Simultaneous detection of parathion and imidacloprid using broad-specificity polyclonal antibody in enzyme-linked immunosorbent assay

2015 ◽  
Vol 7 (1) ◽  
pp. 205-210 ◽  
Author(s):  
Lening Zhang ◽  
Zhanfeng Wang ◽  
Yubin Wen ◽  
Jingwei Shi ◽  
Jincheng Wang
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Simultaneous Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Broad Specificity

A heterologous indirect competitive enzyme-linked immunosorbent assay for the gross determination of parathion and imidacloprid residues was developed.

Highly selective and sensitive determination of deoxymiroestrol using a polyclonal antibody-based enzyme-linked immunosorbent assay

Talanta ◽  
2013 ◽  
Vol 114 ◽  
pp. 73-78 ◽  
Author(s):  
Gorawit Yusakul ◽  
Orapin Udomsin ◽  
Thaweesak Juengwatanatrakul ◽  
Hiroyuki Tanaka ◽  
Chaiyo Chaichantipyuth ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sensitive Determination

scholarly journals Enzyme-linked immunosorbent assay for the determination of isoflavones in alimentary important plants

2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S199-S202 ◽  
Author(s):  
M. Vítková ◽  
Z. Macková ◽  
R. Koblovská ◽  
O. Lapčík
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Medicago Sativa ◽  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Bovine Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Acceptable Range ◽  
Carboxy Methyl

The development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin and their homologues, is presented in this work. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4’ via a carboxy methyl spacer. The developed ELISAs are highly specific, I<sub>50</sub> values of the standard curves range between 0.3–1.2 ng/ml. The cross reactivities to other isoflavones are in acceptable range and the interference of non-isoflavonoid molecules is negligible. The immunoassays have been used for monitoring of changes in isoflavone levels in alimentary important plants, such as Medicago sativa, during germination; and during different vegetation stages of the Rutaceae family plants.

scholarly journals Simultaneous Determination of Florfenicol and Its Metabolite Florfenicol Amine in Swine Muscle Tissue by a Heterologous Enzyme-Linked Immunosorbent Assay

2009 ◽  
Vol 92 (3) ◽  
pp. 981-988 ◽  
Author(s):  
Pengjie Luo ◽  
Haiyang Jiang ◽  
Zhanhui Wang ◽  
Caimao Feng ◽  
Fangyang He ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Muscle Tissue ◽  
Immunosorbent Assay ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A sensitive and heterologous enzyme-linked immunosorbent assay (ELISA) for the simultaneous detection of florfenicol (FF) and its metabolite florfenicol amine (FFA) in swine muscle tissue was developed. FFA was conjugated to bovine serum albumin by a formaldehyde coupling method as an immunogen to immunize rabbits. FFA, thiamphenicol glycinate, and modified FF were conjugated to ovalbumin as coating antigens. The effect of different types of hapten heterology on the sensitivity and specificity of the ELISA was evaluated. Using FF glutaric anhydride ester as a coating hapten and antibody raised against modified FFA, an ELISA was developed that showed an IC50 value of 0.48 ng/mL. The antibody showed a cross-reactivity of 100 with FFA, 97 with FF, 6 with thiamphenicol, and a negligible value with chloramphenicol. From fortified swine muscle samples at levels of 4320 ng/g, the average recoveries of FF and FFA ranged from 58.2 to 96.8, with coefficients of variation less than 14. Analysis of incurred samples by the ELISA gave similar results to those by a previously developed liquid chromatographic method. The ELISA could be used as a rapid method for the simultaneous determination of FF and FFA in swine muscle tissue.

A new residue method for the determination of flonicamid in agricultural and environmental samples using enzyme immunoassay systems

RSC Advances ◽  
2016 ◽  
Vol 6 (42) ◽  
pp. 35842-35846 ◽  
Author(s):  
Zhenjiang Liu ◽  
Kewei Ren ◽  
Ming Li ◽  
Jiagao Wang ◽  
Jianfan Sun ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Environmental Samples ◽  
Enzyme Linked Immunosorbent Assay

An indirect competitive enzyme-linked immunosorbent assay (ELISA) for flonicamid was developed based on a polyclonal antibody.

A sensitive heterogeneous biotin–streptavidin enzyme-linked immunosorbent assay for the determination of di-(2-ethylhexyl)phthalate (DEHP) in beverages using a specific polyclonal antibody

2014 ◽  
Vol 6 (24) ◽  
pp. 9807-9815 ◽  
Author(s):  
Ruiyan Sun ◽  
Huisheng Zhuang
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Phthalic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ethylhexyl Phthalate ◽  
Specific Polyclonal Antibody ◽  
Long Chain

Di-(2-ethylhexyl)phthalate (DEHP) is one of the long-chain or high-molecular-weight compounds of the phthalic acid diester (PAEs) family, which is the most commonly used plasticizer and additive.

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