Polycations with excellent gene transfection ability based on PVP-g-PDMAEMA with random coil and micelle structures as non-viral gene vectors

2015 ◽  
Vol 3 (5) ◽  
pp. 911-918 ◽  
Author(s):  
Yuhua Song ◽  
Tingbin Zhang ◽  
Xiaoyan Song ◽  
Ling Zhang ◽  
Chunqiu Zhang ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Random Coil ◽  
Viral Gene ◽  
Core Shell ◽  
Random Coils ◽  
Gene Vectors ◽  
Spherical Core ◽  
Self Assembled

PVP-g-PDMAEMA formed random coils in water and PVP-g-PDMAEMA-b-PMMA self-assembled into spherical core–shell micelles. Both displayed excellent pDNA compacting abilities at an extremely low N/P ratio, with PVP-g-PDMAEMA-b-PMMA also showing excellent gear transfection efficiency.

scholarly journals Transient stealth coating of liver sinusoidal wall by anchoring two-armed PEG for retargeting nanomedicines

2020 ◽  
Vol 6 (26) ◽  
pp. eabb8133 ◽  
Author(s):  
Anjaneyulu Dirisala ◽  
Satoshi Uchida ◽  
Kazuko Toh ◽  
Junjie Li ◽  
Shigehito Osawa ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Critical Issue ◽  
Viral Gene ◽  
Liver Sinusoids ◽  
Gene Vectors ◽  
Sinusoidal Endothelium ◽  
Poly Ethylene ◽  
Peg Coating

A major critical issue in systemically administered nanomedicines is nonspecific clearance by the liver sinusoidal endothelium, causing a substantial decrease in the delivery efficiency of nanomedicines into the target tissues. Here, we addressed this issue by in situ stealth coating of liver sinusoids using linear or two-armed poly(ethylene glycol) (PEG)–conjugated oligo(l-lysine) (OligoLys). PEG-OligoLys selectively attached to liver sinusoids for PEG coating, leaving the endothelium of other tissues uncoated and, thus, accessible to the nanomedicines. Furthermore, OligoLys having a two-armed PEG configuration was ultimately cleared from sinusoidal walls to the bile, while OligoLys with linear PEG persisted in the sinusoidal walls, possibly causing prolonged disturbance of liver physiological functions. Such transient and selective stealth coating of liver sinusoids by two-arm-PEG-OligoLys was effective in preventing the sinusoidal clearance of nonviral and viral gene vectors, representatives of synthetic and nature-derived nanomedicines, respectively, thereby boosting their gene transfection efficiency in the target tissues.

Zn-promoted gene transfection efficiency for non-viral vectors: a mechanism study

2021 ◽  
Author(s):  
Xiao-Qi Yu ◽  
Rui-Mo Zhao ◽  
Yu Guo ◽  
Hui-Zhen Yang ◽  
Ji Zhang
Keyword(s):  
Gene Therapy ◽  
Nucleic Acid ◽  
Gene Delivery ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Viral Gene ◽  
Mechanism Study ◽  
High Affinity ◽  
Gene Vectors

The development of cationic non-viral gene vectors that may overcome the obstacles in gene delivery is of great significance to gene therapy. Metallic complexes with high affinity to nucleic acid...

Synthesis and gene transfection activity of cyclen-based cationic lipids with asymmetric acyl-cholesteryl hydrophobic tails

2014 ◽  
Vol 12 (21) ◽  
pp. 3484-3492 ◽  
Author(s):  
Bao-Quan Liu ◽  
Wen-Jing Yi ◽  
Ji Zhang ◽  
Qiang Liu ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Cationic Lipids ◽  
Viral Gene ◽  
Gene Vectors

Novel cyclen-based cationic lipids with asymmetric acyl-cholesteryl hydrophobic tails were synthesized and applied as non-viral gene vectors.

Flexible cyclic siloxane core enhances the transfection efficiency of polyethylenimine-based non-viral gene vectors

2015 ◽  
Vol 3 (42) ◽  
pp. 8250-8267 ◽  
Author(s):  
Cristina M. Uritu ◽  
Manuela Calin ◽  
Stelian S. Maier ◽  
Corneliu Cojocaru ◽  
Alina Nicolescu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Viral Gene ◽  
Cyclic Siloxane ◽  
Gene Vectors

cD4H–AGE–PEI conjugates, with a favorable balance between hydrophilic and hydrophobic moieties, are promising carriers for gene delivery.

Self-assembled CaP-based hybrid nanoparticles to enhance gene transfection efficiency in vitro and in vivo: beneficial utilization of PEGylated bisphosphate and nucleus locating signal

2018 ◽  
Vol 6 (21) ◽  
pp. 3466-3474 ◽  
Author(s):  
Wenpan Li ◽  
Dan Liu ◽  
Qiqi Wang ◽  
Haiyang Hu ◽  
Dawei Chen
Keyword(s):  
Calcium Phosphate ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Hybrid Nanoparticles ◽  
Viral Gene ◽  
Delivery Vehicle ◽  
Gene Delivery Vehicle ◽  
Viral Gene Delivery

Calcium phosphate (CaP) nanoparticles have been considered as a non-viral gene delivery vehicle, but the weakness of inconsistent and low transfection efficiencies is limited to its progress.

Disulfide modified self-assembly of lipopeptides with arginine-rich periphery achieve excellent gene transfection efficiency at relatively low nitrogen to phosphorus ratios

2017 ◽  
Vol 5 (7) ◽  
pp. 1482-1497 ◽  
Author(s):  
Xiaobing Chen ◽  
Jun Yang ◽  
Hong Liang ◽  
Qian Jiang ◽  
Bowen Ke ◽  
...  
Keyword(s):  
Self Assembly ◽  
Disulfide Bonds ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Viral Envelope ◽  
Self Assembled ◽  
Low Nitrogen

Self-assembled lipopeptides, with viral envelope, capsid-inspired arginine-rich periphery and disulfide bonds, achieve excellent transfectionin vitroandin vivo.

Glutathione modified low molecular weight PEI for highly improved gene transfection ability and biocompatibility

2019 ◽  
Vol 43 (30) ◽  
pp. 12109-12117
Author(s):  
Yu-Rong Zhan ◽  
Qing-Ying Yu ◽  
Ji Zhang ◽  
Yan-Hong Liu ◽  
Ya-Ping Xiao ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Low Molecular Weight ◽  
Gene Vectors

A versatile oligopeptide, glutathione, was introduced to construct novel cationic gene vectors with further excellent transfection efficiency and serum tolerance.

Synthesis of bifunctional molecules containing [12]aneN3 and coumarin moieties as effective DNA condensation agents and new non-viral gene vectors

2015 ◽  
Vol 13 (15) ◽  
pp. 4494-4505 ◽  
Author(s):  
Pan Yue ◽  
Ying Zhang ◽  
Zhi-Fo Guo ◽  
Ao-Cheng Cao ◽  
Zhong-Lin Lu ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Dna Condensation ◽  
Viral Gene ◽  
Bifunctional Molecules ◽  
Gene Vectors

Bifunctional molecules with different combinations of [12]aneN3 and coumarin moieties were successfully applied in DNA condensation and gene transfection.

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