Three-dimensional Growth of Renal Epithelial Cells in Vitro: A Tool in Toxicity Testing
1993 ◽
Vol 21
(2)
◽
pp. 191-195
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Keyword(s):
The tissue culture of multicellular spheroids from the renal epithelial cell line LLC-PK1 (proximal tubule) is described. This represents a biological system of intermediate complexity between renal tissue in vivo and simple monolayer cultures. The multicellular structures, which show many similarities to kidney tubules in vivo, including a vectorial water transport, should prove useful for studying the potential nephrotoxicity of drugs and chemicals in vitro. In addition, the propagation of renal epithelial cells as multicellular spheroids in serum-free culture may provide information on the release of specific biological parameters, which may be suppressed or masked in serum-supplemented media.
2006 ◽
Vol 290
(2)
◽
pp. C492-C498
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2012 ◽
Vol 303
(8)
◽
pp. C862-C871
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Keyword(s):
Keyword(s):
1991 ◽
Vol 260
(6)
◽
pp. F906-F912
◽
Keyword(s):
1982 ◽
Vol 95
(1)
◽
pp. 333-339
◽
Keyword(s):
Keyword(s):