-optimal quality, 3-very good quality), 2-3 sufficient for diagnosis. Results The mean time required for careful positioning of the probe and image optimization was 12 ± 3 min and shortened from 13,7 to 11,1 minutes (mean) in first vs second half of the cohort documenting learning curve. At baseline, 9 patients had at least one apical view of quality precluding reliable analysis. Those patients were excluded from further assessment. During stress, 17 patients maintained the optimal or very good quality of all apical views, whereas in 11 patients the quality significantly decreased during the stress test and required probe repositioning. The mean image quality score at baseline was 2,61 ± 0,48 and 2,25 ± 0,6 after exercise. Expectedly, good image quality was easier to obtain and maintain in the supine position (score 2,74 ± 0,44) points as compared with upright position (score 2,25 ± 0,57). Conclusion This preliminary, unique experience with external probe fixation device indicates that continuous acquisition and monitoring of echocardiographic images is feasible during physical exercise, and for the first time ever - also on the treadmill. This feasibility data stem from almost exclusively male patients and the estimated rate of sufficient image quality throughout the entire test is currently around 60%. We are hoping, that gaining more experience with the product could increase the success rate on exercise tests. Abstract P1398 Figure. Treadmill and ergometer stress test

scholarly journals Cellular Senescence in Liver Disease and Regeneration

2021 ◽  
Author(s):  
Sofia Ferreira-Gonzalez ◽  
Daniel Rodrigo-Torres ◽  
Victoria L. Gadd ◽  
Stuart J. Forbes
Keyword(s):  
Cell Cycle ◽  
Liver Disease ◽  
Cell Cycle Arrest ◽  
Genomic Instability ◽  
Cellular Senescence ◽  
Cell Populations ◽  
Cycle Arrest ◽  
Relevant Role ◽  
Extent Of Damage

AbstractCellular senescence is an irreversible cell cycle arrest implemented by the cell as a result of stressful insults. Characterized by phenotypic alterations, including secretome changes and genomic instability, senescence is capable of exerting both detrimental and beneficial processes. Accumulating evidence has shown that cellular senescence plays a relevant role in the occurrence and development of liver disease, as a mechanism to contain damage and promote regeneration, but also characterizing the onset and correlating with the extent of damage. The evidence of senescent mechanisms acting on the cell populations of the liver will be described including the role of markers to detect cellular senescence. Overall, this review intends to summarize the role of senescence in liver homeostasis, injury, disease, and regeneration.

THE INFLUENCE OF WEIGHT AND HEIGHT ON WEIGHT CHANGES ASSOCIATED WITH PREGNANCY IN WOMEN

1957 ◽  
Vol 15 (4) ◽  
pp. 423-429 ◽  
Author(s):  
THOMAS McKEOWN ◽  
R. G. RECORD
Keyword(s):  
Weight Change ◽  
Weight Changes ◽  
Body Build ◽  
Weight Increment ◽  
Time Table ◽  
The Mean ◽  
Significant Change ◽  
First Time

SUMMARY Three hundred and eighty-three women whose weights were recorded during and after pregnancy were divided into groups of short (under 64 in.) and tall (64 in. and over) individuals, and further subdivided according to an index of body build (based on the observed regression of antenatal weight on height). Differences between the weight 12 months after delivery and the antenatal weight (adjusted according to the number of days by which it preceded or followed the 124th day of gestation) were examined. The proportion of short women who added weight in this interval was relatively high for those who were thinnest at the antenatal examination, was lowest for those who were moderately thin and thereafter increased with increasing degree of obesity (from about 1 in 3 to 4 in 5); the proportion of tall women who added weight showed no significant change (Fig. 2). This variation in weight change according to height is exhibited by women pregnant for the first time (Table 3). For both short and tall women, the mean weight increment of those who added weight, and the mean weight decrement of those who lost weight, increased with increasing degree of obesity (Fig. 3). When weight change is expressed as a proportion of the antenatal weight these trends disappear (Fig. 4).

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