A protocol for rapid, label-free histochemical imaging of fibrotic liver

The Analyst ◽  
2017 ◽  
Vol 142 (8) ◽  
pp. 1179-1184 ◽  
Author(s):  
B. Bird ◽  
J. Rowlette
Keyword(s):  
High Throughput ◽  
Imaging Technique ◽  
Spectroscopic Imaging ◽  
Label Free ◽  
Biochemical Screening ◽  
Infrared Microscopy ◽  
Fibrotic Liver ◽  
Mid Infrared ◽  
Non Destructive

Mid-infrared microscopy is a non-destructive, quantitative and label-free spectroscopic imaging technique that, as a result of recent instrument advancements, is now at the point of enabling high-throughput automated biochemical screening of whole histology samples.

scholarly journals Spectroscopic Imaging with an Ultra-Broadband (1–4 THz) Compact Terahertz Difference-Frequency Generation Source

Electronics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 336
Author(s):  
Atsushi Nakanishi ◽  
Shohei Hayashi ◽  
Hiroshi Satozono ◽  
Kazuue Fujita
Keyword(s):  
Imaging Technique ◽  
Spectroscopic Imaging ◽  
Difference Frequency Generation ◽  
Difference Frequency ◽  
Quality Monitoring ◽  
Terahertz Emission ◽  
Mid Infrared ◽  
Quantum Cascade ◽  
Frequency Generation ◽  
Multi Mode

We demonstrate spectroscopic imaging using a compact ultra-broadband terahertz semiconductor source with a high-power, mid-infrared quantum cascade laser. The electrically pumped monolithic source is based on intra-cavity difference-frequency generation and can be designed to achieve an ultra-broadband multi-mode terahertz emission spectrum extending from 1–4 THz without any external optical setup. Spectroscopic imaging was performed with three frequency bands, 2.0 THz, 2.5 THz and 3.0 THz, and as a result, this imaging technique clearly identified three different tablet components (polyethylene, D-histidine and DL-histidine). This method may be highly suitable for quality monitoring of pharmaceutical materials.

scholarly journals Mid-infrared optoacoustic microscopy with label-free chemical contrast in living cells and tissues

2018 ◽  
Author(s):  
Miguel A. Pleitez ◽  
Asrar Ali Khan ◽  
Josefine Reber ◽  
Andriy Chmyrov ◽  
Markus R. Seeger ◽  
...  
Keyword(s):  
Imaging Modality ◽  
Living Cells ◽  
Spatiotemporal Dynamics ◽  
Live Cell ◽  
Imaging Modalities ◽  
Label Free ◽  
Infrared Microscopy ◽  
Mid Infrared ◽  
Infrared Sensing ◽  
Biomolecular Imaging

We developed mid-infrared optoacoustic microscopy (MiROM), a bond-selective imaging modality that overcomes water/tissue opacity and depth limitations of mid-infrared sensing allowing uncompromised live-cell/thick-tissue mid-infrared microscopy with up to three orders of magnitudehigher sensitivity than other vibrational imaging modalities; such as Raman. We showcase the functional label-free biomolecular imaging capabilities of MiROM by monitoring the spatiotemporal dynamics of lipids and proteins during lipolysis in living adipocytes. Since MiROM, contrary to Ramanmodalities, is not only able to detect lipids and proteins, but also important metabolites such as glucose without the need of labels, here we discuss how MiROM yields novel functional label-free abilities for a broader range of analytical studies in living cells and tissues.

Advancements in quantum cascade laser-based infrared microscopy of aqueous media

2016 ◽  
Vol 187 ◽  
pp. 119-134 ◽  
Author(s):  
K. Haase ◽  
N. Kröger-Lui ◽  
A. Pucci ◽  
A. Schönhals ◽  
W. Petrich
Keyword(s):  
Quantum Cascade Laser ◽  
Quantum Cascade Lasers ◽  
Spectral Power ◽  
Aqueous Media ◽  
Aqueous Environment ◽  
Label Free ◽  
Infrared Microscopy ◽  
Signal To Noise ◽  
Mid Infrared ◽  
Quantum Cascade

The large mid-infrared absorption coefficient of water frequently hampers the rapid, label-free infrared microscopy of biological objects in their natural aqueous environment. However, the high spectral power density of quantum cascade lasers is shifting this limitation such that mid-infrared absorbance images can be acquired in situ within signal-to-noise ratios of up to 100. Even at sample thicknesses well above 50 μm, signal-to-noise ratios above 10 are readily achieved. The quantum cascade laser-based microspectroscopy of aqueous media is exemplified by imaging an aqueous yeast solution and quantifying glucose consumption, ethanol generation as well as the production of carbon dioxide gas during fermentation.

Spectroscopic Imaging in the Mid-Infrared Applied to High-Throughput Studies of Supported Catalyst Libraries

2003 ◽  
pp. 77-91 ◽  
Author(s):  
Steven S. Lasko ◽  
Reed J. Hendershot ◽  
Yu Fu ◽  
Mark-Florian Fellmann ◽  
Gudbjorg Oskarsdottir ◽  
...  
Keyword(s):  
High Throughput ◽  
Supported Catalyst ◽  
Spectroscopic Imaging ◽  
Mid Infrared

High definition infrared chemical imaging of colorectal tissue using a Spero QCL microscope

The Analyst ◽  
2017 ◽  
Vol 142 (8) ◽  
pp. 1381-1386 ◽  
Author(s):  
B. Bird ◽  
J. Rowlette
Keyword(s):  
High Throughput ◽  
Chemical Imaging ◽  
Biomedical Science ◽  
Infrared Microscopy ◽  
High Definition ◽  
Mid Infrared ◽  
Colorectal Tissue ◽  
Infrared Microscope

Mid-infrared microscopy has become a key technique in the field of biomedical science and spectroscopy. In this current study, we explore the use of a QCL infrared microscope to produce high definition, high throughput chemical images useful for the screening of biopsied colorectal tissue.

scholarly journals Ultra-wideband enhancement on mid-infrared fingerprint sensing for 2D materials and analytes of monolayers by a metagrating

Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2927-2935
Author(s):  
Yinong Xie ◽  
Xueying Liu ◽  
Fajun Li ◽  
Jinfeng Zhu ◽  
Naixing Feng
Keyword(s):  
Trace Amount ◽  
2D Materials ◽  
Polarized Light ◽  
Ultra Wideband ◽  
Label Free ◽  
Infrared Band ◽  
Mid Infrared ◽  
Infrared Sensing ◽  
New Applications ◽  
Non Destructive

AbstractMid-infrared absorption spectroscopy is a powerful tool to identify analytes by detecting their material fingerprint in a label-free way, but it faces barriers on trace-amount analysis due to the difficulties in enhancing the broadband spectral signals. Here, we propose a sensing scheme based on the angular scanning of polarized light on a dielectric metagrating, and demonstrate it by numerical simulation. This approach not only indicates a series of significant signal enhancement factors over 30 times in an ultra-wide mid-infrared band, but also enables the explicit identification for various analytes, including 2D materials and trace-amount thin film samples. Our method would facilitate mid-infrared sensing for 2D materials and trace-amount analysis, and enable many new applications on non-destructive molecular identification.

scholarly journals Depth-resolved mid-infrared photothermal imaging of living cells and organisms with submicrometer spatial resolution

2016 ◽  
Vol 2 (9) ◽  
pp. e1600521 ◽  
Author(s):  
Delong Zhang ◽  
Chen Li ◽  
Chi Zhang ◽  
Mikhail N. Slipchenko ◽  
Gregory Eakins ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Single Cells ◽  
Chemical Imaging ◽  
Detection Sensitivity ◽  
Living Systems ◽  
Live Cells ◽  
Photothermal Effect ◽  
Label Free ◽  
Infrared Microscopy ◽  
Mid Infrared

Chemical contrast has long been sought for label-free visualization of biomolecules and materials in complex living systems. Although infrared spectroscopic imaging has come a long way in this direction, it is thus far only applicable to dried tissues because of the strong infrared absorption by water. It also suffers from low spatial resolution due to long wavelengths and lacks optical sectioning capabilities. We overcome these limitations through sensing vibrational absorption–induced photothermal effect by a visible laser beam. Our mid-infrared photothermal (MIP) approach reached 10 μM detection sensitivity and submicrometer lateral spatial resolution. This performance has exceeded the diffraction limit of infrared microscopy and allowed label-free three-dimensional chemical imaging of live cells and organisms. Distributions of endogenous lipid and exogenous drug inside single cells were visualized. We further demonstrated in vivo MIP imaging of lipids and proteins inCaenorhabditis elegans. The reported MIP imaging technology promises broad applications from monitoring metabolic activities to high-resolution mapping of drug molecules in living systems, which are beyond the reach of current infrared microscopy.

Spectroscopic Imaging in the Mid-Infrared Applied to High-Throughput Studies of Supported Catalyst Libraries

ChemInform ◽  
2004 ◽  
Vol 35 (36) ◽  
Author(s):  
Steven S. Lasko ◽  
Reed J. Hendershot ◽  
Yu Fu ◽  
Mark-Florian Fellmann ◽  
Gudbjorg Oskarsdottir ◽  
...  
Keyword(s):  
High Throughput ◽  
Supported Catalyst ◽  
Spectroscopic Imaging ◽  
Mid Infrared
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